Experiment: spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0074 and Dsui_0075 are separated by 149 nucleotides Dsui_0075 and Dsui_0076 overlap by 1 nucleotides Dsui_0076 and Dsui_0077 overlap by 4 nucleotides
Dsui_0074: Dsui_0074 - hypothetical protein, at 70,276 to 71,760
_0074
Dsui_0075: Dsui_0075 - hypothetical protein, at 71,910 to 72,812
_0075
Dsui_0076: Dsui_0076 - acetyltransferase, ribosomal protein N-acetylase, at 72,812 to 73,354
_0076
Dsui_0077: Dsui_0077 - putative PLP-dependent enzyme possibly involved in cell wall biogenesis, at 73,351 to 74,643
_0077
Position (kb) 71
72
73 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 70.943 kb on - strand, within Dsui_0074 at 70.950 kb on - strand, within Dsui_0074 at 70.950 kb on - strand, within Dsui_0074 at 71.037 kb on - strand, within Dsui_0074 at 71.053 kb on + strand, within Dsui_0074 at 71.053 kb on + strand, within Dsui_0074 at 71.061 kb on - strand, within Dsui_0074 at 71.105 kb on + strand, within Dsui_0074 at 71.118 kb on - strand, within Dsui_0074 at 71.123 kb on + strand, within Dsui_0074 at 71.131 kb on - strand, within Dsui_0074 at 71.186 kb on + strand, within Dsui_0074 at 71.207 kb on + strand, within Dsui_0074 at 71.207 kb on + strand, within Dsui_0074 at 71.209 kb on + strand, within Dsui_0074 at 71.217 kb on - strand, within Dsui_0074 at 71.217 kb on - strand, within Dsui_0074 at 71.245 kb on - strand, within Dsui_0074 at 71.250 kb on + strand, within Dsui_0074 at 71.256 kb on - strand, within Dsui_0074 at 71.270 kb on + strand, within Dsui_0074 at 71.278 kb on - strand, within Dsui_0074 at 71.295 kb on + strand, within Dsui_0074 at 71.297 kb on - strand, within Dsui_0074 at 71.309 kb on + strand, within Dsui_0074 at 71.309 kb on + strand, within Dsui_0074 at 71.309 kb on + strand, within Dsui_0074 at 71.309 kb on - strand, within Dsui_0074 at 71.310 kb on + strand, within Dsui_0074 at 71.315 kb on + strand, within Dsui_0074 at 71.317 kb on - strand, within Dsui_0074 at 71.317 kb on - strand, within Dsui_0074 at 71.328 kb on - strand, within Dsui_0074 at 71.333 kb on - strand, within Dsui_0074 at 71.427 kb on + strand, within Dsui_0074 at 71.455 kb on + strand, within Dsui_0074 at 71.509 kb on + strand, within Dsui_0074 at 71.695 kb on - strand at 71.757 kb on - strand at 71.764 kb on - strand at 71.804 kb on + strand at 71.856 kb on + strand at 71.856 kb on + strand at 71.856 kb on + strand at 71.856 kb on + strand at 71.856 kb on + strand at 71.864 kb on - strand at 71.864 kb on - strand at 71.864 kb on - strand at 71.864 kb on - strand at 71.864 kb on - strand at 71.864 kb on - strand at 71.886 kb on + strand at 71.886 kb on + strand at 71.887 kb on + strand at 71.887 kb on + strand at 71.894 kb on - strand at 71.894 kb on - strand at 71.913 kb on + strand at 71.970 kb on - strand at 71.970 kb on - strand at 72.032 kb on - strand, within Dsui_0075 at 72.039 kb on - strand, within Dsui_0075 at 72.051 kb on + strand, within Dsui_0075 at 72.051 kb on + strand, within Dsui_0075 at 72.051 kb on + strand, within Dsui_0075 at 72.051 kb on + strand, within Dsui_0075 at 72.051 kb on + strand, within Dsui_0075 at 72.051 kb on + strand, within Dsui_0075 at 72.052 kb on - strand, within Dsui_0075 at 72.059 kb on - strand, within Dsui_0075 at 72.059 kb on - strand, within Dsui_0075 at 72.060 kb on - strand, within Dsui_0075 at 72.111 kb on + strand, within Dsui_0075 at 72.119 kb on - strand, within Dsui_0075 at 72.119 kb on - strand, within Dsui_0075 at 72.203 kb on - strand, within Dsui_0075 at 72.206 kb on + strand, within Dsui_0075 at 72.209 kb on - strand, within Dsui_0075 at 72.209 kb on - strand, within Dsui_0075 at 72.218 kb on - strand, within Dsui_0075 at 72.222 kb on - strand, within Dsui_0075 at 72.256 kb on + strand, within Dsui_0075 at 72.256 kb on + strand, within Dsui_0075 at 72.295 kb on + strand, within Dsui_0075 at 72.304 kb on + strand, within Dsui_0075 at 72.371 kb on - strand, within Dsui_0075 at 72.376 kb on + strand, within Dsui_0075 at 72.376 kb on + strand, within Dsui_0075 at 72.441 kb on + strand, within Dsui_0075 at 72.442 kb on + strand, within Dsui_0075 at 72.442 kb on + strand, within Dsui_0075 at 72.467 kb on - strand, within Dsui_0075 at 72.473 kb on - strand, within Dsui_0075 at 72.476 kb on + strand, within Dsui_0075 at 72.496 kb on + strand, within Dsui_0075 at 72.498 kb on + strand, within Dsui_0075 at 72.498 kb on + strand, within Dsui_0075 at 72.504 kb on - strand, within Dsui_0075 at 72.508 kb on - strand, within Dsui_0075 at 72.565 kb on + strand, within Dsui_0075 at 72.573 kb on - strand, within Dsui_0075 at 72.612 kb on + strand, within Dsui_0075 at 72.643 kb on + strand, within Dsui_0075 at 72.648 kb on + strand, within Dsui_0075 at 72.648 kb on + strand, within Dsui_0075 at 72.651 kb on - strand, within Dsui_0075 at 72.656 kb on - strand, within Dsui_0075 at 72.656 kb on - strand, within Dsui_0075 at 72.690 kb on - strand, within Dsui_0075 at 72.728 kb on - strand at 72.738 kb on - strand at 72.765 kb on + strand at 72.773 kb on + strand at 72.840 kb on + strand at 72.840 kb on + strand at 72.840 kb on + strand at 72.840 kb on + strand at 72.843 kb on - strand at 72.848 kb on - strand at 72.848 kb on - strand at 72.848 kb on - strand at 72.865 kb on + strand at 72.865 kb on + strand at 72.865 kb on + strand at 72.873 kb on - strand, within Dsui_0076 at 72.895 kb on - strand, within Dsui_0076 at 72.897 kb on + strand, within Dsui_0076 at 72.918 kb on - strand, within Dsui_0076 at 72.918 kb on - strand, within Dsui_0076 at 72.934 kb on + strand, within Dsui_0076 at 72.937 kb on - strand, within Dsui_0076 at 72.991 kb on + strand, within Dsui_0076 at 73.038 kb on - strand, within Dsui_0076 at 73.053 kb on - strand, within Dsui_0076 at 73.076 kb on - strand, within Dsui_0076 at 73.081 kb on - strand, within Dsui_0076 at 73.131 kb on - strand, within Dsui_0076 at 73.131 kb on - strand, within Dsui_0076 at 73.164 kb on + strand, within Dsui_0076 at 73.164 kb on + strand, within Dsui_0076 at 73.172 kb on - strand, within Dsui_0076 at 73.185 kb on - strand, within Dsui_0076 at 73.190 kb on - strand, within Dsui_0076 at 73.194 kb on - strand, within Dsui_0076 at 73.285 kb on + strand, within Dsui_0076 at 73.287 