Experiment: Doxycycline hyclate 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_23135 and AO353_23140 are separated by 10 nucleotides AO353_23140 and AO353_23145 are separated by 10 nucleotides AO353_23145 and AO353_23150 overlap by 8 nucleotides AO353_23150 and AO353_23155 are separated by 1 nucleotides
AO353_23135: AO353_23135 - aldehyde dehydrogenase, at 5,076,595 to 5,078,091
_23135
AO353_23140: AO353_23140 - aspartate dehydrogenase, at 5,078,102 to 5,078,905
_23140
AO353_23145: AO353_23145 - hypothetical protein, at 5,078,916 to 5,080,598
_23145
AO353_23150: AO353_23150 - short-chain dehydrogenase, at 5,080,591 to 5,081,388
_23150
AO353_23155: AO353_23155 - IclR family transcriptional regulator, at 5,081,390 to 5,082,232
_23155
Position (kb) 5078
5079
5080
5081 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5078.024 kb on + strand at 5078.072 kb on - strand at 5078.103 kb on + strand at 5078.152 kb on - strand at 5078.152 kb on - strand at 5078.152 kb on - strand at 5078.152 kb on - strand at 5078.476 kb on - strand, within AO353_23140 at 5078.476 kb on - strand, within AO353_23140 at 5078.817 kb on + strand, within AO353_23140 at 5078.818 kb on - strand, within AO353_23140 at 5078.818 kb on - strand, within AO353_23140 at 5078.818 kb on - strand, within AO353_23140 at 5078.818 kb on - strand, within AO353_23140 at 5078.818 kb on - strand, within AO353_23140 at 5078.896 kb on + strand at 5078.896 kb on + strand at 5078.897 kb on - strand at 5078.897 kb on - strand at 5078.897 kb on - strand at 5079.139 kb on + strand, within AO353_23145 at 5079.139 kb on + strand, within AO353_23145 at 5079.139 kb on + strand, within AO353_23145 at 5079.139 kb on + strand, within AO353_23145 at 5079.139 kb on + strand, within AO353_23145 at 5079.139 kb on + strand, within AO353_23145 at 5079.139 kb on + strand, within AO353_23145 at 5079.140 kb on - strand, within AO353_23145 at 5079.140 kb on - strand, within AO353_23145 at 5079.140 kb on - strand, within AO353_23145 at 5079.140 kb on - strand, within AO353_23145 at 5079.157 kb on + strand, within AO353_23145 at 5079.157 kb on + strand, within AO353_23145 at 5079.157 kb on + strand, within AO353_23145 at 5079.673 kb on + strand, within AO353_23145 at 5079.673 kb on + strand, within AO353_23145 at 5079.674 kb on - strand, within AO353_23145 at 5079.717 kb on - strand, within AO353_23145 at 5079.739 kb on + strand, within AO353_23145 at 5079.739 kb on + strand, within AO353_23145 at 5079.739 kb on + strand, within AO353_23145 at 5079.739 kb on + strand, within AO353_23145 at 5079.739 kb on + strand, within AO353_23145 at 5079.740 kb on - strand, within AO353_23145 at 5079.740 kb on - strand, within AO353_23145 at 5079.740 kb on - strand, within AO353_23145 at 5079.740 kb on - strand, within AO353_23145 at 5079.740 kb on - strand, within AO353_23145 at 5080.249 kb on + strand, within AO353_23145 at 5080.249 kb on + strand, within AO353_23145 at 5080.408 kb on + strand, within AO353_23145 at 5080.408 kb on + strand, within AO353_23145 at 5080.408 kb on + strand, within AO353_23145 at 5080.409 kb on - strand, within AO353_23145 at 5080.409 kb on - strand, within AO353_23145 at 5080.583 kb on + strand at 5080.583 kb on + strand at 5080.583 kb on + strand at 5080.584 kb on - strand at 5080.584 kb on - strand at 5080.593 kb on - strand at 5080.593 kb on - strand at 5080.643 kb on + strand at 5080.643 kb on + strand at 5081.379 kb on - strand at 5081.448 kb on + strand at 5081.448 kb on + strand at 5081.449 kb on - strand at 5081.564 kb on - strand, within AO353_23155 at 5081.564 kb on - strand, within AO353_23155
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0002 mg/ml remove 5,078,024 + +0.0 5,078,072 - -0.4 5,078,103 + +0.2 5,078,152 - +0.6 5,078,152 - +0.0 5,078,152 - +0.1 5,078,152 - +0.0 5,078,476 - AO353_23140 0.47 -0.4 5,078,476 - AO353_23140 0.47 +1.0 5,078,817 + AO353_23140 0.89 +0.4 5,078,818 - AO353_23140 0.89 +2.2 5,078,818 - AO353_23140 0.89 +0.6 5,078,818 - AO353_23140 0.89 -1.1 5,078,818 - AO353_23140 0.89 -0.8 5,078,818 - AO353_23140 0.89 -0.4 5,078,896 + -0.7 5,078,896 + +1.4 5,078,897 - -1.4 5,078,897 - +0.6 5,078,897 - +1.0 5,079,139 + AO353_23145 0.13 +1.2 5,079,139 + AO353_23145 0.13 -0.4 5,079,139 + AO353_23145 0.13 -0.8 5,079,139 + AO353_23145 0.13 -0.6 5,079,139 + AO353_23145 0.13 +0.8 5,079,139 + AO353_23145 0.13 -1.4 5,079,139 + AO353_23145 0.13 -2.0 5,079,140 - AO353_23145 0.13 -0.6 5,079,140 - AO353_23145 0.13 -1.0 5,079,140 - AO353_23145 0.13 -0.7 5,079,140 - AO353_23145 0.13 -1.6 5,079,157 + AO353_23145 0.14 +0.1 5,079,157 + AO353_23145 0.14 +0.3 5,079,157 + AO353_23145 0.14 -1.1 5,079,673 + AO353_23145 0.45 -2.1 5,079,673 + AO353_23145 0.45 -2.4 5,079,674 - AO353_23145 0.45 -1.0 5,079,717 - AO353_23145 0.48 -1.7 5,079,739 + AO353_23145 0.49 -1.6 5,079,739 + AO353_23145 0.49 -0.6 5,079,739 + AO353_23145 0.49 +0.6 5,079,739 + AO353_23145 0.49 +0.3 5,079,739 + AO353_23145 0.49 -1.6 5,079,740 - AO353_23145 0.49 +1.8 5,079,740 - AO353_23145 0.49 +0.3 5,079,740 - AO353_23145 0.49 +0.9 5,079,740 - AO353_23145 0.49 -0.1 5,079,740 - AO353_23145 0.49 +0.6 5,080,249 + AO353_23145 0.79 +1.1 5,080,249 + AO353_23145 0.79 -0.7 5,080,408 + AO353_23145 0.89 -1.0 5,080,408 + AO353_23145 0.89 -1.0 5,080,408 + AO353_23145 0.89 +0.3 5,080,409 - AO353_23145 0.89 +0.2 5,080,409 - AO353_23145 0.89 +0.7 5,080,583 + +0.3 5,080,583 + -0.4 5,080,583 + +0.4 5,080,584 - +0.3 5,080,584 - +1.2 5,080,593 - -1.7 5,080,593 - -1.3 5,080,643 + +0.0 5,080,643 + -1.3 5,081,379 - +1.2 5,081,448 + +0.6 5,081,448 + -1.7 5,081,449 - +1.2 5,081,564 - AO353_23155 0.21 +1.6 5,081,564 - AO353_23155 0.21 -1.7
Or see this region's nucleotide sequence