Experiment: spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0258 and Dsui_0259 are separated by 10 nucleotides Dsui_0259 and Dsui_0260 overlap by 4 nucleotides Dsui_0260 and Dsui_0261 are separated by 74 nucleotides
Dsui_0258: Dsui_0258 - sulfate ABC transporter, ATP-binding protein, at 255,855 to 256,937
_0258
Dsui_0259: Dsui_0259 - sulfate ABC transporter, permease protein CysW, at 256,948 to 257,814
_0259
Dsui_0260: Dsui_0260 - sulfate ABC transporter, permease protein CysT, at 257,811 to 258,659
_0260
Dsui_0261: Dsui_0261 - sulfate/thiosulfate-binding protein, at 258,734 to 259,747
_0261
Position (kb) 256
257
258 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 256.030 kb on + strand, within Dsui_0258 at 256.093 kb on + strand, within Dsui_0258 at 256.131 kb on + strand, within Dsui_0258 at 256.178 kb on + strand, within Dsui_0258 at 256.178 kb on + strand, within Dsui_0258 at 256.435 kb on - strand, within Dsui_0258 at 256.494 kb on + strand at 256.565 kb on + strand, within Dsui_0258 at 256.570 kb on + strand, within Dsui_0258 at 256.588 kb on - strand, within Dsui_0258 at 256.667 kb on + strand, within Dsui_0258 at 256.726 kb on + strand, within Dsui_0258 at 256.844 kb on - strand at 256.971 kb on + strand at 256.991 kb on + strand at 257.125 kb on - strand, within Dsui_0259 at 257.129 kb on + strand, within Dsui_0259 at 257.137 kb on - strand, within Dsui_0259 at 257.141 kb on + strand, within Dsui_0259 at 257.301 kb on + strand, within Dsui_0259 at 257.306 kb on + strand, within Dsui_0259 at 257.362 kb on - strand, within Dsui_0259 at 257.443 kb on + strand, within Dsui_0259 at 257.478 kb on - strand, within Dsui_0259 at 257.768 kb on + strand at 257.774 kb on - strand at 257.835 kb on - strand at 257.911 kb on + strand, within Dsui_0260 at 257.946 kb on - strand, within Dsui_0260 at 257.946 kb on - strand, within Dsui_0260 at 258.056 kb on + strand, within Dsui_0260 at 258.069 kb on + strand, within Dsui_0260 at 258.190 kb on - strand, within Dsui_0260 at 258.190 kb on - strand, within Dsui_0260 at 258.245 kb on - strand, within Dsui_0260 at 258.245 kb on - strand, within Dsui_0260 at 258.257 kb on + strand, within Dsui_0260 at 258.263 kb on + strand, within Dsui_0260 at 258.328 kb on - strand, within Dsui_0260 at 258.357 kb on - strand, within Dsui_0260 at 258.604 kb on + strand at 258.685 kb on - strand at 258.744 kb on + strand at 258.757 kb on - strand at 258.786 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 256,030 + Dsui_0258 0.16 -0.8 256,093 + Dsui_0258 0.22 -3.9 256,131 + Dsui_0258 0.25 -1.3 256,178 + Dsui_0258 0.30 -2.3 256,178 + Dsui_0258 0.30 -4.3 256,435 - Dsui_0258 0.54 -3.2 256,494 + +0.5 256,565 + Dsui_0258 0.66 -2.3 256,570 + Dsui_0258 0.66 -1.9 256,588 - Dsui_0258 0.68 -1.8 256,667 + Dsui_0258 0.75 -3.0 256,726 + Dsui_0258 0.80 -3.3 256,844 - -2.6 256,971 + -0.5 256,991 + +0.6 257,125 - Dsui_0259 0.20 -2.8 257,129 + Dsui_0259 0.21 -2.7 257,137 - Dsui_0259 0.22 -3.4 257,141 + Dsui_0259 0.22 -3.4 257,301 + Dsui_0259 0.41 -3.5 257,306 + Dsui_0259 0.41 -3.7 257,362 - Dsui_0259 0.48 -2.6 257,443 + Dsui_0259 0.57 -2.1 257,478 - Dsui_0259 0.61 -2.8 257,768 + -3.8 257,774 - -3.4 257,835 - +1.3 257,911 + Dsui_0260 0.12 -0.8 257,946 - Dsui_0260 0.16 -2.8 257,946 - Dsui_0260 0.16 -5.0 258,056 + Dsui_0260 0.29 -2.9 258,069 + Dsui_0260 0.30 -3.1 258,190 - Dsui_0260 0.45 -3.1 258,190 - Dsui_0260 0.45 -3.0 258,245 - Dsui_0260 0.51 -1.8 258,245 - Dsui_0260 0.51 -4.3 258,257 + Dsui_0260 0.53 -1.3 258,263 + Dsui_0260 0.53 -2.2 258,328 - Dsui_0260 0.61 -4.3 258,357 - Dsui_0260 0.64 -4.3 258,604 + -2.8 258,685 - +2.0 258,744 + -0.0 258,757 - +1.2 258,786 - -0.3
Or see this region's nucleotide sequence