Strain Fitness in Pseudomonas fluorescens FW300-N2E3 around AO353_20625

Experiment: Doxycycline hyclate 0.0002 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAO353_20620 and AO353_20625 are separated by 11 nucleotidesAO353_20625 and AO353_20630 are separated by 141 nucleotidesAO353_20630 and AO353_20635 are separated by 65 nucleotides AO353_20620: AO353_20620 - isopropylmalate isomerase, at 4,523,354 to 4,524,772 _20620 AO353_20625: AO353_20625 - 3-isopropylmalate dehydratase, at 4,524,784 to 4,525,428 _20625 AO353_20630: AO353_20630 - SAM-dependent methyltransferase, at 4,525,570 to 4,526,337 _20630 AO353_20635: AO353_20635 - 3-isopropylmalate dehydrogenase, at 4,526,403 to 4,527,485 _20635 Position (kb) 4524 4525 4526Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4523.915 kb on - strand, within AO353_20620at 4524.092 kb on + strand, within AO353_20620at 4524.092 kb on + strand, within AO353_20620at 4524.092 kb on + strand, within AO353_20620at 4524.092 kb on + strand, within AO353_20620at 4524.092 kb on + strand, within AO353_20620at 4524.093 kb on - strand, within AO353_20620at 4524.093 kb on - strand, within AO353_20620at 4524.093 kb on - strand, within AO353_20620at 4524.341 kb on + strand, within AO353_20620at 4524.341 kb on + strand, within AO353_20620at 4524.341 kb on + strand, within AO353_20620at 4524.341 kb on + strand, within AO353_20620at 4524.341 kb on + strand, within AO353_20620at 4524.342 kb on - strand, within AO353_20620at 4524.342 kb on - strand, within AO353_20620at 4524.342 kb on - strand, within AO353_20620at 4524.342 kb on - strand, within AO353_20620at 4524.342 kb on - strand, within AO353_20620at 4524.662 kb on - strandat 4524.796 kb on - strandat 4525.088 kb on - strand, within AO353_20625at 4525.117 kb on + strand, within AO353_20625at 4525.127 kb on + strand, within AO353_20625at 4525.127 kb on + strand, within AO353_20625at 4525.127 kb on + strand, within AO353_20625at 4525.127 kb on + strand, within AO353_20625at 4525.127 kb on + strand, within AO353_20625at 4525.127 kb on + strand, within AO353_20625at 4525.128 kb on - strand, within AO353_20625at 4525.128 kb on - strand, within AO353_20625at 4525.128 kb on - strand, within AO353_20625at 4525.128 kb on - strand, within AO353_20625at 4525.128 kb on - strand, within AO353_20625at 4525.128 kb on - strand, within AO353_20625at 4525.436 kb on + strandat 4525.437 kb on - strandat 4525.437 kb on - strandat 4525.443 kb on + strandat 4525.501 kb on + strandat 4525.501 kb on + strandat 4525.501 kb on + strandat 4525.502 kb on - strandat 4525.502 kb on - strandat 4525.502 kb on - strandat 4525.502 kb on - strandat 4525.502 kb on - strandat 4525.502 kb on - strandat 4525.598 kb on + strandat 4525.598 kb on + strandat 4525.598 kb on + strandat 4525.598 kb on + strandat 4525.598 kb on + strandat 4525.598 kb on + strandat 4525.599 kb on - strandat 4525.599 kb on - strandat 4525.599 kb on - strandat 4525.601 