Experiment: ICP3_phage_4.1_MOI_rep2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_05305 and CSW01_05310 are separated by 259 nucleotides CSW01_05310 and CSW01_05315 overlap by 38 nucleotides CSW01_05315 and CSW01_05320 are separated by 299 nucleotides
CSW01_05305: CSW01_05305 - long-chain fatty acid transporter, at 1,110,272 to 1,111,558
_05305
CSW01_05310: CSW01_05310 - DUF3379 domain-containing protein, at 1,111,818 to 1,112,579
_05310
CSW01_05315: CSW01_05315 - RNA polymerase subunit sigma, at 1,112,542 to 1,113,108
_05315
CSW01_05320: CSW01_05320 - hypothetical protein, at 1,113,408 to 1,113,588
_05320
Position (kb)
1111
1112
1113 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1110.914 kb on + strand, within CSW01_05305 at 1110.953 kb on - strand, within CSW01_05305 at 1110.958 kb on + strand, within CSW01_05305 at 1110.964 kb on - strand, within CSW01_05305 at 1110.966 kb on - strand, within CSW01_05305 at 1111.025 kb on + strand, within CSW01_05305 at 1111.267 kb on - strand, within CSW01_05305 at 1111.280 kb on + strand, within CSW01_05305 at 1111.288 kb on - strand, within CSW01_05305 at 1111.442 kb on + strand at 1111.471 kb on + strand at 1111.629 kb on + strand at 1111.629 kb on + strand at 1111.637 kb on - strand at 1111.893 kb on + strand at 1111.936 kb on - strand, within CSW01_05310 at 1111.951 kb on + strand, within CSW01_05310 at 1111.964 kb on - strand, within CSW01_05310 at 1111.996 kb on - strand, within CSW01_05310 at 1112.029 kb on + strand, within CSW01_05310 at 1112.226 kb on + strand, within CSW01_05310 at 1112.262 kb on + strand, within CSW01_05310 at 1112.272 kb on - strand, within CSW01_05310 at 1112.473 kb on - strand, within CSW01_05310 at 1112.568 kb on + strand at 1112.568 kb on + strand at 1112.568 kb on + strand at 1112.576 kb on - strand at 1112.576 kb on - strand at 1112.576 kb on - strand at 1112.576 kb on - strand at 1112.576 kb on - strand at 1112.576 kb on - strand at 1112.595 kb on - strand at 1112.769 kb on - strand, within CSW01_05315 at 1112.949 kb on - strand, within CSW01_05315 at 1112.964 kb on + strand, within CSW01_05315 at 1113.011 kb on + strand, within CSW01_05315 at 1113.024 kb on - strand, within CSW01_05315 at 1113.024 kb on - strand, within CSW01_05315 at 1113.033 kb on + strand, within CSW01_05315 at 1113.153 kb on + strand at 1113.214 kb on + strand at 1113.222 kb on - strand at 1113.298 kb on + strand at 1113.300 kb on + strand at 1113.400 kb on + strand at 1113.442 kb on - strand, within CSW01_05320 at 1113.448 kb on + strand, within CSW01_05320 at 1113.448 kb on + strand, within CSW01_05320 at 1113.456 kb on - strand, within CSW01_05320 at 1113.474 kb on - strand, within CSW01_05320
Per-strain Table
Position Strand Gene LocusTag Fraction ICP3_phage_4.1_MOI_rep2 remove 1,110,914 + CSW01_05305 0.50 -1.1 1,110,953 - CSW01_05305 0.53 +2.1 1,110,958 + CSW01_05305 0.53 -0.1 1,110,964 - CSW01_05305 0.54 +0.5 1,110,966 - CSW01_05305 0.54 +0.2 1,111,025 + CSW01_05305 0.59 -1.4 1,111,267 - CSW01_05305 0.77 +0.3 1,111,280 + CSW01_05305 0.78 +0.6 1,111,288 - CSW01_05305 0.79 +3.0 1,111,442 + +0.5 1,111,471 + -0.5 1,111,629 + +0.4 1,111,629 + +0.2 1,111,637 - -0.7 1,111,893 + +0.1 1,111,936 - CSW01_05310 0.15 -1.9 1,111,951 + CSW01_05310 0.17 -0.8 1,111,964 - CSW01_05310 0.19 -0.3 1,111,996 - CSW01_05310 0.23 -1.3 1,112,029 + CSW01_05310 0.28 +0.7 1,112,226 + CSW01_05310 0.54 -3.4 1,112,262 + CSW01_05310 0.58 -1.7 1,112,272 - CSW01_05310 0.60 -1.0 1,112,473 - CSW01_05310 0.86 -1.1 1,112,568 + -2.8 1,112,568 + +0.6 1,112,568 + -1.7 1,112,576 - +0.1 1,112,576 - +0.1 1,112,576 - -3.9 1,112,576 - -0.4 1,112,576 - -2.4 1,112,576 - -0.7 1,112,595 - -1.0 1,112,769 - CSW01_05315 0.40 -0.2 1,112,949 - CSW01_05315 0.72 -2.2 1,112,964 + CSW01_05315 0.74 +1.2 1,113,011 + CSW01_05315 0.83 +0.9 1,113,024 - CSW01_05315 0.85 -2.2 1,113,024 - CSW01_05315 0.85 +1.0 1,113,033 + CSW01_05315 0.87 -0.2 1,113,153 + +0.0 1,113,214 + +1.5 1,113,222 - -1.5 1,113,298 + +1.4 1,113,300 + -2.8 1,113,400 + +1.2 1,113,442 - CSW01_05320 0.19 -0.9 1,113,448 + CSW01_05320 0.22 +1.1 1,113,448 + CSW01_05320 0.22 +0.4 1,113,456 - CSW01_05320 0.27 -4.0 1,113,474 - CSW01_05320 0.36 +0.1
Or see this region's nucleotide sequence