Experiment: ICP3_phage_4.1_MOI_rep2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_08915 and CSW01_08920 are separated by 7 nucleotides CSW01_08920 and CSW01_08925 are separated by 50 nucleotides CSW01_08925 and CSW01_08930 are separated by 30 nucleotides CSW01_08930 and CSW01_08935 overlap by 110 nucleotides CSW01_08935 and CSW01_08940 overlap by 13 nucleotides
CSW01_08915: CSW01_08915 - ABC transporter permease, at 1,925,892 to 1,927,175
_08915
CSW01_08920: CSW01_08920 - C4-dicarboxylate ABC transporter permease, at 1,927,183 to 1,927,704
_08920
CSW01_08925: CSW01_08925 - sialic acid-binding protein, at 1,927,755 to 1,928,720
_08925
CSW01_08930: CSW01_08930 - hypothetical protein, at 1,928,751 to 1,929,044
_08930
CSW01_08935: CSW01_08935 - N-acetylmannosamine-6-phosphate 2-epimerase, at 1,928,935 to 1,929,657
_08935
CSW01_08940: CSW01_08940 - N-acetylmannosamine kinase, at 1,929,645 to 1,930,508
_08940
Position (kb)
1927
1928
1929 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1926.784 kb on + strand, within CSW01_08915 at 1926.832 kb on - strand, within CSW01_08915 at 1926.832 kb on - strand, within CSW01_08915 at 1926.843 kb on + strand, within CSW01_08915 at 1926.926 kb on + strand, within CSW01_08915 at 1927.035 kb on + strand, within CSW01_08915 at 1927.271 kb on + strand, within CSW01_08920 at 1927.306 kb on + strand, within CSW01_08920 at 1927.340 kb on - strand, within CSW01_08920 at 1927.366 kb on - strand, within CSW01_08920 at 1927.374 kb on + strand, within CSW01_08920 at 1927.374 kb on + strand, within CSW01_08920 at 1927.400 kb on - strand, within CSW01_08920 at 1927.434 kb on + strand, within CSW01_08920 at 1927.525 kb on - strand, within CSW01_08920 at 1927.560 kb on + strand, within CSW01_08920 at 1927.568 kb on - strand, within CSW01_08920 at 1927.605 kb on + strand, within CSW01_08920 at 1927.707 kb on - strand at 1927.709 kb on + strand at 1927.709 kb on + strand at 1927.709 kb on + strand at 1927.714 kb on + strand at 1927.714 kb on + strand at 1927.714 kb on + strand at 1927.714 kb on + strand at 1927.745 kb on + strand at 1927.745 kb on + strand at 1927.745 kb on + strand at 1927.745 kb on + strand at 1927.753 kb on - strand at 1927.753 kb on - strand at 1927.753 kb on - strand at 1927.800 kb on + strand at 1927.890 kb on + strand, within CSW01_08925 at 1927.922 kb on + strand, within CSW01_08925 at 1927.930 kb on - strand, within CSW01_08925 at 1927.965 kb on + strand, within CSW01_08925 at 1927.991 kb on + strand, within CSW01_08925 at 1927.994 kb on - strand, within CSW01_08925 at 1928.063 kb on - strand, within CSW01_08925 at 1928.065 kb on + strand, within CSW01_08925 at 1928.073 kb on - strand, within CSW01_08925 at 1928.127 kb on - strand, within CSW01_08925 at 1928.127 kb on - strand, within CSW01_08925 at 1928.132 kb on + strand, within CSW01_08925 at 1928.173 kb on + strand, within CSW01_08925 at 1928.181 kb on - strand, within CSW01_08925 at 1928.209 kb on + strand, within CSW01_08925 at 1928.287 kb on + strand, within CSW01_08925 at 1928.295 kb on - strand, within CSW01_08925 at 1928.401 kb on + strand, within