Experiment: ICP3_phage_4.1_MOI_rep2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_11740 and CSW01_11745 are separated by 69 nucleotides CSW01_11745 and CSW01_11750 are separated by 4 nucleotides CSW01_11750 and CSW01_11755 are separated by 97 nucleotides
CSW01_11740: CSW01_11740 - cysteine desulfurase CsdA, at 2,491,651 to 2,492,862
_11740
CSW01_11745: CSW01_11745 - cysteine desulfurase sulfur acceptor subunit CsdE, at 2,492,932 to 2,493,366
_11745
CSW01_11750: CSW01_11750 - tRNA cyclic N6-threonylcarbamoyladenosine(37) synthase TcdA, at 2,493,371 to 2,494,186
_11750
CSW01_11755: CSW01_11755 - murein transglycosylase, at 2,494,284 to 2,495,390
_11755
Position (kb)
2492
2493
2494 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 2491.936 kb on + strand, within CSW01_11740 at 2492.003 kb on + strand, within CSW01_11740 at 2492.152 kb on + strand, within CSW01_11740 at 2492.201 kb on - strand, within CSW01_11740 at 2492.218 kb on + strand, within CSW01_11740 at 2492.273 kb on + strand, within CSW01_11740 at 2492.336 kb on - strand, within CSW01_11740 at 2492.350 kb on - strand, within CSW01_11740 at 2492.355 kb on - strand, within CSW01_11740 at 2492.429 kb on - strand, within CSW01_11740 at 2492.429 kb on - strand, within CSW01_11740 at 2492.435 kb on + strand, within CSW01_11740 at 2492.440 kb on + strand, within CSW01_11740 at 2492.443 kb on - strand, within CSW01_11740 at 2492.500 kb on + strand, within CSW01_11740 at 2492.500 kb on + strand, within CSW01_11740 at 2492.508 kb on - strand, within CSW01_11740 at 2492.508 kb on - strand, within CSW01_11740 at 2492.508 kb on - strand, within CSW01_11740 at 2492.508 kb on - strand, within CSW01_11740 at 2492.513 kb on + strand, within CSW01_11740 at 2492.639 kb on + strand, within CSW01_11740 at 2492.644 kb on + strand, within CSW01_11740 at 2492.644 kb on + strand, within CSW01_11740 at 2492.644 kb on + strand, within CSW01_11740 at 2492.644 kb on + strand, within CSW01_11740 at 2492.644 kb on + strand, within CSW01_11740 at 2492.644 kb on + strand, within CSW01_11740 at 2492.644 kb on + strand, within CSW01_11740 at 2492.652 kb on - strand, within CSW01_11740 at 2492.652 kb on - strand, within CSW01_11740 at 2492.682 kb on - strand, within CSW01_11740 at 2492.794 kb on + strand at 2492.794 kb on + strand at 2492.794 kb on + strand at 2492.794 kb on + strand at 2492.794 kb on + strand at 2492.794 kb on + strand at 2492.794 kb on + strand at 2492.794 kb on + strand at 2492.794 kb on + strand at 2492.794 kb on + strand at 2492.802 kb on - strand at 2492.802 kb on - strand at 2492.802 kb on - strand at 2492.802 kb on - strand at 2492.802 kb on - strand at 2492.802 kb on - strand at 2492.802 kb on - strand at 2492.802 kb on - strand at 2492.804 kb on + strand at 2492.820 kb on - strand at 2492.867 kb on + strand at 2492.947 kb on + strand at 2492.955 kb on - strand at 2493.036 kb on - strand, within CSW01_11745 at 2493.115 kb on - strand, within CSW01_11745 at 2493.120 kb on + strand, within CSW01_11745 at 2493.335 kb on + strand at 2493.361 kb on + strand at 2493.369 kb on + strand at 2493.438 kb on - strand at 2493.600 kb on + strand, within