Experiment: spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0006 and Dsui_0007 are separated by 85 nucleotides Dsui_0007 and Dsui_0008 are separated by 312 nucleotides Dsui_0008 and Dsui_0009 are separated by 0 nucleotides Dsui_0009 and Dsui_0010 are separated by 107 nucleotides
Dsui_0006: Dsui_0006 - sulfur oxidation protein SoxZ, at 4,836 to 5,153
_0006
Dsui_0007: Dsui_0007 - cold shock protein, at 5,239 to 5,442
_0007
Dsui_0008: Dsui_0008 - hypothetical protein, at 5,755 to 6,072
_0008
Dsui_0009: Dsui_0009 - ATP-dependent Clp protease ATP-binding subunit clpA, at 6,073 to 8,328
_0009
Dsui_0010: Dsui_0010 - RraA family protein, at 8,436 to 8,927
_0010
Position (kb) 6
7
8
9 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1
2
3 at 5.077 kb on - strand at 5.123 kb on - strand at 5.123 kb on - strand at 5.164 kb on + strand at 5.172 kb on - strand at 5.172 kb on - strand at 5.172 kb on - strand at 5.173 kb on - strand at 5.237 kb on + strand at 5.237 kb on + strand at 5.237 kb on + strand at 5.244 kb on - strand at 5.244 kb on - strand at 5.244 kb on - strand at 5.297 kb on - strand, within Dsui_0007 at 5.359 kb on + strand at 5.372 kb on - strand, within Dsui_0007 at 5.396 kb on + strand, within Dsui_0007 at 5.443 kb on - strand at 5.492 kb on - strand at 5.547 kb on - strand at 5.569 kb on + strand at 5.587 kb on - strand at 5.600 kb on - strand at 5.616 kb on + strand at 5.651 kb on + strand at 5.651 kb on + strand at 5.651 kb on + strand at 5.659 kb on - strand at 5.659 kb on - strand at 5.659 kb on - strand at 5.659 kb on - strand at 5.710 kb on + strand at 5.710 kb on + strand at 5.710 kb on + strand at 5.710 kb on + strand at 5.710 kb on + strand at 5.718 kb on + strand at 5.718 kb on + strand at 5.718 kb on - strand at 5.718 kb on - strand at 5.726 kb on - strand at 5.726 kb on - strand at 5.730 kb on + strand at 5.730 kb on + strand at 5.730 kb on + strand at 5.730 kb on + strand at 5.738 kb on - strand at 5.740 kb on + strand at 5.748 kb on - strand at 5.750 kb on + strand at 5.751 kb on + strand at 5.751 kb on + strand at 5.759 kb on - strand at 5.759 kb on - strand at 5.776 kb on + strand at 5.776 kb on + strand at 5.776 kb on + strand at 5.781 kb on + strand at 5.784 kb on - strand at 5.786 kb on + strand at 5.786 kb on + strand at 5.786 kb on + strand at 5.786 kb on + strand at 5.786 kb on + strand at 5.786 kb on + strand at 5.794 kb on - strand at 5.794 kb on - strand, within Dsui_0008 at 5.807 kb on + strand, within Dsui_0008 at 5.807 kb on + strand, within Dsui_0008 at 5.814 kb on + strand, within Dsui_0008 at 5.815 kb on - strand, within Dsui_0008 at 5.827 kb on - strand, within Dsui_0008 at 5.831 kb on + strand, within Dsui_0008 at 5.839 kb on - strand, within Dsui_0008 at 5.866 kb on + strand, within Dsui_0008 at 5.866 kb on + strand, within Dsui_0008 at 5.874 kb on - strand, within Dsui_0008 at 5.936 kb on + strand, within Dsui_0008 at 5.972 kb on + strand, within Dsui_0008 at 5.974 kb on + strand, within Dsui_0008 at 5.975 kb on - strand, within Dsui_0008 at 5.975 kb on - strand, within Dsui_0008 at 5.980 kb on - strand, within Dsui_0008 at 5.985 kb on + strand, within Dsui_0008 at 5.996 kb on + strand, within Dsui_0008 at 5.996 kb on + strand, within Dsui_0008 