kb on + strandat 4525.601 kb on + strandat 4525.601 kb on + strandat 4525.602 kb on - strandat 4525.602 kb on - strandat 4525.602 kb on - strandat 4525.602 kb on - strandat 4525.602 kb on - strandat 4525.602 kb on - strandat 4525.602 kb on - strandat 4525.649 kb on + strand, within AO353_20630at 4525.649 kb on + strand, within AO353_20630at 4525.649 kb on + strand, within AO353_20630at 4525.650 kb on - strand, within AO353_20630at 4525.650 kb on - strand, within AO353_20630at 4525.650 kb on - strand, within AO353_20630at 4525.650 kb on - strand, within AO353_20630at 4525.679 kb on + strand, within AO353_20630at 4525.679 kb on + strand, within AO353_20630at 4525.786 kb on + strand, within AO353_20630at 4525.786 kb on + strand, within AO353_20630at 4525.787 kb on - strand, within AO353_20630at 4525.787 kb on - strand, within AO353_20630at 4525.787 kb on - strand, within AO353_20630at 4525.817 kb on + strand, within AO353_20630at 4525.817 kb on + strand, within AO353_20630at 4525.817 kb on + strand, within AO353_20630at 4525.817 kb on + strand, within AO353_20630at 4525.817 kb on + strand, within AO353_20630at 4525.818 kb on - strand, within AO353_20630at 4526.003 kb on + strand, within AO353_20630at 4526.003 kb on + strand, within AO353_20630at 4526.003 kb on + strand, within AO353_20630at 4526.003 kb on + strand, within AO353_20630at 4526.003 kb on + strand, within AO353_20630at 4526.003 kb on + strand, within AO353_20630at 4526.003 kb on + strand, within AO353_20630at 4526.003 kb on + strand, within AO353_20630at 4526.003 kb on + strand, within AO353_20630at 4526.003 kb on + strand, within AO353_20630at 4526.003 kb on + strand, within AO353_20630at 4526.004 kb on - strand, within AO353_20630at 4526.004 kb on - strand, within AO353_20630at 4526.004 kb on - strand, within AO353_20630at 4526.004 kb on - strand, within AO353_20630at 4526.004 kb on - strand, within AO353_20630at 4526.004 kb on - strand, within AO353_20630at 4526.004 kb on - strand, within AO353_20630at 4526.004 kb on - strand, within AO353_20630at 4526.004 kb on - strand, within AO353_20630at 4526.004 kb on - strand, within AO353_20630at 4526.004 kb on - strand, within AO353_20630at 4526.007 kb on + strand, within AO353_20630at 4526.007 kb on + strand, within AO353_20630at 4526.007 kb on + strand, within AO353_20630at 4526.007 kb on + strand, within AO353_20630at 4526.007 kb on + strand, within AO353_20630at 4526.008 kb on - strand, within AO353_20630at 4526.008 kb on - strand, within AO353_20630at 4526.008 kb on - strand, within AO353_20630at 4526.008 kb on - strand, within AO353_20630at 4526.008 kb on - strand, within AO353_20630at 4526.008 kb on - strand, within AO353_20630at 4526.008 kb on - strand, within AO353_20630at 4526.008 kb on - strand, within AO353_20630at 4526.008 kb on - strand, within AO353_20630at 4526.008 kb on - strand, within AO353_20630at 4526.008 kb on - strand, within AO353_20630at 4526.047 kb on + strand, within AO353_20630at 4526.047 kb on + strand, within AO353_20630at 4526.048 kb on - strand, within AO353_20630at 4526.048 kb on - strand, within AO353_20630at 4526.067 kb on - strand, within AO353_20630at 4526.067 kb on - strand, within AO353_20630at 4526.095 kb on + strand, within AO353_20630at 4526.095 kb on + strand, within AO353_20630at 4526.096 kb on - strand, within AO353_20630at 4526.142 kb on + strand, within AO353_20630at 4526.149 kb on - strand, within AO353_20630at 4526.149 kb on - strand, within AO353_20630at 4526.179 kb on + strand, within AO353_20630at 4526.179 kb on + strand, within AO353_20630at 4526.179 kb on + strand, within AO353_20630at 4526.180 kb on - strand, within AO353_20630at 4526.180 kb on - strand, within AO353_20630at 4526.180 kb on - strand, within AO353_20630at 4526.180 kb on - strand, within AO353_20630at 4526.205 kb on + strand, within AO353_20630at 4526.205 kb on + strand, within AO353_20630at 4526.205 kb on + strand, within AO353_20630at 4526.205 kb on + strand, within AO353_20630at 4526.206 kb on - strand, within AO353_20630at 4526.206 kb on - strand, within AO353_20630at 4526.206 kb on - strand, within AO353_20630at 4526.206 kb on - strand, within AO353_20630at 4526.206 kb on - strand, within AO353_20630at 4526.206 kb on - strand, within AO353_20630at 4526.206 kb on - strand, within AO353_20630at 4526.273 kb on - strandat 4526.273 kb on - strandat 4526.273 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0002 mg/ml
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4,523,915 - AO353_20620 0.40 +1.0
4,524,092 + AO353_20620 0.52 -2.5
4,524,092 + AO353_20620 0.52 +0.8
4,524,092 + AO353_20620 0.52 -0.0
4,524,092 + AO353_20620 0.52 +1.4
4,524,092 + AO353_20620 0.52 +1.5
4,524,093 - AO353_20620 0.52 -0.1
4,524,093 - AO353_20620 0.52 -0.6
4,524,093 - AO353_20620 0.52 -1.3
4,524,341 + AO353_20620 0.70 -0.2
4,524,341 + AO353_20620 0.70 +0.6
4,524,341 + AO353_20620 0.70 -0.5
4,524,341 + AO353_20620 0.70 +0.1
4,524,341 + AO353_20620 0.70 +0.2
4,524,342 - AO353_20620 0.70 -2.0
4,524,342 - AO353_20620 0.70 +1.1
4,524,342 - AO353_20620 0.70 -0.2
4,524,342 - AO353_20620 0.70 +0.0
4,524,342 - AO353_20620 0.70 +0.8
4,524,662 - +0.8
4,524,796 - +0.8
4,525,088 - AO353_20625 0.47 -0.2
4,525,117 + AO353_20625 0.52 +0.8
4,525,127 + AO353_20625 0.53 +0.3
4,525,127 + AO353_20625 0.53 +0.4
4,525,127 + AO353_20625 0.53 +0.2
4,525,127 + AO353_20625 0.53 -2.2
4,525,127 + AO353_20625 0.53 -0.2
4,525,127 + AO353_20625 0.53 -2.3
4,525,128 - AO353_20625 0.53 -0.2
4,525,128 - AO353_20625 0.53 -1.8
4,525,128 - AO353_20625 0.53 -2.4
4,525,128 - AO353_20625 0.53 -2.0
4,525,128 - AO353_20625 0.53 -2.4
4,525,128 - AO353_20625 0.53 -2.2
4,525,436 + +1.0
4,525,437 - +0.3
4,525,437 - +0.8
4,525,443 + -0.8
4,525,501 + +1.6
4,525,501 + -0.2
4,525,501 + -0.2
4,525,502 - -0.5
4,525,502 - -0.2
4,525,502 - +0.5
4,525,502 - +0.6
4,525,502 - +0.2
4,525,502 - +1.1
4,525,598 + +0.8
