Experiment: spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0004 and Dsui_0005 are separated by 32 nucleotides Dsui_0005 and Dsui_0006 are separated by 9 nucleotides Dsui_0006 and Dsui_0007 are separated by 85 nucleotides Dsui_0007 and Dsui_0008 are separated by 312 nucleotides Dsui_0008 and Dsui_0009 are separated by 0 nucleotides
Dsui_0004: Dsui_0004 - molybdenum cofactor biosynthesis protein MoaC, at 3,829 to 4,323
_0004
Dsui_0005: Dsui_0005 - putative secreted protein, at 4,356 to 4,826
_0005
Dsui_0006: Dsui_0006 - sulfur oxidation protein SoxZ, at 4,836 to 5,153
_0006
Dsui_0007: Dsui_0007 - cold shock protein, at 5,239 to 5,442
_0007
Dsui_0008: Dsui_0008 - hypothetical protein, at 5,755 to 6,072
_0008
Dsui_0009: Dsui_0009 - ATP-dependent Clp protease ATP-binding subunit clpA, at 6,073 to 8,328
_0009
Position (kb) 5
6 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1
2
3 at 4.241 kb on - strand at 4.260 kb on + strand, within Dsui_0004 at 4.426 kb on - strand, within Dsui_0005 at 4.426 kb on - strand at 4.504 kb on - strand, within Dsui_0005 at 4.522 kb on - strand, within Dsui_0005 at 4.630 kb on + strand, within Dsui_0005 at 4.631 kb on - strand, within Dsui_0005 at 4.695 kb on + strand, within Dsui_0005 at 4.705 kb on + strand, within Dsui_0005 at 4.705 kb on + strand, within Dsui_0005 at 4.705 kb on + strand, within Dsui_0005 at 4.705 kb on + strand, within Dsui_0005 at 4.713 kb on - strand, within Dsui_0005 at 4.725 kb on - strand, within Dsui_0005 at 4.742 kb on + strand, within Dsui_0005 at 4.750 kb on - strand, within Dsui_0005 at 4.750 kb on - strand, within Dsui_0005 at 4.750 kb on - strand, within Dsui_0005 at 4.750 kb on - strand, within Dsui_0005 at 4.762 kb on - strand at 4.861 kb on + strand at 4.887 kb on + strand, within Dsui_0006 at 4.893 kb on - strand, within Dsui_0006 at 4.910 kb on + strand, within Dsui_0006 at 5.002 kb on + strand, within Dsui_0006 at 5.002 kb on - strand, within Dsui_0006 at 5.007 kb on + strand, within Dsui_0006 at 5.007 kb on + strand, within Dsui_0006 at 5.026 kb on - strand, within Dsui_0006 at 5.077 kb on - strand at 5.123 kb on - strand at 5.123 kb on - strand at 5.164 kb on + strand at 5.172 kb on - strand at 5.172 kb on - strand at 5.172 kb on - strand at 5.173 kb on - strand at 5.237 kb on + strand at 5.237 kb on + strand at 5.237 kb on + strand at 5.244 kb on - strand at 5.244 kb on - strand at 5.244 kb on - strand at 5.297 kb on - strand, within Dsui_0007 at 5.359 kb on + strand at 5.372 kb on - strand, within Dsui_0007 at 5.396 kb on + strand, within Dsui_0007 at 5.443 kb on - strand at 5.492 kb on - strand at 5.547 kb on - strand at 5.569 kb on + strand at 5.587 kb on - strand at 5.600 kb on - strand at 5.616 kb on + strand at 5.651 kb on + strand at 5.651 kb on + strand at 5.651 kb on + strand at 5.659 kb on - strand at 5.659 kb on - strand at 5.659 kb on - strand at 5.659 kb on - strand at 5.710 kb on + strand at 5.710 kb on + strand at 5.710 kb on + strand at 5.710 kb on + strand at 5.710 kb on + strand at 5.718 kb on + strand at 5.718 kb on + strand at 5.718 kb on - strand at 5.718 kb on - strand at 5.726 kb on - strand at 5.726 kb on - strand at 5.730 kb on + strand at 5.730 kb on + strand at 5.730 kb on + strand at 5.730 kb on + strand at 5.738 kb on - strand at 5.740 kb on + strand at 5.748 kb on - strand at 5.750 kb on + strand at 5.751 kb on + strand at 5.751 kb on + strand at 5.759 kb on - strand at 5.759 kb on - strand at 5.776 kb on + strand at 5.776 kb on + strand at 5.776 kb on + strand at 5.781 kb on + strand at 5.784 kb on - strand at 5.786 kb on + strand at 5.786 kb on + strand at 5.786 kb on + strand at 5.786 kb on + strand at 5.786 kb on + strand at 5.786 kb on + strand at 5.794 kb on - strand at 5.794 kb on - strand, within