Experiment: ICP3_phage_4.1_MOI_rep2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_14670 and CSW01_14675 are separated by 125 nucleotides CSW01_14675 and CSW01_14680 are separated by 45 nucleotides CSW01_14680 and CSW01_14685 overlap by 4 nucleotides CSW01_14685 and CSW01_14690 are separated by 139 nucleotides CSW01_14690 and CSW01_14695 are separated by 52 nucleotides
CSW01_14670: CSW01_14670 - transcriptional repressor PurR, at 146,644 to 147,648
_14670
CSW01_14675: CSW01_14675 - nicotinamide mononucleotide transporter, at 147,774 to 148,340
_14675
CSW01_14680: CSW01_14680 - aminotransferase, at 148,386 to 148,508
_14680
CSW01_14685: CSW01_14685 - uridine phosphorylase, at 148,505 to 149,236
_14685
CSW01_14690: CSW01_14690 - Crp/Fnr family transcriptional regulator, at 149,376 to 150,056
_14690
CSW01_14695: CSW01_14695 - glycerophosphodiester phosphodiesterase, at 150,109 to 151,176
_14695
Position (kb) 148
149
150 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 147.553 kb on - strand at 147.563 kb on - strand at 147.599 kb on + strand at 147.611 kb on + strand at 147.619 kb on + strand at 147.629 kb on - strand at 147.629 kb on - strand at 147.700 kb on + strand at 147.700 kb on + strand at 147.756 kb on - strand at 147.801 kb on + strand at 147.802 kb on + strand at 147.845 kb on + strand, within CSW01_14675 at 147.850 kb on + strand, within CSW01_14675 at 148.125 kb on - strand, within CSW01_14675 at 148.140 kb on - strand, within CSW01_14675 at 148.197 kb on + strand, within CSW01_14675 at 148.216 kb on - strand, within CSW01_14675 at 148.218 kb on + strand, within CSW01_14675 at 148.218 kb on + strand, within CSW01_14675 at 148.221 kb on - strand, within CSW01_14675 at 148.226 kb on - strand, within CSW01_14675 at 148.236 kb on - strand, within CSW01_14675 at 148.281 kb on - strand, within CSW01_14675 at 148.352 kb on - strand at 148.466 kb on + strand, within CSW01_14680 at 148.548 kb on + strand at 148.548 kb on + strand at 148.548 kb on + strand at 148.568 kb on - strand at 148.605 kb on + strand, within CSW01_14685 at 148.605 kb on + strand, within CSW01_14685 at 148.608 kb on - strand, within CSW01_14685 at 148.688 kb on + strand, within CSW01_14685 at 148.756 kb on - strand, within CSW01_14685 at 148.807 kb on - strand, within CSW01_14685 at 148.850 kb on - strand, within CSW01_14685 at 148.850 kb on - strand, within CSW01_14685 at 148.850 kb on - strand, within CSW01_14685 at 148.907 kb on - strand, within CSW01_14685 at 148.920 kb on - strand, within CSW01_14685 at 148.961 kb on + strand, within CSW01_14685 at 148.969 kb on + strand, within CSW01_14685 at 149.043 kb on + strand, within CSW01_14685 at 149.051 kb on - strand, within CSW01_14685 at 149.053 kb on + strand, within CSW01_14685 at 149.053 kb on + strand, within CSW01_14685 at 149.061 kb on - strand, within CSW01_14685 at 149.076 kb on + strand, within CSW01_14685 at 149.134 kb on - strand, within CSW01_14685 at 149.157 kb on - strand, within CSW01_14685 at 149.176 kb on + strand at 149.236 kb on + strand at 149.236 kb on + strand at 149.236 kb on + strand at 149.243 kb on - strand at 149.290 kb on - strand at 149.342 kb on + strand at 149.607 kb on - strand, within CSW01_14690 at 149.663 kb on + strand, within CSW01_14690 at 149.726 kb on - strand, within CSW01_14690 at 149.736 kb on - strand, within CSW01_14690 at 149.753 kb on - strand, within CSW01_14690 at 149.780 kb on + strand, within CSW01_14690 at 149.788 kb on - strand, within CSW01_14690 at 149.801 kb on + strand, within CSW01_14690 at 149.890 kb on + strand, within CSW01_14690 at 149.908 kb on - strand, within CSW01_14690 at 150.054 kb on - strand at 150.198 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ICP3_phage_4.1_MOI_rep2 remove 147,553 - -0.7 147,563 - +1.4 147,599 + -1.3 147,611 + -0.9 147,619 + -2.6 147,629 - +1.1 147,629 - -2.5 147,700 + -1.2 147,700 + -0.4 147,756 - +1.0 147,801 + +0.5 147,802 + -1.0 147,845 + CSW01_14675 0.13 +1.0 147,850 + CSW01_14675 0.13 -2.4 148,125 - CSW01_14675 0.62 -2.0 148,140 - CSW01_14675 0.65 -1.0 148,197 + CSW01_14675 0.75 +0.2 148,216 - CSW01_14675 0.78 -0.5 148,218 + CSW01_14675 0.78 -0.6 148,218 + CSW01_14675 0.78 -2.4 148,221 - CSW01_14675 0.79 +0.7 148,226 - CSW01_14675 0.80 +1.5 148,236 - CSW01_14675 0.81 -0.0 148,281 - CSW01_14675 0.89 -0.1 148,352 - -3.4 148,466 + CSW01_14680 0.65 -0.3 148,548 + -2.5 148,548 + -2.0 148,548 + -0.3 148,568 - -2.5 148,605 + CSW01_14685 0.14 -0.8 148,605 + CSW01_14685 0.14 -1.2 148,608 - CSW01_14685 0.14 -2.6 148,688 + CSW01_14685 0.25 +1.6 148,756 - CSW01_14685 0.34 +0.5 148,807 - CSW01_14685 0.41 +0.9 148,850 - CSW01_14685 0.47 -2.7 148,850 - CSW01_14685 0.47 -0.5 148,850 - CSW01_14685 0.47 +1.3 148,907 - CSW01_14685 0.55 -0.6 148,920 - CSW01_14685 0.57 -0.9 148,961 + CSW01_14685 0.62 -1.6 148,969 + CSW01_14685 0.63 -2.2 149,043 + CSW01_14685 0.73 -1.4 149,051 - CSW01_14685 0.75 +2.0 149,053 + CSW01_14685 0.75 -0.3 149,053 + CSW01_14685 0.75 +1.4 149,061 - CSW01_14685 0.76 -0.7 149,076 + CSW01_14685 0.78 -2.7 149,134 - CSW01_14685 0.86 +1.0 149,157 - CSW01_14685 0.89 -2.7 149,176 + +1.0 149,236 + -0.3 149,236 + +0.9 149,236 + +1.3 149,243 - +0.6 149,290 - +1.1 149,342 + -0.1 149,607 - CSW01_14690 0.34 -1.0 149,663 + CSW01_14690 0.42 +0.7 149,726 - CSW01_14690 0.51 -0.4 149,736 - CSW01_14690 0.53 -0.5 149,753 - CSW01_14690 0.55 +0.6 149,780 + CSW01_14690 0.59 +0.1 149,788 - CSW01_14690 0.60 -0.4 149,801 + CSW01_14690 0.62 -0.4 149,890 + CSW01_14690 0.75 -0.6 149,908 - CSW01_14690 0.78 +0.4 150,054 - -1.5 150,198 + -1.5
Or see this region's nucleotide sequence