Experiment: Doxycycline hyclate 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_24800 and AO353_24805 are separated by 141 nucleotides AO353_24805 and AO353_24810 are separated by 92 nucleotides AO353_24810 and AO353_24815 are separated by 5 nucleotides AO353_24815 and AO353_24820 overlap by 4 nucleotides
AO353_24800: AO353_24800 - spermidine/putrescine ABC transporter substrate-binding protein, at 5,467,482 to 5,468,531
_24800
AO353_24805: AO353_24805 - TetR family transcriptional regulator, at 5,468,673 to 5,469,320
_24805
AO353_24810: AO353_24810 - transcriptional regulator, at 5,469,413 to 5,470,129
_24810
AO353_24815: AO353_24815 - amino acid ABC transporter substrate-binding protein, at 5,470,135 to 5,470,908
_24815
AO353_24820: AO353_24820 - histidine kinase, at 5,470,905 to 5,473,166
_24820
Position (kb)
5469
5470
5471 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5468.529 kb on + strand at 5468.529 kb on + strand at 5468.529 kb on + strand at 5468.529 kb on + strand at 5468.530 kb on - strand at 5468.608 kb on - strand at 5468.617 kb on + strand at 5468.836 kb on - strand, within AO353_24805 at 5468.836 kb on - strand, within AO353_24805 at 5468.868 kb on + strand, within AO353_24805 at 5468.868 kb on + strand, within AO353_24805 at 5468.868 kb on + strand, within AO353_24805 at 5468.868 kb on + strand, within AO353_24805 at 5468.868 kb on + strand, within AO353_24805 at 5468.868 kb on + strand, within AO353_24805 at 5468.868 kb on + strand, within AO353_24805 at 5468.869 kb on - strand, within AO353_24805 at 5468.869 kb on - strand, within AO353_24805 at 5468.869 kb on - strand, within AO353_24805 at 5468.869 kb on - strand, within AO353_24805 at 5468.869 kb on - strand, within AO353_24805 at 5468.869 kb on - strand, within AO353_24805 at 5468.869 kb on - strand, within AO353_24805 at 5469.073 kb on - strand, within AO353_24805 at 5469.073 kb on - strand, within AO353_24805 at 5469.091 kb on + strand, within AO353_24805 at 5469.091 kb on + strand, within AO353_24805 at 5469.092 kb on - strand, within AO353_24805 at 5469.103 kb on + strand, within AO353_24805 at 5469.104 kb on - strand, within AO353_24805 at 5469.224 kb on + strand, within AO353_24805 at 5469.341 kb on - strand at 5469.371 kb on + strand at 5469.372 kb on - strand at 5469.372 kb on - strand at 5469.375 kb on - strand at 5469.396 kb on - strand at 5469.396 kb on - strand at 5469.396 kb on - strand at 5469.473 kb on + strand at 5469.473 kb on + strand at 5469.474 kb on - strand at 5469.474 kb on - strand at 5469.477 kb on + strand at 5469.477 kb on + strand at 5469.477 kb on + strand at 5469.478 kb on - strand at 5469.658 kb on + strand, within AO353_24810 at 5469.658 kb on + strand, within AO353_24810 at 5469.707 kb on + strand, within AO353_24810 at 5469.707 kb on + strand, within AO353_24810 at 5469.707 kb on + strand, within AO353_24810 at 5469.707 kb on + strand, within AO353_24810 at 5469.707 kb on + strand, within AO353_24810 at 5469.707 kb on + strand, within AO353_24810 at 5469.708 kb on - strand, within AO353_24810 at 5469.708 kb on - strand, within AO353_24810 at 5469.708 kb on - strand, within AO353_24810 at 5469.708 kb on - strand, within AO353_24810 at 5469.708 kb on - strand, within AO353_24810 at 5469.908 kb on + strand, within AO353_24810 at 5469.924 kb on + strand, within AO353_24810 at 5469.924 kb