Dsui_0008 at 5.807 kb on + strand, within Dsui_0008 at 5.807 kb on + strand, within Dsui_0008 at 5.814 kb on + strand, within Dsui_0008 at 5.815 kb on - strand, within Dsui_0008 at 5.827 kb on - strand, within Dsui_0008 at 5.831 kb on + strand, within Dsui_0008 at 5.839 kb on - strand, within Dsui_0008 at 5.866 kb on + strand, within Dsui_0008 at 5.866 kb on + strand, within Dsui_0008 at 5.874 kb on - strand, within Dsui_0008 at 5.936 kb on + strand, within Dsui_0008 at 5.972 kb on + strand, within Dsui_0008 at 5.974 kb on + strand, within Dsui_0008 at 5.975 kb on - strand, within Dsui_0008 at 5.975 kb on - strand, within Dsui_0008 at 5.980 kb on - strand, within Dsui_0008 at 5.985 kb on + strand, within Dsui_0008 at 5.996 kb on + strand, within Dsui_0008 at 5.996 kb on + strand, within Dsui_0008 at 5.996 kb on + strand, within Dsui_0008 at 5.996 kb on + strand, within Dsui_0008 at 6.002 kb on + strand, within Dsui_0008 at 6.007 kb on + strand, within Dsui_0008 at 6.043 kb on + strand at 6.053 kb on + strand at 6.269 kb on + strand at 6.277 kb on - strand at 6.277 kb on - strand at 6.318 kb on - strand at 6.422 kb on + strand, within Dsui_0009
Per-strain Table
Position Strand Gene LocusTag Fraction spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 4,241 - -3.0 4,260 + Dsui_0004 0.87 -7.1 4,426 - Dsui_0005 0.15 -0.3 4,426 - -1.4 4,504 - Dsui_0005 0.31 +2.5 4,522 - Dsui_0005 0.35 +1.1 4,630 + Dsui_0005 0.58 +1.4 4,631 - Dsui_0005 0.58 +2.1 4,695 + Dsui_0005 0.72 +1.5 4,705 + Dsui_0005 0.74 +1.1 4,705 + Dsui_0005 0.74 +1.1 4,705 + Dsui_0005 0.74 +1.7 4,705 + Dsui_0005 0.74 +1.7 4,713 - Dsui_0005 0.76 +1.4 4,725 - Dsui_0005 0.78 -0.6 4,742 + Dsui_0005 0.82 +1.7 4,750 - Dsui_0005 0.84 +0.5 4,750 - Dsui_0005 0.84 +0.1 4,750 - Dsui_0005 0.84 -0.8 4,750 - Dsui_0005 0.84 -0.0 4,762 - +0.7 4,861 + +1.6 4,887 + Dsui_0006 0.16 +0.5 4,893 - Dsui_0006 0.18 +0.7 4,910 + Dsui_0006 0.23 +0.7 5,002 + Dsui_0006 0.52 +1.3 5,002 - Dsui_0006 0.52 +0.8 5,007 + Dsui_0006 0.54 +1.4 5,007 + Dsui_0006 0.54 +1.4 5,026 - Dsui_0006 0.60 +0.8 5,077 - +1.2 5,123 - +1.5 5,123 - +0.1 5,164 + +0.5 5,172 - +2.9 5,172 - +0.5 5,172 - +0.5 5,173 - +0.5 5,237 + -0.8 5,237 + -3.4 5,237 + +0.5 5,244 - -1.4 5,244 - +0.4 5,244 - +0.1 5,297 - Dsui_0007 0.28 -1.3 5,359 + -1.3 5,372 - Dsui_0007 0.65 -1.6 5,396 + Dsui_0007 0.77 -2.2 5,443 - -2.4 5,492 - +1.2 5,547 - -2.6 5,569 + +1.5 5,587 - -2.2 5,600 - -2.3 5,616 + -1.5 5,651 + +1.5 5,651 + -2.0 5,651 + +0.7 5,659 - -0.3 5,659 - -2.3 5,659 - -0.2 5,659 - +0.8 5,710 + +0.4 5,710 + -0.1 5,710 + -0.6 5,710 + -0.0 5,710 + +0.8 5,718 + +0.2 5,718 + -0.2 5,718 - +0.1 5,718 - +1.3 5,726 - +1.6 5,726 - +0.9 5,730 + -0.1 5,730 + -0.5 5,730 + +0.1 5,730 + -1.2 5,738 - -0.3 5,740 + +0.7 5,748 - +0.0 5,750 + +1.6 5,751 + +1.9 5,751 + +0.6 5,759 - +2.5 5,759 - -0.5 5,776 + -1.6 5,776 + +0.9 5,776 + +0.6 5,781 + +0.9 5,784 - +0.3 5,786 + +0.6 5,786 + +1.2 5,786 + +0.8 5,786 + +1.1 5,786 + +0.1 5,786 + -0.6 5,794 - -0.9 5,794 - Dsui_0008 0.12 -0.0 5,807 + Dsui_0008 0.16 +0.4 5,807 + Dsui_0008 0.16 -0.6 5,814 + Dsui_0008 0.19 +0.7 5,815 - Dsui_0008 0.19 -0.6 5,827 - Dsui_0008 0.23 -0.8 5,831 + Dsui_0008 0.24 +0.1 5,839 - Dsui_0008 0.26 +0.4 5,866 + Dsui_0008 0.35 +0.1 5,866 + Dsui_0008 0.35 +0.1 5,874 - Dsui_0008 0.37 +0.4 5,936 + Dsui_0008 0.57 +0.1 5,972 + Dsui_0008 0.68 +0.1 5,974 + Dsui_0008 0.69 +0.4 5,975 - Dsui_0008 0.69 +0.8 5,975 - Dsui_0008 0.69 -0.0 5,980 - Dsui_0008 0.71 -3.2 5,985 + Dsui_0008 0.72 +0.7 5,996 + Dsui_0008 0.76 +0.8 5,996 + Dsui_0008 0.76 +0.2 5,996 + Dsui_0008 0.76 -0.4 5,996 + Dsui_0008 0.76 +0.2 6,002 + Dsui_0008 0.78 +0.8 6,007 + Dsui_0008 0.79 +0.5 6,043 + +0.4 6,053 + -0.3 6,269 + -2.2 6,277 - -4.3 6,277 - -2.5 6,318 - -1.3 6,422 + Dsui_0009 0.15 -4.4
Or see this region's nucleotide sequence