at 5.996 kb on + strand, within Dsui_0008 at 5.996 kb on + strand, within Dsui_0008 at 6.002 kb on + strand, within Dsui_0008 at 6.007 kb on + strand, within Dsui_0008 at 6.043 kb on + strand at 6.053 kb on + strand at 6.269 kb on + strand at 6.277 kb on - strand at 6.277 kb on - strand at 6.318 kb on - strand at 6.422 kb on + strand, within Dsui_0009 at 6.555 kb on + strand, within Dsui_0009 at 6.576 kb on + strand, within Dsui_0009 at 6.612 kb on + strand, within Dsui_0009 at 6.662 kb on + strand, within Dsui_0009 at 6.670 kb on - strand, within Dsui_0009 at 6.724 kb on - strand, within Dsui_0009 at 6.750 kb on - strand, within Dsui_0009 at 6.799 kb on - strand, within Dsui_0009 at 6.852 kb on + strand, within Dsui_0009 at 6.864 kb on + strand, within Dsui_0009 at 6.864 kb on + strand, within Dsui_0009 at 6.876 kb on + strand, within Dsui_0009 at 6.884 kb on - strand, within Dsui_0009 at 6.891 kb on + strand, within Dsui_0009 at 6.973 kb on - strand, within Dsui_0009 at 6.982 kb on - strand, within Dsui_0009 at 6.984 kb on + strand, within Dsui_0009 at 6.984 kb on + strand, within Dsui_0009 at 7.019 kb on + strand, within Dsui_0009 at 7.123 kb on - strand, within Dsui_0009 at 7.127 kb on - strand, within Dsui_0009 at 7.147 kb on + strand, within Dsui_0009 at 7.195 kb on + strand, within Dsui_0009 at 7.383 kb on - strand, within Dsui_0009 at 7.609 kb on + strand, within Dsui_0009 at 7.612 kb on - strand, within Dsui_0009 at 7.617 kb on - strand, within Dsui_0009 at 7.617 kb on - strand, within Dsui_0009 at 7.675 kb on + strand, within Dsui_0009 at 7.713 kb on + strand, within Dsui_0009 at 7.734 kb on + strand, within Dsui_0009 at 7.734 kb on + strand, within Dsui_0009 at 7.742 kb on - strand at 7.979 kb on - strand, within Dsui_0009 at 8.105 kb on - strand at 8.126 kb on + strand at 8.157 kb on + strand at 8.269 kb on - strand at 8.331 kb on - strand at 8.335 kb on - strand at 8.441 kb on - strand at 8.443 kb on + strand at 8.449 kb on + strand at 8.449 kb on + strand at 8.449 kb on + strand at 8.449 kb on + strand at 8.449 kb on + strand at 8.457 kb on - strand at 8.457 kb on - strand at 8.457 kb on - strand at 8.457 kb on - strand at 8.457 kb on - strand at 8.457 kb on - strand at 8.457 kb on - strand at 8.485 kb on + strand at 8.485 kb on + strand at 8.521 kb on - strand, within Dsui_0010 at 8.533 kb on + strand, within Dsui_0010 at 8.562 kb on - strand, within Dsui_0010 at 8.601 kb on - strand, within Dsui_0010 at 8.601 kb on - strand, within Dsui_0010 at 8.606 kb on - strand, within Dsui_0010 at 8.618 kb on + strand, within Dsui_0010 at 8.626 kb on - strand, within Dsui_0010 at 8.626 kb on - strand, within Dsui_0010 at 8.764 kb on + strand, within Dsui_0010 at 8.781 kb on + strand, within Dsui_0010 at 8.894 kb on - strand at 8.991 kb on + strand at 9.010 kb on + strand at 9.070 kb on - strand at 9.089 kb on + strand at 9.103 kb on + strand at 9.279 kb on + strand at 9.279 kb on + strand at 9.281 kb on + strand at 9.287 kb on - strand at 9.291 kb on + strand at 9.291 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 5,077 - +1.2 5,123 - +1.5 5,123 - +0.1 5,164 + +0.5 5,172 - +2.9 5,172 - +0.5 5,172 - +0.5 5,173 - +0.5 5,237 + -0.8 5,237 + -3.4 5,237 + +0.5 5,244 - -1.4 5,244 - +0.4 