Experiment: spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0082 and Dsui_0083 are separated by 2 nucleotides Dsui_0083 and Dsui_0084 are separated by 10 nucleotides
Dsui_0082: Dsui_0082 - UDP-4-keto-6-deoxy-N-acetylglucosamine 4-aminotransferase, at 79,093 to 80,253
_0082
Dsui_0083: Dsui_0083 - UDP-N-acetylglucosamine 4,6-dehydratase, at 80,256 to 81,254
_0083
Dsui_0084: Dsui_0084 - ABC-type bacteriocin/lantibiotic exporter with N-terminal double-glycine peptidase domain, at 81,265 to 83,040
_0084
Position (kb)
80
81
82 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 80.295 kb on + strand at 80.298 kb on - strand at 80.344 kb on - strand at 80.346 kb on + strand at 80.346 kb on + strand at 80.353 kb on - strand at 80.354 kb on - strand at 80.354 kb on - strand at 80.413 kb on + strand, within Dsui_0083 at 80.422 kb on + strand, within Dsui_0083 at 80.459 kb on - strand, within Dsui_0083 at 80.470 kb on + strand, within Dsui_0083 at 80.472 kb on + strand, within Dsui_0083 at 80.472 kb on - strand, within Dsui_0083 at 80.534 kb on - strand, within Dsui_0083 at 80.550 kb on - strand, within Dsui_0083 at 80.561 kb on + strand, within Dsui_0083 at 80.615 kb on + strand, within Dsui_0083 at 80.628 kb on - strand, within Dsui_0083 at 80.628 kb on - strand, within Dsui_0083 at 80.630 kb on + strand, within Dsui_0083 at 80.630 kb on + strand, within Dsui_0083 at 80.642 kb on + strand, within Dsui_0083 at 80.642 kb on + strand, within Dsui_0083 at 80.664 kb on + strand, within Dsui_0083 at 80.690 kb on + strand, within Dsui_0083 at 80.708 kb on - strand, within Dsui_0083 at 80.730 kb on - strand, within Dsui_0083 at 80.845 kb on + strand, within Dsui_0083 at 80.860 kb on - strand, within Dsui_0083 at 80.863 kb on + strand, within Dsui_0083 at 80.863 kb on + strand, within Dsui_0083 at 80.871 kb on - strand, within Dsui_0083 at 80.887 kb on + strand, within Dsui_0083 at 80.888 kb on - strand, within Dsui_0083 at 80.931 kb on - strand, within Dsui_0083 at 80.976 kb on + strand, within Dsui_0083 at 80.976 kb on + strand, within Dsui_0083 at 80.979 kb on - strand, within Dsui_0083 at 80.984 kb on - strand, within Dsui_0083 at 80.984 kb on - strand, within Dsui_0083 at 80.984 kb on - strand, within Dsui_0083 at 80.984 kb on - strand, within Dsui_0083 at 80.984 kb on - strand, within Dsui_0083 at 80.986 kb on + strand, within Dsui_0083 at 81.010 kb on + strand, within Dsui_0083 at 81.010 kb on + strand, within Dsui_0083 at 81.033 kb on + strand, within Dsui_0083 at 81.166 kb on + strand at 81.172 kb on + strand at 81.174 kb on - strand at 81.174 kb on - strand at 81.251 kb on + strand at 81.290 kb on - strand at 81.322 kb on - strand at 81.374 kb on - strand at 81.392 kb on + strand at 81.395 kb on + strand at 81.403 kb on - strand at 81.490 kb on - strand, within Dsui_0084 at 81.490 kb on - strand, within Dsui_0084 at 81.492 kb on - strand, within