CSW01_08925 at 1928.409 kb on - strand, within CSW01_08925 at 1928.409 kb on - strand, within CSW01_08925 at 1928.409 kb on - strand, within CSW01_08925 at 1928.409 kb on - strand, within CSW01_08925 at 1928.434 kb on + strand, within CSW01_08925 at 1928.442 kb on - strand, within CSW01_08925 at 1928.495 kb on + strand, within CSW01_08925 at 1928.578 kb on - strand, within CSW01_08925 at 1928.599 kb on + strand, within CSW01_08925 at 1928.601 kb on + strand, within CSW01_08925 at 1928.604 kb on + strand, within CSW01_08925 at 1928.607 kb on - strand, within CSW01_08925 at 1928.609 kb on - strand, within CSW01_08925 at 1928.616 kb on + strand, within CSW01_08925 at 1928.664 kb on - strand at 1928.664 kb on - strand at 1929.138 kb on - strand, within CSW01_08935 at 1929.389 kb on - strand, within CSW01_08935 at 1929.580 kb on - strand, within CSW01_08935 at 1929.585 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ICP3_phage_4.1_MOI_rep2 remove 1,926,784 + CSW01_08915 0.69 +1.3 1,926,832 - CSW01_08915 0.73 -0.7 1,926,832 - CSW01_08915 0.73 +1.3 1,926,843 + CSW01_08915 0.74 -0.5 1,926,926 + CSW01_08915 0.81 -1.8 1,927,035 + CSW01_08915 0.89 +0.4 1,927,271 + CSW01_08920 0.17 +0.5 1,927,306 + CSW01_08920 0.24 +0.4 1,927,340 - CSW01_08920 0.30 +1.5 1,927,366 - CSW01_08920 0.35 -3.6 1,927,374 + CSW01_08920 0.37 +1.1 1,927,374 + CSW01_08920 0.37 +0.7 1,927,400 - CSW01_08920 0.42 -0.9 1,927,434 + CSW01_08920 0.48 +0.9 1,927,525 - CSW01_08920 0.66 +1.9 1,927,560 + CSW01_08920 0.72 +1.0 1,927,568 - CSW01_08920 0.74 +0.1 1,927,605 + CSW01_08920 0.81 -0.2 1,927,707 - +0.6 1,927,709 + +0.7 1,927,709 + -0.0 1,927,709 + +0.0 1,927,714 + +1.4 1,927,714 + -0.1 1,927,714 + +1.1 1,927,714 + -2.5 1,927,745 + +0.8 1,927,745 + -2.0 1,927,745 + -0.2 1,927,745 + -0.8 1,927,753 - +0.4 1,927,753 - -1.8 1,927,753 - +2.0 1,927,800 + -3.3 1,927,890 + CSW01_08925 0.14 +1.4 1,927,922 + CSW01_08925 0.17 -0.7 1,927,930 - CSW01_08925 0.18 -1.3 1,927,965 + CSW01_08925 0.22 +1.7 1,927,991 + CSW01_08925 0.24 -0.1 1,927,994 - CSW01_08925 0.25 -0.2 1,928,063 - CSW01_08925 0.32 +1.7 1,928,065 + CSW01_08925 0.32 -0.2 1,928,073 - CSW01_08925 0.33 +1.6 1,928,127 - CSW01_08925 0.39 -0.1 1,928,127 - CSW01_08925 0.39 -1.7 1,928,132 + CSW01_08925 0.39 -1.5 1,928,173 + CSW01_08925 0.43 +0.2 1,928,181 - CSW01_08925 0.44 -2.8 1,928,209 + CSW01_08925 0.47 -2.4 1,928,287 + CSW01_08925 0.55 -0.1 1,928,295 - CSW01_08925 0.56 +0.7 1,928,401 + CSW01_08925 0.67 +1.0 1,928,409 - CSW01_08925 0.68 +1.2 1,928,409 - CSW01_08925 0.68 +1.6 1,928,409 - CSW01_08925 0.68 +0.4 1,928,409 - CSW01_08925 0.68 -2.1 1,928,434 + CSW01_08925 0.70 +1.8 1,928,442 - CSW01_08925 0.71 +0.4 1,928,495 + CSW01_08925 0.77 -0.4 1,928,578 - CSW01_08925 0.85 -1.8 1,928,599 + CSW01_08925 0.87 +1.8 1,928,601 + CSW01_08925 0.88 +0.4 1,928,604 + CSW01_08925 0.88 +1.9 1,928,607 - CSW01_08925 0.88 +0.4 1,928,609 - CSW01_08925 0.88 +0.7 1,928,616 + CSW01_08925 0.89 -1.5 1,928,664 - -0.0 1,928,664 - +1.5 1,929,138 - CSW01_08935 0.28 -2.8 1,929,389 - CSW01_08935 0.63 -0.5 1,929,580 - CSW01_08935 0.89 +0.1 1,929,585 - -3.6
Or see this region's nucleotide sequence