Experiment: spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0055 and Dsui_0056 overlap by 14 nucleotides Dsui_0056 and Dsui_0057 overlap by 4 nucleotides
Dsui_0055: Dsui_0055 - glycosyltransferase, at 52,198 to 53,385
_0055
Dsui_0056: Dsui_0056 - glycosyl transferase, at 53,372 to 54,301
_0056
Dsui_0057: Dsui_0057 - glycosyltransferase, at 54,298 to 55,488
_0057
Position (kb) 53
54
55 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 52.399 kb on - strand, within Dsui_0055 at 52.578 kb on - strand, within Dsui_0055 at 52.583 kb on - strand, within Dsui_0055 at 52.607 kb on + strand, within Dsui_0055 at 52.685 kb on + strand, within Dsui_0055 at 52.826 kb on - strand, within Dsui_0055 at 52.865 kb on + strand, within Dsui_0055 at 52.870 kb on + strand at 52.958 kb on + strand at 52.966 kb on - strand, within Dsui_0055 at 52.994 kb on + strand, within Dsui_0055 at 52.994 kb on + strand at 52.994 kb on + strand, within Dsui_0055 at 53.002 kb on - strand, within Dsui_0055 at 53.160 kb on + strand at 53.173 kb on - strand, within Dsui_0055 at 53.214 kb on - strand, within Dsui_0055 at 53.223 kb on + strand, within Dsui_0055 at 53.250 kb on + strand, within Dsui_0055 at 53.296 kb on - strand at 53.499 kb on - strand, within Dsui_0056 at 53.557 kb on - strand, within Dsui_0056 at 53.593 kb on - strand, within Dsui_0056 at 53.637 kb on - strand, within Dsui_0056 at 53.650 kb on + strand at 53.712 kb on - strand, within Dsui_0056 at 53.770 kb on - strand, within Dsui_0056 at 53.790 kb on + strand, within Dsui_0056 at 53.829 kb on + strand, within Dsui_0056 at 53.952 kb on - strand, within Dsui_0056 at 54.093 kb on - strand, within Dsui_0056 at 54.093 kb on - strand, within Dsui_0056 at 54.279 kb on + strand at 54.340 kb on - strand at 54.430 kb on + strand, within Dsui_0057 at 54.430 kb on + strand, within Dsui_0057 at 54.430 kb on + strand, within Dsui_0057 at 54.457 kb on - strand, within Dsui_0057 at 54.557 kb on + strand, within Dsui_0057 at 54.585 kb on + strand, within Dsui_0057 at 54.845 kb on - strand, within Dsui_0057 at 54.848 kb on - strand, within Dsui_0057 at 54.873 kb on + strand, within Dsui_0057 at 54.873 kb on + strand, within Dsui_0057 at 54.881 kb on - strand, within Dsui_0057 at 54.910 kb on - strand, within Dsui_0057 at 54.961 kb on - strand, within Dsui_0057 at 55.089 kb on - strand, within Dsui_0057 at 55.227 kb on + strand, within Dsui_0057 at 55.235 kb on - strand, within Dsui_0057 at 55.262 kb on - strand, within Dsui_0057
Per-strain Table
Position Strand Gene LocusTag Fraction spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 52,399 - Dsui_0055 0.17 -1.3 52,578 - Dsui_0055 0.32 -1.5 52,583 - Dsui_0055 0.32 -1.4 52,607 + Dsui_0055 0.34 -1.8 52,685 + Dsui_0055 0.41 -2.2 52,826 - Dsui_0055 0.53 -1.8 52,865 + Dsui_0055 0.56 -2.2 52,870 + -1.3 52,958 + -1.6 52,966 - Dsui_0055 0.65 -0.9 52,994 + Dsui_0055 0.67 -2.6 52,994 + -0.5 52,994 + Dsui_0055 0.67 -2.2 53,002 - Dsui_0055 0.68 -3.8 53,160 + +0.5 53,173 - Dsui_0055 0.82 -1.5 53,214 - Dsui_0055 0.86 -2.0 53,223 + Dsui_0055 0.86 -1.8 53,250 + Dsui_0055 0.89 -3.0 53,296 - -2.5 53,499 - Dsui_0056 0.14 -1.9 53,557 - Dsui_0056 0.20 -4.1 53,593 - Dsui_0056 0.24 -3.0 53,637 - Dsui_0056 0.28 -2.0 53,650 + -1.0 53,712 - Dsui_0056 0.37 -4.3 53,770 - Dsui_0056 0.43 -1.4 53,790 + Dsui_0056 0.45 -1.6 53,829 + Dsui_0056 0.49 -2.5 53,952 - Dsui_0056 0.62 -1.4 54,093 - Dsui_0056 0.78 -3.5 54,093 - Dsui_0056 0.78 -3.1 54,279 + -1.0 54,340 - +0.7 54,430 + Dsui_0057 0.11 -1.3 54,430 + Dsui_0057 0.11 +1.5 54,430 + Dsui_0057 0.11 -2.2 54,457 - Dsui_0057 0.13 -2.4 54,557 + Dsui_0057 0.22 -4.0 54,585 + Dsui_0057 0.24 -2.9 54,845 - Dsui_0057 0.46 -2.3 54,848 - Dsui_0057 0.46 -2.0 54,873 + Dsui_0057 0.48 -3.0 54,873 + Dsui_0057 0.48 -4.2 54,881 - Dsui_0057 0.49 -2.0 54,910 - Dsui_0057 0.51 -2.6 54,961 - Dsui_0057 0.56 -2.3 55,089 - Dsui_0057 0.66 -1.9 55,227 + Dsui_0057 0.78 -3.1 55,235 - Dsui_0057 0.79 -2.0 55,262 - Dsui_0057 0.81 -2.1
Or see this region's nucleotide sequence