Dsui_0084 at 81.492 kb on - strand, within Dsui_0084 at 81.583 kb on - strand, within Dsui_0084 at 81.712 kb on - strand, within Dsui_0084 at 81.765 kb on + strand, within Dsui_0084 at 81.799 kb on + strand, within Dsui_0084 at 81.817 kb on + strand, within Dsui_0084 at 81.825 kb on - strand, within Dsui_0084 at 81.859 kb on - strand, within Dsui_0084 at 81.893 kb on + strand, within Dsui_0084 at 81.976 kb on + strand, within Dsui_0084 at 81.998 kb on + strand, within Dsui_0084 at 82.012 kb on - strand, within Dsui_0084 at 82.115 kb on + strand, within Dsui_0084 at 82.178 kb on - strand, within Dsui_0084 at 82.183 kb on - strand, within Dsui_0084 at 82.188 kb on + strand, within Dsui_0084 at 82.207 kb on + strand, within Dsui_0084
Per-strain Table
Position Strand Gene LocusTag Fraction spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 80,295 + -3.0 80,298 - -1.0 80,344 - -2.9 80,346 + +0.2 80,346 + -1.5 80,353 - -4.0 80,354 - -1.5 80,354 - -2.8 80,413 + Dsui_0083 0.16 -1.6 80,422 + Dsui_0083 0.17 -3.9 80,459 - Dsui_0083 0.20 -2.7 80,470 + Dsui_0083 0.21 -3.6 80,472 + Dsui_0083 0.22 -3.4 80,472 - Dsui_0083 0.22 -1.8 80,534 - Dsui_0083 0.28 -2.3 80,550 - Dsui_0083 0.29 -3.2 80,561 + Dsui_0083 0.31 -3.8 80,615 + Dsui_0083 0.36 -2.2 80,628 - Dsui_0083 0.37 -2.9 80,628 - Dsui_0083 0.37 -2.0 80,630 + Dsui_0083 0.37 +0.3 80,630 + Dsui_0083 0.37 -3.8 80,642 + Dsui_0083 0.39 -2.4 80,642 + Dsui_0083 0.39 -3.4 80,664 + Dsui_0083 0.41 -1.5 80,690 + Dsui_0083 0.43 -3.4 80,708 - Dsui_0083 0.45 -2.4 80,730 - Dsui_0083 0.47 -4.4 80,845 + Dsui_0083 0.59 -3.0 80,860 - Dsui_0083 0.60 -1.4 80,863 + Dsui_0083 0.61 -2.0 80,863 + Dsui_0083 0.61 -2.8 80,871 - Dsui_0083 0.62 -3.9 80,887 + Dsui_0083 0.63 -2.6 80,888 - Dsui_0083 0.63 -1.9 80,931 - Dsui_0083 0.68 -0.3 80,976 + Dsui_0083 0.72 -2.0 80,976 + Dsui_0083 0.72 -3.8 80,979 - Dsui_0083 0.72 -2.8 80,984 - Dsui_0083 0.73 -2.0 80,984 - Dsui_0083 0.73 -3.2 80,984 - Dsui_0083 0.73 -2.2 80,984 - Dsui_0083 0.73 -2.6 80,984 - Dsui_0083 0.73 -2.1 80,986 + Dsui_0083 0.73 +0.2 81,010 + Dsui_0083 0.75 -0.9 81,010 + Dsui_0083 0.75 -1.9 81,033 + Dsui_0083 0.78 -1.7 81,166 + -4.7 81,172 + -3.2 81,174 - -1.7 81,174 - -2.6 81,251 + +0.3 81,290 - +1.2 81,322 - +0.9 81,374 - -2.6 81,392 + -2.5 81,395 + -3.2 81,403 - -1.3 81,490 - Dsui_0084 0.13 -2.2 81,490 - Dsui_0084 0.13 -1.3 81,492 - Dsui_0084 0.13 -3.8 81,492 - Dsui_0084 0.13 -2.5 81,583 - Dsui_0084 0.18 -2.8 81,712 - Dsui_0084 0.25 -1.9 81,765 + Dsui_0084 0.28 -5.0 81,799 + Dsui_0084 0.30 -3.2 81,817 + Dsui_0084 0.31 -3.5 81,825 - Dsui_0084 0.32 -4.8 81,859 - Dsui_0084 0.33 -3.0 81,893 + Dsui_0084 0.35 -2.9 81,976 + Dsui_0084 0.40 -4.6 81,998 + Dsui_0084 0.41 -2.4 82,012 - Dsui_0084 0.42 -2.0 82,115 + Dsui_0084 0.48 -1.3 82,178 - Dsui_0084 0.51 -4.1 82,183 - Dsui_0084 0.52 -3.9 82,188 + Dsui_0084 0.52 -3.9 82,207 + Dsui_0084 0.53 -0.0
Or see this region's nucleotide sequence