Experiment: ICP3_phage_4.1_MOI_rep2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_02040 and CSW01_02045 are separated by 107 nucleotides CSW01_02045 and CSW01_02050 are separated by 25 nucleotides CSW01_02050 and CSW01_02055 are separated by 473 nucleotides CSW01_02055 and CSW01_02060 are separated by 196 nucleotides
CSW01_02040: CSW01_02040 - 23S ribosomal RNA, at 402,533 to 405,448
_02040
CSW01_02045: CSW01_02045 - 5S ribosomal RNA, at 405,556 to 405,671
_02045
CSW01_02050: CSW01_02050 - tRNA-Asp, at 405,697 to 405,773
_02050
CSW01_02055: CSW01_02055 - sodium:proton antiporter, at 406,247 to 407,581
_02055
CSW01_02060: CSW01_02060 - methionine synthase, at 407,778 to 411,458
_02060
Position (kb) 406
407
408 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 405.974 kb on + strand at 406.013 kb on - strand at 406.147 kb on + strand at 406.277 kb on + strand at 406.389 kb on + strand, within CSW01_02055 at 406.389 kb on + strand, within CSW01_02055 at 406.458 kb on + strand, within CSW01_02055 at 406.458 kb on + strand, within CSW01_02055 at 406.458 kb on + strand, within CSW01_02055 at 406.458 kb on + strand, within CSW01_02055 at 406.458 kb on + strand, within CSW01_02055 at 406.458 kb on + strand, within CSW01_02055 at 406.466 kb on - strand, within CSW01_02055 at 406.493 kb on + strand, within CSW01_02055 at 406.590 kb on - strand, within CSW01_02055 at 406.644 kb on + strand, within CSW01_02055 at 406.744 kb on + strand, within CSW01_02055 at 406.744 kb on + strand, within CSW01_02055 at 406.874 kb on - strand, within CSW01_02055 at 406.945 kb on - strand, within CSW01_02055 at 406.978 kb on + strand, within CSW01_02055 at 406.986 kb on - strand, within CSW01_02055 at 407.001 kb on - strand, within CSW01_02055 at 407.047 kb on + strand, within CSW01_02055 at 407.055 kb on - strand, within CSW01_02055 at 407.227 kb on + strand, within CSW01_02055 at 407.227 kb on + strand, within CSW01_02055 at 407.255 kb on - strand, within CSW01_02055 at 407.349 kb on - strand, within CSW01_02055 at 407.427 kb on - strand, within CSW01_02055 at 407.427 kb on - strand, within CSW01_02055 at 407.508 kb on - strand at 407.508 kb on - strand at 407.573 kb on - strand at 407.573 kb on - strand at 407.573 kb on - strand at 407.886 kb on + strand at 407.968 kb on + strand at 408.028 kb on + strand at 408.036 kb on - strand at 408.057 kb on + strand at 408.069 kb on + strand at 408.082 kb on - strand at 408.087 kb on - strand at 408.231 kb on - strand, within CSW01_02060 at 408.306 kb on + strand, within CSW01_02060 at 408.325 kb on - strand, within CSW01_02060 at 408.345 kb on + strand, within CSW01_02060 at 408.433 kb on + strand, within CSW01_02060 at 408.440 kb on - strand, within CSW01_02060 at 408.480 kb on + strand, within CSW01_02060 at 408.510 kb on + strand, within CSW01_02060 at 408.510 kb on + strand, within CSW01_02060 at 408.518 kb on - strand, within CSW01_02060 at 408.518 kb on - strand, within CSW01_02060
Per-strain Table
Position Strand Gene LocusTag Fraction ICP3_phage_4.1_MOI_rep2 remove 405,974 + -3.2 406,013 - -3.7 406,147 + +0.9 406,277 + +1.9 406,389 + CSW01_02055 0.11 -4.0 406,389 + CSW01_02055 0.11 -0.2 406,458 + CSW01_02055 0.16 -2.0 406,458 + CSW01_02055 0.16 -1.7 406,458 + CSW01_02055 0.16 -0.8 406,458 + CSW01_02055 0.16 +0.8 406,458 + CSW01_02055 0.16 -3.0 406,458 + CSW01_02055 0.16 -0.8 406,466 - CSW01_02055 0.16 +0.2 406,493 + CSW01_02055 0.18 +1.5 406,590 - CSW01_02055 0.26 -1.6 406,644 + CSW01_02055 0.30 +1.3 406,744 + CSW01_02055 0.37 +0.7 406,744 + CSW01_02055 0.37 +1.9 406,874 - CSW01_02055 0.47 -0.2 406,945 - CSW01_02055 0.52 -0.2 406,978 + CSW01_02055 0.55 -3.6 406,986 - CSW01_02055 0.55 +0.7 407,001 - CSW01_02055 0.56 -3.2 407,047 + CSW01_02055 0.60 -2.0 407,055 - CSW01_02055 0.61 +0.3 407,227 + CSW01_02055 0.73 +1.0 407,227 + CSW01_02055 0.73 -3.4 407,255 - CSW01_02055 0.76 -0.3 407,349 - CSW01_02055 0.83 -0.2 407,427 - CSW01_02055 0.88 -4.0 407,427 - CSW01_02055 0.88 -1.7 407,508 - -0.4 407,508 - -0.2 407,573 - +0.8 407,573 - +0.8 407,573 - -1.6 407,886 + +0.4 407,968 + -0.4 408,028 + -0.9 408,036 - +0.3 408,057 + -1.9 408,069 + -4.2 408,082 - +1.0 408,087 - +0.7 408,231 - CSW01_02060 0.12 -1.6 408,306 + CSW01_02060 0.14 +0.2 408,325 - CSW01_02060 0.15 -2.6 408,345 + CSW01_02060 0.15 +0.0 408,433 + CSW01_02060 0.18 -3.5 408,440 - CSW01_02060 0.18 -4.2 408,480 + CSW01_02060 0.19 +1.2 408,510 + CSW01_02060 0.20 -0.2 408,510 + CSW01_02060 0.20 -0.7 408,518 - CSW01_02060 0.20 +1.6 408,518 - CSW01_02060 0.20 +0.6
Or see this region's nucleotide sequence