Experiment: ICP3_phage_4.1_MOI_rep2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_00005 and CSW01_00010 overlap by 31 nucleotides CSW01_00010 and CSW01_00015 are separated by 9 nucleotides CSW01_00015 and CSW01_00020 are separated by 93 nucleotides
CSW01_00005: CSW01_00005 - hypothetical protein, at 235 to 402
_00005
CSW01_00010: CSW01_00010 - FMN-binding protein MioC, at 372 to 806
_00010
CSW01_00015: CSW01_00015 - tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE, at 816 to 2,177
_00015
CSW01_00020: CSW01_00020 - membrane protein insertase YidC, at 2,271 to 3,896
_00020
Position (kb)
0
1
2
3 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 0.353 kb on + strand, within CSW01_00005 at 0.353 kb on + strand, within CSW01_00005 at 0.353 kb on + strand, within CSW01_00005 at 0.361 kb on - strand, within CSW01_00005 at 0.630 kb on + strand, within CSW01_00010 at 0.826 kb on + strand at 0.865 kb on + strand at 0.946 kb on + strand at 1.051 kb on + strand, within CSW01_00015 at 1.088 kb on - strand, within CSW01_00015 at 1.261 kb on - strand, within CSW01_00015 at 1.281 kb on - strand, within CSW01_00015 at 1.281 kb on - strand, within CSW01_00015 at 1.281 kb on - strand, within CSW01_00015 at 1.383 kb on - strand, within CSW01_00015 at 1.426 kb on - strand, within CSW01_00015 at 1.469 kb on - strand, within CSW01_00015 at 1.533 kb on - strand, within CSW01_00015 at 1.535 kb on - strand, within CSW01_00015 at 1.616 kb on - strand, within CSW01_00015 at 1.790 kb on - strand, within CSW01_00015 at 1.876 kb on + strand, within CSW01_00015 at 1.876 kb on + strand, within CSW01_00015 at 1.884 kb on - strand, within CSW01_00015 at 1.934 kb on - strand, within CSW01_00015 at 2.062 kb on - strand at 2.143 kb on + strand at 2.187 kb on - strand at 2.191 kb on - strand at 2.193 kb on + strand at 2.201 kb on - strand at 2.204 kb on + strand at 2.260 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ICP3_phage_4.1_MOI_rep2 remove 353 + CSW01_00005 0.70 -4.0 353 + CSW01_00005 0.70 -0.6 353 + CSW01_00005 0.70 -4.3 361 - CSW01_00005 0.75 -0.4 630 + CSW01_00010 0.59 -1.5 826 + -1.9 865 + -2.0 946 + -1.9 1,051 + CSW01_00015 0.17 -1.7 1,088 - CSW01_00015 0.20 -1.9 1,261 - CSW01_00015 0.33 -1.5 1,281 - CSW01_00015 0.34 -3.0 1,281 - CSW01_00015 0.34 -2.5 1,281 - CSW01_00015 0.34 -1.5 1,383 - CSW01_00015 0.42 -1.7 1,426 - CSW01_00015 0.45 -1.7 1,469 - CSW01_00015 0.48 -2.2 1,533 - CSW01_00015 0.53 -1.3 1,535 - CSW01_00015 0.53 -2.2 1,616 - CSW01_00015 0.59 -2.4 1,790 - CSW01_00015 0.72 -1.9 1,876 + CSW01_00015 0.78 -3.2 1,876 + CSW01_00015 0.78 -0.0 1,884 - CSW01_00015 0.78 -1.9 1,934 - CSW01_00015 0.82 -2.6 2,062 - -1.0 2,143 + -1.6 2,187 - -2.6 2,191 - +1.2 2,193 + -1.6 2,201 - -2.6 2,204 + -3.2 2,260 - +0.1
Or see this region's nucleotide sequence