kb on - strand, within Dsui_0076 at 73.293 kb on - strand, within Dsui_0076 at 73.293 kb on - strand, within Dsui_0076 at 73.295 kb on + strand, within Dsui_0076 at 73.303 kb on - strand at 73.313 kb on - strand at 73.370 kb on - strand at 73.373 kb on + strand at 73.376 kb on - strand at 73.381 kb on - strand at 73.381 kb on - strand at 73.381 kb on - strand at 73.381 kb on - strand at 73.425 kb on + strand at 73.435 kb on + strand at 73.501 kb on - strand, within Dsui_0077 at 73.509 kb on + strand, within Dsui_0077 at 73.532 kb on + strand, within Dsui_0077 at 73.532 kb on + strand, within Dsui_0077 at 73.545 kb on - strand, within Dsui_0077 at 73.593 kb on - strand, within Dsui_0077 at 73.618 kb on + strand, within Dsui_0077 at 73.639 kb on + strand, within Dsui_0077 at 73.642 kb on - strand, within Dsui_0077 at 73.674 kb on + strand, within Dsui_0077 at 73.681 kb on + strand, within Dsui_0077 at 73.689 kb on - strand, within Dsui_0077 at 73.689 kb on - strand, within Dsui_0077 at 73.689 kb on - strand, within Dsui_0077 at 73.689 kb on - strand, within Dsui_0077 at 73.697 kb on - strand, within Dsui_0077 at 73.710 kb on + strand, within Dsui_0077 at 73.731 kb on + strand, within Dsui_0077 at 73.739 kb on - strand, within Dsui_0077 at 73.739 kb on - strand, within Dsui_0077 at 73.739 kb on - strand, within Dsui_0077 at 73.798 kb on + strand, within Dsui_0077 at 73.812 kb on + strand, within Dsui_0077 at 73.812 kb on + strand, within Dsui_0077
Per-strain Table
Position Strand Gene LocusTag Fraction spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 70,943 - Dsui_0074 0.45 -3.9 70,950 - Dsui_0074 0.45 -3.7 70,950 - Dsui_0074 0.45 -1.5 71,037 - Dsui_0074 0.51 -1.9 71,053 + Dsui_0074 0.52 -3.8 71,053 + Dsui_0074 0.52 -2.6 71,061 - Dsui_0074 0.53 -1.4 71,105 + Dsui_0074 0.56 -5.4 71,118 - Dsui_0074 0.57 -1.3 71,123 + Dsui_0074 0.57 -1.9 71,131 - Dsui_0074 0.58 -2.2 71,186 + Dsui_0074 0.61 -2.3 71,207 + Dsui_0074 0.63 -3.7 71,207 + Dsui_0074 0.63 -2.5 71,209 + Dsui_0074 0.63 -4.7 71,217 - Dsui_0074 0.63 -3.2 71,217 - Dsui_0074 0.63 -1.9 71,245 - Dsui_0074 0.65 -2.9 71,250 + Dsui_0074 0.66 -2.7 71,256 - Dsui_0074 0.66 -2.0 71,270 + Dsui_0074 0.67 -6.3 71,278 - Dsui_0074 0.67 -2.2 71,295 + Dsui_0074 0.69 -0.8 71,297 - Dsui_0074 0.69 -4.6 71,309 + Dsui_0074 0.70 -4.2 71,309 + Dsui_0074 0.70 -1.9 71,309 + Dsui_0074 0.70 -1.6 71,309 - Dsui_0074 0.70 -1.1 71,310 + Dsui_0074 0.70 -3.9 71,315 + Dsui_0074 0.70 -1.0 71,317 - Dsui_0074 0.70 -3.5 71,317 - Dsui_0074 0.70 -2.6 71,328 - Dsui_0074 0.71 -1.5 71,333 - Dsui_0074 0.71 -2.8 71,427 + Dsui_0074 0.78 -1.9 71,455 + Dsui_0074 0.79 -3.8 71,509 + Dsui_0074 0.83 -4.5 71,695 - -4.2 71,757 - -2.5 71,764 - -2.0 71,804 + -1.0 71,856 + -2.5 71,856 + -0.5 71,856 + -4.8 71,856 + -2.0 71,856 + -3.2 71,864 - -0.0 71,864 - -3.8 71,864 - -2.3 71,864 - +1.1 71,864 - -2.8 71,864 - -2.4 71,886 + -2.8 71,886 + -3.7 71,887 + -3.7 71,887 + -0.5 71,894 - -0.4 71,894 - -0.8 71,913 + -0.4 71,970 - -1.6 71,970 - -3.0 72,032 - Dsui_0075 0.14 -1.7 72,039 - Dsui_0075 0.14 -2.7 72,051 + Dsui_0075 0.16 -4.6 72,051 + Dsui_0075 0.16 -2.4 72,051 + Dsui_0075 0.16 -2.7 72,051 + Dsui_0075 0.16 -4.1 72,051 + Dsui_0075 0.16 -0.8 72,051 + Dsui_0075 0.16 -3.3 72,052 - Dsui_0075 0.16 -0.6 72,059 - Dsui_0075 0.17 -2.9 72,059 - Dsui_0075 0.17 -1.3 72,060 - Dsui_0075 0.17 -1.3 72,111 + Dsui_0075 0.22 -2.6 72,119 - Dsui_0075 0.23 -2.1 72,119 - Dsui_0075 0.23 -1.5 72,203 - Dsui_0075 0.32 -0.3 72,206 + Dsui_0075 0.33 -0.0 72,209 - Dsui_0075 0.33 -1.0 72,209 - Dsui_0075 0.33 -2.2 72,218 - Dsui_0075 0.34 -2.2 72,222 - Dsui_0075 0.35 -2.4 72,256 + Dsui_0075 0.38 -3.9 72,256 + Dsui_0075 0.38 -3.3 72,295 + Dsui_0075 0.43 -2.5 72,304 + Dsui_0075 0.44 -2.3 72,371 - Dsui_0075 0.51 -1.3 72,376 + Dsui_0075 0.52 -4.3 72,376 + Dsui_0075 0.52 -3.5 72,441 + Dsui_0075 0.59 +1.1 72,442 + Dsui_0075 0.59 -4.3 72,442 + Dsui_0075 0.59 -2.6 72,467 - Dsui_0075 0.62 -1.8 72,473 - Dsui_0075 0.62 -1.1 72,476 + Dsui_0075 0.63 -2.5 72,496 + Dsui_0075 0.65 -1.7 72,498 + Dsui_0075 0.65 -3.5 72,498 + Dsui_0075 0.65 -2.7 72,504 - Dsui_0075 0.66 -2.0 72,508 - Dsui_0075 0.66 -3.8 72,565 + Dsui_0075 0.73 -3.4 72,573 - Dsui_0075 0.73 -1.5 72,612 + Dsui_0075 0.78 -2.9 72,643 + Dsui_0075 0.81 +1.1 72,648 + Dsui_0075 0.82 -3.2 72,648 + Dsui_0075 0.82 -3.2 72,651 - Dsui_0075 0.82 -1.5 72,656 - Dsui_0075 0.83 -0.5 72,656 - Dsui_0075 0.83 -1.3 72,690 - Dsui_0075 0.86 -1.1 72,728 - -2.2 72,738 - -1.7 72,765 + -2.1 72,773 + -2.6 72,840 + -1.4 72,840 + -5.6 72,840 + -4.5 72,840 + -3.8 72,843 - +1.6 72,848 - +1.0 72,848 - +1.0 72,848 - +0.7 72,865 + -4.1 72,865 + -3.1 72,865 + -3.7 72,873 - Dsui_0076 0.11 -3.2 72,895 - Dsui_0076 0.15 -2.6 72,897 + Dsui_0076 0.16 -2.0 72,918 - Dsui_0076 0.20 -4.4 72,918 - Dsui_0076 0.20 -1.9 72,934 + Dsui_0076 0.22 -3.4 72,937 - Dsui_0076 0.23 -3.0 72,991 + Dsui_0076 0.33 -3.5 73,038 - Dsui_0076 0.42 -4.3 73,053 - Dsui_0076 0.44 -1.8 73,076 - Dsui_0076 0.49 -3.1 73,081 - Dsui_0076 0.50 -2.2 73,131 - Dsui_0076 0.59 -2.6 73,131 - Dsui_0076 0.59 -3.5 73,164 + Dsui_0076 0.65 -1.8 73,164 + Dsui_0076 0.65 -3.3 73,172 - Dsui_0076 0.66 -0.5 73,185 - Dsui_0076 0.69 -5.0 73,190 - Dsui_0076 0.70 -4.2 73,194 - Dsui_0076 0.70 -3.0 73,285 + Dsui_0076 0.87 -1.1 73,287 - Dsui_0076 0.87 -2.4 73,293 - Dsui_0076 0.89 -2.4 73,293 - Dsui_0076 0.89 -0.5 73,295 + Dsui_0076 0.89 -1.8 73,303 - -4.8 73,313 - -2.5 73,370 - +0.5 73,373 + -5.0 73,376 - -2.9 73,381 - -2.1 73,381 - -0.9 73,381 - -1.0 73,381 - -1.0 73,425 + -1.5 73,435 + -2.2 73,501 - Dsui_0077 0.12 -4.0 73,509 + Dsui_0077 0.12 -4.2 73,532 + Dsui_0077 0.14 -5.2 73,532 + Dsui_0077 0.14 -5.3 73,545 - Dsui_0077 0.15 -3.1 73,593 - Dsui_0077 0.19 -4.0 73,618 + Dsui_0077 0.21 -3.2 73,639 + Dsui_0077 0.22 -3.4 73,642 - Dsui_0077 0.23 -4.9 73,674 + Dsui_0077 0.25 -2.5 73,681 + Dsui_0077 0.26 -5.1 73,689 - Dsui_0077 0.26 +0.5 73,689 - Dsui_0077 0.26 -2.8 73,689 - Dsui_0077 0.26 -3.2 73,689 - Dsui_0077 0.26 -2.8 73,697 - Dsui_0077 0.27 -2.7 73,710 + Dsui_0077 0.28 -4.0 73,731 + Dsui_0077 0.29 -3.8 73,739 - Dsui_0077 0.30 -2.8 73,739 - Dsui_0077 0.30 -2.8 73,739 - Dsui_0077 0.30 -5.0 73,798 + Dsui_0077 0.35 -4.2 73,812 + Dsui_0077 0.36 -1.6 73,812 + Dsui_0077 0.36 -5.3
Or see this region's nucleotide sequence