5,244 - +0.1 5,297 - Dsui_0007 0.28 -1.3 5,359 + -1.3 5,372 - Dsui_0007 0.65 -1.6 5,396 + Dsui_0007 0.77 -2.2 5,443 - -2.4 5,492 - +1.2 5,547 - -2.6 5,569 + +1.5 5,587 - -2.2 5,600 - -2.3 5,616 + -1.5 5,651 + +1.5 5,651 + -2.0 5,651 + +0.7 5,659 - -0.3 5,659 - -2.3 5,659 - -0.2 5,659 - +0.8 5,710 + +0.4 5,710 + -0.1 5,710 + -0.6 5,710 + -0.0 5,710 + +0.8 5,718 + +0.2 5,718 + -0.2 5,718 - +0.1 5,718 - +1.3 5,726 - +1.6 5,726 - +0.9 5,730 + -0.1 5,730 + -0.5 5,730 + +0.1 5,730 + -1.2 5,738 - -0.3 5,740 + +0.7 5,748 - +0.0 5,750 + +1.6 5,751 + +1.9 5,751 + +0.6 5,759 - +2.5 5,759 - -0.5 5,776 + -1.6 5,776 + +0.9 5,776 + +0.6 5,781 + +0.9 5,784 - +0.3 5,786 + +0.6 5,786 + +1.2 5,786 + +0.8 5,786 + +1.1 5,786 + +0.1 5,786 + -0.6 5,794 - -0.9 5,794 - Dsui_0008 0.12 -0.0 5,807 + Dsui_0008 0.16 +0.4 5,807 + Dsui_0008 0.16 -0.6 5,814 + Dsui_0008 0.19 +0.7 5,815 - Dsui_0008 0.19 -0.6 5,827 - Dsui_0008 0.23 -0.8 5,831 + Dsui_0008 0.24 +0.1 5,839 - Dsui_0008 0.26 +0.4 5,866 + Dsui_0008 0.35 +0.1 5,866 + Dsui_0008 0.35 +0.1 5,874 - Dsui_0008 0.37 +0.4 5,936 + Dsui_0008 0.57 +0.1 5,972 + Dsui_0008 0.68 +0.1 5,974 + Dsui_0008 0.69 +0.4 5,975 - Dsui_0008 0.69 +0.8 5,975 - Dsui_0008 0.69 -0.0 5,980 - Dsui_0008 0.71 -3.2 5,985 + Dsui_0008 0.72 +0.7 5,996 + Dsui_0008 0.76 +0.8 5,996 + Dsui_0008 0.76 +0.2 5,996 + Dsui_0008 0.76 -0.4 5,996 + Dsui_0008 0.76 +0.2 6,002 + Dsui_0008 0.78 +0.8 6,007 + Dsui_0008 0.79 +0.5 6,043 + +0.4 6,053 + -0.3 6,269 + -2.2 6,277 - -4.3 6,277 - -2.5 6,318 - -1.3 6,422 + Dsui_0009 0.15 -4.4 6,555 + Dsui_0009 0.21 -3.6 6,576 + Dsui_0009 0.22 -2.5 6,612 + Dsui_0009 0.24 -6.2 6,662 + Dsui_0009 0.26 -3.2 6,670 - Dsui_0009 0.26 -6.3 6,724 - Dsui_0009 0.29 -2.7 6,750 - Dsui_0009 0.30 -5.4 6,799 - Dsui_0009 0.32 -5.0 6,852 + Dsui_0009 0.35 -5.4 6,864 + Dsui_0009 0.35 -4.8 6,864 + Dsui_0009 0.35 -3.9 6,876 + Dsui_0009 0.36 -2.2 6,884 - Dsui_0009 0.36 -3.6 6,891 + Dsui_0009 0.36 -5.2 6,973 - Dsui_0009 0.40 -3.2 6,982 - Dsui_0009 0.40 -7.6 6,984 + Dsui_0009 0.40 -4.6 6,984 + Dsui_0009 0.40 -4.4 7,019 + Dsui_0009 0.42 -5.1 7,123 - Dsui_0009 0.47 -4.5 7,127 - Dsui_0009 0.47 -3.3 7,147 + Dsui_0009 0.48 -3.0 7,195 + Dsui_0009 0.50 -2.5 7,383 - Dsui_0009 0.58 -1.8 7,609 + Dsui_0009 0.68 -3.1 7,612 - Dsui_0009 0.68 -1.9 7,617 - Dsui_0009 0.68 -4.9 7,617 - Dsui_0009 0.68 -5.3 7,675 + Dsui_0009 0.71 -5.7 7,713 + Dsui_0009 0.73 -3.7 7,734 + Dsui_0009 0.74 -3.7 7,734 + Dsui_0009 0.74 -3.3 7,742 - -2.5 7,979 - Dsui_0009 0.84 -5.0 8,105 - -2.9 8,126 + -4.0 8,157 + -3.6 8,269 - -2.6 8,331 - +0.8 8,335 - +0.6 8,441 - +1.0 8,443 + +1.0 8,449 + +1.1 8,449 + +0.9 8,449 + +0.8 8,449 + +0.3 8,449 + -0.5 8,457 - -1.3 8,457 - +1.1 8,457 - -1.3 8,457 - +1.5 8,457 - +0.6 8,457 - -0.6 8,457 - +0.7 8,485 + +2.2 8,485 + +2.4 8,521 - Dsui_0010 0.17 +0.6 8,533 + Dsui_0010 0.20 +0.9 8,562 - Dsui_0010 0.26 +0.7 8,601 - Dsui_0010 0.34 +1.8 8,601 - Dsui_0010 0.34 +0.2 8,606 - Dsui_0010 0.35 +2.0 8,618 + Dsui_0010 0.37 +0.9 8,626 - Dsui_0010 0.39 +0.3 8,626 - Dsui_0010 0.39 +0.8 8,764 + Dsui_0010 0.67 -0.3 8,781 + Dsui_0010 0.70 +0.7 8,894 - +0.7 8,991 + +1.5 9,010 + -1.6 9,070 - +1.8 9,089 + +0.3 9,103 + +0.6 9,279 + +1.0 9,279 + +0.4 9,281 + +0.7 9,287 - -0.5 9,291 + +0.4 9,291 + +1.7
Or see this region's nucleotide sequence