Experiment: spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0462 and Dsui_0463 are separated by 47 nucleotides Dsui_0463 and Dsui_0464 are separated by 6 nucleotides Dsui_0464 and Dsui_0465 overlap by 14 nucleotides
Dsui_0462: Dsui_0462 - ADP-heptose:LPS heptosyltransferase, at 467,916 to 468,938
_0462
Dsui_0463: Dsui_0463 - hypothetical protein, at 468,986 to 470,323
_0463
Dsui_0464: Dsui_0464 - hypothetical protein, at 470,330 to 471,286
_0464
Dsui_0465: Dsui_0465 - ADP-heptose:LPS heptosyltransferase, at 471,273 to 472,451
_0465
Position (kb) 468
469
470
471 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 468.018 kb on - strand at 468.026 kb on - strand, within Dsui_0462 at 468.134 kb on - strand, within Dsui_0462 at 468.214 kb on - strand, within Dsui_0462 at 468.257 kb on - strand, within Dsui_0462 at 468.347 kb on - strand, within Dsui_0462 at 468.362 kb on - strand, within Dsui_0462 at 468.363 kb on - strand, within Dsui_0462 at 468.445 kb on + strand, within Dsui_0462 at 468.457 kb on - strand, within Dsui_0462 at 468.527 kb on + strand, within Dsui_0462 at 468.576 kb on + strand, within Dsui_0462 at 468.665 kb on - strand, within Dsui_0462 at 468.709 kb on - strand, within Dsui_0462 at 468.719 kb on + strand, within Dsui_0462 at 468.732 kb on + strand, within Dsui_0462 at 468.949 kb on + strand at 469.009 kb on - strand at 469.071 kb on - strand at 469.080 kb on + strand at 469.088 kb on - strand at 469.088 kb on - strand at 469.207 kb on + strand, within Dsui_0463 at 469.312 kb on - strand, within Dsui_0463 at 469.323 kb on - strand, within Dsui_0463 at 469.333 kb on - strand, within Dsui_0463 at 469.386 kb on - strand at 469.501 kb on - strand, within Dsui_0463 at 469.513 kb on + strand, within Dsui_0463 at 469.555 kb on + strand, within Dsui_0463 at 469.579 kb on - strand, within Dsui_0463 at 469.588 kb on + strand, within Dsui_0463 at 469.722 kb on - strand, within Dsui_0463 at 469.761 kb on + strand, within Dsui_0463 at 469.785 kb on - strand at 469.818 kb on + strand, within Dsui_0463 at 469.858 kb on + strand, within Dsui_0463 at 469.910 kb on - strand, within Dsui_0463 at 469.920 kb on - strand, within Dsui_0463 at 469.940 kb on - strand, within Dsui_0463 at 470.006 kb on - strand, within Dsui_0463 at 470.043 kb on - strand at 470.086 kb on - strand, within Dsui_0463 at 470.097 kb on + strand, within Dsui_0463 at 470.216 kb on + strand at 470.275 kb on - strand at 470.366 kb on + strand at 470.446 kb on - strand, within Dsui_0464 at 470.679 kb on + strand, within Dsui_0464 at 470.707 kb on - strand, within Dsui_0464 at 470.757 kb on + strand at 470.758 kb on - strand, within Dsui_0464 at 470.818 kb on - strand, within Dsui_0464 at 470.893 kb on + strand, within Dsui_0464 at 470.918 kb on + strand, within Dsui_0464 at 470.973 kb on - strand, within Dsui_0464 at 471.108 kb on - strand, within Dsui_0464 at 471.157 kb on + strand at 471.168 kb on - strand, within Dsui_0464 at 471.236 kb on - strand at 471.271 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 468,018 - -3.5 468,026 - Dsui_0462 0.11 -3.3 468,134 - Dsui_0462 0.21 -2.8 468,214 - Dsui_0462 0.29 -1.6 468,257 - Dsui_0462 0.33 -2.1 468,347 - Dsui_0462 0.42 -4.1 468,362 - Dsui_0462 0.44 -2.7 468,363 - Dsui_0462 0.44 -2.6 468,445 + Dsui_0462 0.52 -1.9 468,457 - Dsui_0462 0.53 -2.2 468,527 + Dsui_0462 0.60 -1.6 468,576 + Dsui_0462 0.65 -2.2 468,665 - Dsui_0462 0.73 -2.6 468,709 - Dsui_0462 0.78 -1.7 468,719 + Dsui_0462 0.78 -2.6 468,732 + Dsui_0462 0.80 -1.4 468,949 + +2.1 469,009 - -3.0 469,071 - -1.3 469,080 + -1.7 469,088 - -2.9 469,088 - -3.9 469,207 + Dsui_0463 0.17 -4.3 469,312 - Dsui_0463 0.24 -3.6 469,323 - Dsui_0463 0.25 -2.2 469,333 - Dsui_0463 0.26 -2.9 469,386 - -1.7 469,501 - Dsui_0463 0.38 -2.9 469,513 + Dsui_0463 0.39 -3.3 469,555 + Dsui_0463 0.43 -1.6 469,579 - Dsui_0463 0.44 -0.7 469,588 + Dsui_0463 0.45 -1.6 469,722 - Dsui_0463 0.55 -3.3 469,761 + Dsui_0463 0.58 -2.2 469,785 - -3.7 469,818 + Dsui_0463 0.62 -1.5 469,858 + Dsui_0463 0.65 -3.2 469,910 - Dsui_0463 0.69 -2.2 469,920 - Dsui_0463 0.70 -2.2 469,940 - Dsui_0463 0.71 -5.0 470,006 - Dsui_0463 0.76 -3.5 470,043 - -0.8 470,086 - Dsui_0463 0.82 +0.2 470,097 + Dsui_0463 0.83 -1.9 470,216 + -3.0 470,275 - +3.2 470,366 + -0.7 470,446 - Dsui_0464 0.12 -1.3 470,679 + Dsui_0464 0.36 -2.6 470,707 - Dsui_0464 0.39 -1.2 470,757 + -2.6 470,758 - Dsui_0464 0.45 -1.8 470,818 - Dsui_0464 0.51 -1.7 470,893 + Dsui_0464 0.59 -0.3 470,918 + Dsui_0464 0.61 -4.9 470,973 - Dsui_0464 0.67 -3.2 471,108 - Dsui_0464 0.81 -0.5 471,157 + -2.9 471,168 - Dsui_0464 0.88 -3.5 471,236 - -1.0 471,271 + -2.4
Or see this region's nucleotide sequence