Experiment: spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0457 and Dsui_0458 are separated by 44 nucleotides Dsui_0458 and Dsui_0459 are separated by 26 nucleotides Dsui_0459 and Dsui_0460 are separated by 25 nucleotides Dsui_0460 and Dsui_0461 overlap by 4 nucleotides
Dsui_0457: Dsui_0457 - Protein of unknown function (DUF2782), at 461,826 to 462,176
_0457
Dsui_0458: Dsui_0458 - TIGR00730 family protein, at 462,221 to 462,949
_0458
Dsui_0459: Dsui_0459 - DNA polymerase I, at 462,976 to 465,708
_0459
Dsui_0460: Dsui_0460 - ADP-heptose:LPS heptosyltransferase, at 465,734 to 466,699
_0460
Dsui_0461: Dsui_0461 - glycosyltransferase, at 466,696 to 467,832
_0461
Position (kb) 462
463
464
465
466 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 462.132 kb on + strand, within Dsui_0457 at 462.243 kb on - strand at 462.254 kb on - strand at 462.259 kb on + strand at 462.267 kb on - strand at 462.273 kb on + strand at 462.329 kb on + strand, within Dsui_0458 at 462.334 kb on + strand, within Dsui_0458 at 462.334 kb on + strand at 462.342 kb on - strand, within Dsui_0458 at 462.342 kb on - strand, within Dsui_0458 at 462.492 kb on - strand, within Dsui_0458 at 462.517 kb on + strand at 462.525 kb on - strand, within Dsui_0458 at 462.601 kb on - strand, within Dsui_0458 at 462.728 kb on - strand, within Dsui_0458 at 462.754 kb on + strand, within Dsui_0458 at 462.762 kb on - strand, within Dsui_0458 at 462.774 kb on + strand at 462.791 kb on + strand, within Dsui_0458 at 462.819 kb on + strand at 462.827 kb on - strand, within Dsui_0458 at 462.887 kb on - strand at 462.914 kb on - strand at 462.917 kb on + strand at 463.824 kb on + strand, within Dsui_0459 at 464.259 kb on + strand, within Dsui_0459 at 465.115 kb on + strand, within Dsui_0459 at 465.178 kb on - strand at 465.213 kb on + strand, within Dsui_0459 at 465.545 kb on + strand at 465.812 kb on + strand at 465.820 kb on - strand at 465.888 kb on - strand, within Dsui_0460 at 465.940 kb on - strand, within Dsui_0460 at 466.153 kb on + strand at 466.448 kb on + strand, within Dsui_0460 at 466.642 kb on + strand at 466.650 kb on + strand at 466.650 kb on + strand at 466.650 kb on + strand at 466.650 kb on + strand at 466.652 kb on + strand at 466.658 kb on - strand at 466.658 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 462,132 + Dsui_0457 0.87 -2.1 462,243 - +0.0 462,254 - +0.7 462,259 + -2.7 462,267 - +0.4 462,273 + -3.9 462,329 + Dsui_0458 0.15 -2.5 462,334 + Dsui_0458 0.16 -1.7 462,334 + -1.7 462,342 - Dsui_0458 0.17 -2.1 462,342 - Dsui_0458 0.17 -0.2 462,492 - Dsui_0458 0.37 -1.0 462,517 + -2.5 462,525 - Dsui_0458 0.42 -0.1 462,601 - Dsui_0458 0.52 -0.7 462,728 - Dsui_0458 0.70 -1.2 462,754 + Dsui_0458 0.73 -0.8 462,762 - Dsui_0458 0.74 -1.0 462,774 + -2.4 462,791 + Dsui_0458 0.78 -2.9 462,819 + -2.1 462,827 - Dsui_0458 0.83 -2.1 462,887 - -0.8 462,914 - -0.7 462,917 + -1.9 463,824 + Dsui_0459 0.31 +0.3 464,259 + Dsui_0459 0.47 -3.1 465,115 + Dsui_0459 0.78 +0.3 465,178 - -0.2 465,213 + Dsui_0459 0.82 -1.7 465,545 + +1.1 465,812 + -3.4 465,820 - -1.5 465,888 - Dsui_0460 0.16 -0.7 465,940 - Dsui_0460 0.21 -0.9 466,153 + -2.0 466,448 + Dsui_0460 0.74 -3.0 466,642 + -2.9 466,650 + -2.7 466,650 + -3.9 466,650 + -4.2 466,650 + -3.4 466,652 + -3.1 466,658 - -1.9 466,658 - -3.5
Or see this region's nucleotide sequence