CSW01_11750 at 2493.694 kb on - strand, within CSW01_11750 at 2493.704 kb on - strand, within CSW01_11750 at 2493.725 kb on - strand, within CSW01_11750 at 2493.779 kb on - strand, within CSW01_11750 at 2493.873 kb on + strand, within CSW01_11750 at 2493.885 kb on + strand, within CSW01_11750 at 2493.885 kb on + strand, within CSW01_11750 at 2493.903 kb on - strand, within CSW01_11750 at 2493.929 kb on - strand, within CSW01_11750 at 2494.020 kb on - strand, within CSW01_11750 at 2494.034 kb on + strand, within CSW01_11750 at 2494.042 kb on - strand, within CSW01_11750 at 2494.053 kb on - strand, within CSW01_11750 at 2494.063 kb on - strand, within CSW01_11750 at 2494.200 kb on - strand at 2494.330 kb on - strand at 2494.357 kb on + strand at 2494.357 kb on + strand at 2494.359 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ICP3_phage_4.1_MOI_rep2 remove 2,491,936 + CSW01_11740 0.24 -2.5 2,492,003 + CSW01_11740 0.29 -0.4 2,492,152 + CSW01_11740 0.41 -1.7 2,492,201 - CSW01_11740 0.45 -1.2 2,492,218 + CSW01_11740 0.47 -1.3 2,492,273 + CSW01_11740 0.51 +0.0 2,492,336 - CSW01_11740 0.57 -3.4 2,492,350 - CSW01_11740 0.58 +0.2 2,492,355 - CSW01_11740 0.58 -1.8 2,492,429 - CSW01_11740 0.64 -2.2 2,492,429 - CSW01_11740 0.64 -3.1 2,492,435 + CSW01_11740 0.65 -5.3 2,492,440 + CSW01_11740 0.65 -2.9 2,492,443 - CSW01_11740 0.65 -0.7 2,492,500 + CSW01_11740 0.70 -0.8 2,492,500 + CSW01_11740 0.70 +0.8 2,492,508 - CSW01_11740 0.71 -2.6 2,492,508 - CSW01_11740 0.71 -1.6 2,492,508 - CSW01_11740 0.71 -1.3 2,492,508 - CSW01_11740 0.71 -1.6 2,492,513 + CSW01_11740 0.71 -0.3 2,492,639 + CSW01_11740 0.82 -1.8 2,492,644 + CSW01_11740 0.82 -2.2 2,492,644 + CSW01_11740 0.82 +3.4 2,492,644 + CSW01_11740 0.82 -0.7 2,492,644 + CSW01_11740 0.82 -1.1 2,492,644 + CSW01_11740 0.82 +0.3 2,492,644 + CSW01_11740 0.82 -2.6 2,492,644 + CSW01_11740 0.82 -1.0 2,492,652 - CSW01_11740 0.83 +1.8 2,492,652 - CSW01_11740 0.83 -1.7 2,492,682 - CSW01_11740 0.85 +0.4 2,492,794 + -2.2 2,492,794 + +0.9 2,492,794 + -1.6 2,492,794 + +2.2 2,492,794 + -0.0 2,492,794 + -0.7 2,492,794 + +0.3 2,492,794 + -3.0 2,492,794 + -4.2 2,492,794 + +0.3 2,492,802 - +0.2 2,492,802 - -0.2 2,492,802 - -2.3 2,492,802 - +0.8 2,492,802 - -3.5 2,492,802 - +0.9 2,492,802 - -3.2 2,492,802 - -2.6 2,492,804 + -2.4 2,492,820 - +0.1 2,492,867 + -0.4 2,492,947 + +0.6 2,492,955 - -3.5 2,493,036 - CSW01_11745 0.24 -1.4 2,493,115 - CSW01_11745 0.42 -1.3 2,493,120 + CSW01_11745 0.43 -0.0 2,493,335 + +0.4 2,493,361 + +2.5 2,493,369 + -0.4 2,493,438 - -2.2 2,493,600 + CSW01_11750 0.28 -0.7 2,493,694 - CSW01_11750 0.40 -3.0 2,493,704 - CSW01_11750 0.41 +0.5 2,493,725 - CSW01_11750 0.43 -1.3 2,493,779 - CSW01_11750 0.50 +1.3 2,493,873 + CSW01_11750 0.62 -0.3 2,493,885 + CSW01_11750 0.63 +1.9 2,493,885 + CSW01_11750 0.63 -5.2 2,493,903 - CSW01_11750 0.65 -3.8 2,493,929 - CSW01_11750 0.68 -0.3 2,494,020 - CSW01_11750 0.80 -0.3 2,494,034 + CSW01_11750 0.81 -0.6 2,494,042 - CSW01_11750 0.82 -1.2 2,494,053 - CSW01_11750 0.84 +1.7 2,494,063 - CSW01_11750 0.85 +0.6 2,494,200 - +0.9 2,494,330 - +0.5 2,494,357 + -1.0 2,494,357 + -2.1 2,494,359 + -0.2
Or see this region's nucleotide sequence