on + strand, within AO353_24810 at 5469.925 kb on - strand, within AO353_24810 at 5469.925 kb on - strand, within AO353_24810 at 5470.134 kb on + strand at 5470.134 kb on + strand at 5470.134 kb on + strand at 5470.135 kb on - strand at 5470.391 kb on - strand, within AO353_24815 at 5470.516 kb on + strand, within AO353_24815 at 5470.517 kb on - strand, within AO353_24815 at 5470.680 kb on + strand, within AO353_24815 at 5470.681 kb on + strand, within AO353_24815 at 5470.681 kb on + strand, within AO353_24815 at 5470.682 kb on - strand, within AO353_24815 at 5470.682 kb on - strand, within AO353_24815 at 5470.744 kb on + strand, within AO353_24815 at 5470.744 kb on + strand, within AO353_24815 at 5470.745 kb on - strand, within AO353_24815 at 5470.745 kb on - strand, within AO353_24815 at 5471.004 kb on + strand at 5471.004 kb on + strand at 5471.005 kb on - strand at 5471.005 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0002 mg/ml remove 5,468,529 + -1.2 5,468,529 + +1.3 5,468,529 + -0.8 5,468,529 + +0.3 5,468,530 - +1.1 5,468,608 - -0.9 5,468,617 + -0.1 5,468,836 - AO353_24805 0.25 +0.6 5,468,836 - AO353_24805 0.25 -0.3 5,468,868 + AO353_24805 0.30 -0.0 5,468,868 + AO353_24805 0.30 -2.6 5,468,868 + AO353_24805 0.30 -3.1 5,468,868 + AO353_24805 0.30 -1.8 5,468,868 + AO353_24805 0.30 -0.2 5,468,868 + AO353_24805 0.30 +0.1 5,468,868 + AO353_24805 0.30 -0.4 5,468,869 - AO353_24805 0.30 -2.0 5,468,869 - AO353_24805 0.30 -0.8 5,468,869 - AO353_24805 0.30 -0.2 5,468,869 - AO353_24805 0.30 -1.0 5,468,869 - AO353_24805 0.30 +0.6 5,468,869 - AO353_24805 0.30 +1.8 5,468,869 - AO353_24805 0.30 -1.4 5,469,073 - AO353_24805 0.62 -0.4 5,469,073 - AO353_24805 0.62 +1.2 5,469,091 + AO353_24805 0.65 +1.3 5,469,091 + AO353_24805 0.65 +0.8 5,469,092 - AO353_24805 0.65 +1.6 5,469,103 + AO353_24805 0.66 -0.4 5,469,104 - AO353_24805 0.67 +1.6 5,469,224 + AO353_24805 0.85 +1.1 5,469,341 - +0.6 5,469,371 + -0.4 5,469,372 - -0.1 5,469,372 - +0.1 5,469,375 - -0.4 5,469,396 - +0.6 5,469,396 - -1.2 5,469,396 - -0.2 5,469,473 + +0.5 5,469,473 + +0.6 5,469,474 - +1.0 5,469,474 - -0.7 5,469,477 + -0.5 5,469,477 + +0.6 5,469,477 + -2.2 5,469,478 - +1.6 5,469,658 + AO353_24810 0.34 -1.3 5,469,658 + AO353_24810 0.34 -1.4 5,469,707 + AO353_24810 0.41 +1.1 5,469,707 + AO353_24810 0.41 -0.9 5,469,707 + AO353_24810 0.41 -1.2 5,469,707 + AO353_24810 0.41 -1.4 5,469,707 + AO353_24810 0.41 +0.3 5,469,707 + AO353_24810 0.41 -2.4 5,469,708 - AO353_24810 0.41 +0.2 5,469,708 - AO353_24810 0.41 -1.0 5,469,708 - AO353_24810 0.41 -0.8 5,469,708 - AO353_24810 0.41 -0.6 5,469,708 - AO353_24810 0.41 -1.0 5,469,908 + AO353_24810 0.69 -1.0 5,469,924 + AO353_24810 0.71 +1.4 5,469,924 + AO353_24810 0.71 -1.4 5,469,925 - AO353_24810 0.71 -0.2 5,469,925 - AO353_24810 0.71 -0.8 5,470,134 + +2.4 5,470,134 + -0.4 5,470,134 + +0.0 5,470,135 - -1.0 5,470,391 - AO353_24815 0.33 -1.8 5,470,516 + AO353_24815 0.49 -0.5 5,470,517 - AO353_24815 0.49 -1.0 5,470,680 + AO353_24815 0.70 -0.6 5,470,681 + AO353_24815 0.71 -1.4 5,470,681 + AO353_24815 0.71 +0.0 5,470,682 - AO353_24815 0.71 +0.3 5,470,682 - AO353_24815 0.71 -1.8 5,470,744 + AO353_24815 0.79 -0.3 5,470,744 + AO353_24815 0.79 -2.4 5,470,745 - AO353_24815 0.79 -1.6 5,470,745 - AO353_24815 0.79 +2.1 5,471,004 + +0.6 5,471,004 + -1.3 5,471,005 - +0.3 5,471,005 - +0.9
Or see this region's nucleotide sequence