Experiment: ICP3_phage_4.1_MOI_rep2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_16120 and CSW01_16125 are separated by 192 nucleotides CSW01_16125 and CSW01_16130 are separated by 88 nucleotides CSW01_16130 and CSW01_16135 overlap by 7 nucleotides CSW01_16135 and CSW01_16140 overlap by 49 nucleotides CSW01_16140 and CSW01_16145 are separated by 43 nucleotides CSW01_16145 and CSW01_16150 are separated by 88 nucleotides CSW01_16150 and CSW01_16155 are separated by 147 nucleotides
CSW01_16120: CSW01_16120 - N-acetyltransferase, at 373,878 to 374,312
_16120
CSW01_16125: CSW01_16125 - hypothetical protein, at 374,505 to 374,762
_16125
CSW01_16130: CSW01_16130 - acetyltransferase, at 374,851 to 375,012
_16130
CSW01_16135: CSW01_16135 - general secretion pathway protein GspH, at 375,006 to 375,146
_16135
CSW01_16140: CSW01_16140 - DUF645 domain-containing protein, at 375,098 to 375,268
_16140
CSW01_16145: CSW01_16145 - hypothetical protein, at 375,312 to 375,407
_16145
CSW01_16150: CSW01_16150 - hypothetical protein, at 375,496 to 376,116
_16150
CSW01_16155: CSW01_16155 - lipocalin, at 376,264 to 376,779
_16155
Position (kb) 375
376 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 374.244 kb on - strand, within CSW01_16120 at 374.276 kb on + strand at 374.276 kb on + strand at 374.549 kb on - strand, within CSW01_16125 at 374.564 kb on - strand, within CSW01_16125 at 374.564 kb on - strand, within CSW01_16125 at 374.627 kb on - strand, within CSW01_16125 at 374.627 kb on - strand, within CSW01_16125 at 374.704 kb on + strand, within CSW01_16125 at 374.717 kb on - strand, within CSW01_16125 at 374.991 kb on - strand, within CSW01_16130 at 375.197 kb on - strand, within CSW01_16140 at 375.197 kb on - strand, within CSW01_16140 at 375.197 kb on - strand, within CSW01_16140 at 375.439 kb on - strand at 375.571 kb on + strand, within CSW01_16150 at 375.579 kb on - strand, within CSW01_16150 at 375.579 kb on - strand, within CSW01_16150 at 375.579 kb on - strand, within CSW01_16150 at 375.586 kb on + strand, within CSW01_16150 at 375.595 kb on + strand, within CSW01_16150 at 375.697 kb on - strand, within CSW01_16150 at 375.725 kb on - strand, within CSW01_16150 at 375.725 kb on - strand, within CSW01_16150 at 375.753 kb on - strand, within CSW01_16150 at 375.858 kb on - strand, within CSW01_16150 at 375.858 kb on - strand, within CSW01_16150 at 375.988 kb on + strand, within CSW01_16150 at 375.988 kb on + strand, within CSW01_16150 at 376.022 kb on + strand, within CSW01_16150 at 376.022 kb on + strand, within CSW01_16150 at 376.084 kb on - strand at 376.084 kb on - strand at 376.142 kb on - strand at 376.142 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ICP3_phage_4.1_MOI_rep2 remove 374,244 - CSW01_16120 0.84 +2.0 374,276 + -1.4 374,276 + -3.7 374,549 - CSW01_16125 0.17 -0.0 374,564 - CSW01_16125 0.23 -0.7 374,564 - CSW01_16125 0.23 +1.4 374,627 - CSW01_16125 0.47 +1.6 374,627 - CSW01_16125 0.47 +0.5 374,704 + CSW01_16125 0.77 -2.2 374,717 - CSW01_16125 0.82 -2.2 374,991 - CSW01_16130 0.86 -1.5 375,197 - CSW01_16140 0.58 +1.3 375,197 - CSW01_16140 0.58 +0.2 375,197 - CSW01_16140 0.58 +0.8 375,439 - +1.7 375,571 + CSW01_16150 0.12 -1.2 375,579 - CSW01_16150 0.13 -0.0 375,579 - CSW01_16150 0.13 -3.5 375,579 - CSW01_16150 0.13 -0.3 375,586 + CSW01_16150 0.14 -1.5 375,595 + CSW01_16150 0.16 +0.3 375,697 - CSW01_16150 0.32 -1.0 375,725 - CSW01_16150 0.37 -2.9 375,725 - CSW01_16150 0.37 +0.5 375,753 - CSW01_16150 0.41 +1.9 375,858 - CSW01_16150 0.58 +0.1 375,858 - CSW01_16150 0.58 -0.9 375,988 + CSW01_16150 0.79 -1.4 375,988 + CSW01_16150 0.79 +1.7 376,022 + CSW01_16150 0.85 -0.9 376,022 + CSW01_16150 0.85 +0.4 376,084 - +1.4 376,084 - -3.9 376,142 - -2.4 376,142 - -0.2
Or see this region's nucleotide sequence