Experiment: ICP3_phage_4.1_MOI_rep2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_08090 and CSW01_08095 are separated by 3 nucleotides CSW01_08095 and CSW01_08100 are separated by 47 nucleotides CSW01_08100 and CSW01_08105 are separated by 208 nucleotides CSW01_08105 and CSW01_08110 are separated by 3 nucleotides
CSW01_08090: CSW01_08090 - ABC transporter permease, at 1,722,759 to 1,723,907
_08090
CSW01_08095: CSW01_08095 - ATP-binding protein, at 1,723,911 to 1,724,360
_08095
CSW01_08100: CSW01_08100 - homoserine O-succinyltransferase, at 1,724,408 to 1,725,367
_08100
CSW01_08105: CSW01_08105 - type IV pilus biogenesis/stability protein PilW, at 1,725,576 to 1,726,289
_08105
CSW01_08110: CSW01_08110 - hypothetical protein, at 1,726,293 to 1,726,517
_08110
Position (kb)
1724
1725
1726 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1723.640 kb on - strand, within CSW01_08090 at 1723.776 kb on - strand, within CSW01_08090 at 1723.776 kb on - strand, within CSW01_08090 at 1723.828 kb on - strand at 1723.840 kb on - strand at 1723.873 kb on + strand at 1723.916 kb on - strand at 1723.923 kb on + strand at 1723.975 kb on - strand, within CSW01_08095 at 1724.065 kb on - strand, within CSW01_08095 at 1724.202 kb on + strand, within CSW01_08095 at 1724.232 kb on + strand, within CSW01_08095 at 1724.275 kb on - strand, within CSW01_08095 at 1724.276 kb on - strand, within CSW01_08095 at 1724.300 kb on + strand, within CSW01_08095 at 1724.416 kb on + strand at 1724.421 kb on + strand at 1724.460 kb on + strand at 1724.558 kb on - strand, within CSW01_08100 at 1724.625 kb on - strand, within CSW01_08100 at 1724.705 kb on - strand, within CSW01_08100 at 1724.757 kb on + strand, within CSW01_08100 at 1724.758 kb on - strand, within CSW01_08100 at 1724.758 kb on - strand, within CSW01_08100 at 1724.811 kb on - strand, within CSW01_08100 at 1724.840 kb on - strand, within CSW01_08100 at 1724.955 kb on + strand, within CSW01_08100 at 1724.958 kb on - strand, within CSW01_08100 at 1724.958 kb on - strand, within CSW01_08100 at 1724.970 kb on + strand, within CSW01_08100 at 1724.983 kb on - strand, within CSW01_08100 at 1725.001 kb on - strand, within CSW01_08100 at 1725.123 kb on - strand, within CSW01_08100 at 1725.296 kb on - strand at 1725.466 kb on - strand at 1725.592 kb on + strand at 1725.644 kb on + strand at 1725.649 kb on + strand, within CSW01_08105 at 1725.657 kb on - strand, within CSW01_08105 at 1725.699 kb on + strand, within CSW01_08105 at 1725.782 kb on - strand, within CSW01_08105 at 1725.791 kb on + strand, within CSW01_08105 at 1725.806 kb on + strand, within CSW01_08105 at 1725.972 kb on + strand, within CSW01_08105 at 1726.176 kb on - strand, within CSW01_08105 at 1726.176 kb on - strand, within CSW01_08105 at 1726.178 kb on + strand, within CSW01_08105 at 1726.291 kb on - strand at 1726.313 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ICP3_phage_4.1_MOI_rep2 remove 1,723,640 - CSW01_08090 0.77 -0.7 1,723,776 - CSW01_08090 0.89 -1.9 1,723,776 - CSW01_08090 0.89 +1.6 1,723,828 - +0.6 1,723,840 - -1.8 1,723,873 + -2.0 1,723,916 - -2.3 1,723,923 + +0.8 1,723,975 - CSW01_08095 0.14 -1.2 1,724,065 - CSW01_08095 0.34 -0.3 1,724,202 + CSW01_08095 0.65 +0.7 1,724,232 + CSW01_08095 0.71 -0.2 1,724,275 - CSW01_08095 0.81 +0.0 1,724,276 - CSW01_08095 0.81 -0.1 1,724,300 + CSW01_08095 0.86 +0.7 1,724,416 + -2.1 1,724,421 + -1.9 1,724,460 + +1.6 1,724,558 - CSW01_08100 0.16 +1.1 1,724,625 - CSW01_08100 0.23 -0.4 1,724,705 - CSW01_08100 0.31 +1.1 1,724,757 + CSW01_08100 0.36 +1.5 1,724,758 - CSW01_08100 0.36 +1.2 1,724,758 - CSW01_08100 0.36 -3.3 1,724,811 - CSW01_08100 0.42 +0.2 1,724,840 - CSW01_08100 0.45 +0.4 1,724,955 + CSW01_08100 0.57 -0.1 1,724,958 - CSW01_08100 0.57 +0.3 1,724,958 - CSW01_08100 0.57 -2.5 1,724,970 + CSW01_08100 0.59 -1.5 1,724,983 - CSW01_08100 0.60 -4.0 1,725,001 - CSW01_08100 0.62 -2.1 1,725,123 - CSW01_08100 0.74 -0.0 1,725,296 - +1.1 1,725,466 - +1.1 1,725,592 + +0.6 1,725,644 + -1.6 1,725,649 + CSW01_08105 0.10 -0.6 1,725,657 - CSW01_08105 0.11 -0.7 1,725,699 + CSW01_08105 0.17 -0.8 1,725,782 - CSW01_08105 0.29 +0.0 1,725,791 + CSW01_08105 0.30 +1.6 1,725,806 + CSW01_08105 0.32 +0.0 1,725,972 + CSW01_08105 0.55 -1.4 1,726,176 - CSW01_08105 0.84 +1.2 1,726,176 - CSW01_08105 0.84 -0.6 1,726,178 + CSW01_08105 0.84 -1.4 1,726,291 - +0.5 1,726,313 - -0.3
Or see this region's nucleotide sequence