4,525,598 + +0.2
4,525,598 + +0.8
4,525,598 + +0.5
4,525,598 + +0.8
4,525,598 + +0.8
4,525,599 - -1.3
4,525,599 - +0.8
4,525,599 - +0.1
4,525,601 + -0.4
4,525,601 + +0.2
4,525,601 + +1.2
4,525,602 - -0.5
4,525,602 - +0.2
4,525,602 - +1.8
4,525,602 - +0.8
4,525,602 - -0.0
4,525,602 - -0.8
4,525,602 - +2.4
4,525,649 + AO353_20630 0.10 -0.3
4,525,649 + AO353_20630 0.10 -2.1
4,525,649 + AO353_20630 0.10 -0.2
4,525,650 - AO353_20630 0.10 -0.1
4,525,650 - AO353_20630 0.10 -0.4
4,525,650 - AO353_20630 0.10 +0.2
4,525,650 - AO353_20630 0.10 -0.8
4,525,679 + AO353_20630 0.14 -0.2
4,525,679 + AO353_20630 0.14 -0.4
4,525,786 + AO353_20630 0.28 -2.0
4,525,786 + AO353_20630 0.28 +0.3
4,525,787 - AO353_20630 0.28 +1.0
4,525,787 - AO353_20630 0.28 +0.8
4,525,787 - AO353_20630 0.28 -2.0
4,525,817 + AO353_20630 0.32 -0.4
4,525,817 + AO353_20630 0.32 -0.2
4,525,817 + AO353_20630 0.32 +0.4
4,525,817 + AO353_20630 0.32 -0.4
4,525,817 + AO353_20630 0.32 -0.8
4,525,818 - AO353_20630 0.32 +0.9
4,526,003 + AO353_20630 0.56 -0.9
4,526,003 + AO353_20630 0.56 -1.0
4,526,003 + AO353_20630 0.56 -2.4
4,526,003 + AO353_20630 0.56 +0.8
4,526,003 + AO353_20630 0.56 -0.2
4,526,003 + AO353_20630 0.56 +1.0
4,526,003 + AO353_20630 0.56 -1.7
4,526,003 + AO353_20630 0.56 -0.2
4,526,003 + AO353_20630 0.56 +0.3
4,526,003 + AO353_20630 0.56 -0.8
4,526,003 + AO353_20630 0.56 +2.4
4,526,004 - AO353_20630 0.57 -0.5
4,526,004 - AO353_20630 0.57 -1.2
4,526,004 - AO353_20630 0.57 -0.7
4,526,004 - AO353_20630 0.57 +0.8
4,526,004 - AO353_20630 0.57 +0.4
4,526,004 - AO353_20630 0.57 +0.0
4,526,004 - AO353_20630 0.57 +0.1
4,526,004 - AO353_20630 0.57 -0.9
4,526,004 - AO353_20630 0.57 -0.2
4,526,004 - AO353_20630 0.57 -0.8
4,526,004 - AO353_20630 0.57 -1.8
4,526,007 + AO353_20630 0.57 +0.4
4,526,007 + AO353_20630 0.57 +0.2
4,526,007 + AO353_20630 0.57 -0.2
4,526,007 + AO353_20630 0.57 +0.8
4,526,007 + AO353_20630 0.57 -0.8
4,526,008 - AO353_20630 0.57 +0.9
4,526,008 - AO353_20630 0.57 -0.9
4,526,008 - AO353_20630 0.57 -0.2
4,526,008 - AO353_20630 0.57 +0.8
4,526,008 - AO353_20630 0.57 +0.6
4,526,008 - AO353_20630 0.57 -0.2
4,526,008 - AO353_20630 0.57 -0.0
4,526,008 - AO353_20630 0.57 -2.2
4,526,008 - AO353_20630 0.57 +0.9
4,526,008 - AO353_20630 0.57 -0.8
4,526,008 - AO353_20630 0.57 +0.4
4,526,047 + AO353_20630 0.62 +1.2
4,526,047 + AO353_20630 0.62 +2.4
4,526,048 - AO353_20630 0.62 +0.1
4,526,048 - AO353_20630 0.62 -0.3
4,526,067 - AO353_20630 0.65 -2.2
4,526,067 - AO353_20630 0.65 +1.2
4,526,095 + AO353_20630 0.68 -0.8
4,526,095 + AO353_20630 0.68 -1.5
4,526,096 - AO353_20630 0.68 +0.8
4,526,142 + AO353_20630 0.74 -0.2
4,526,149 - AO353_20630 0.75 +2.9
4,526,149 - AO353_20630 0.75 -0.7
4,526,179 + AO353_20630 0.79 +1.0
4,526,179 + AO353_20630 0.79 -0.6
4,526,179 + AO353_20630 0.79 +2.4
4,526,180 - AO353_20630 0.79 -2.5
4,526,180 - AO353_20630 0.79 +0.6
4,526,180 - AO353_20630 0.79 +1.0
4,526,180 - AO353_20630 0.79 -0.4
4,526,205 + AO353_20630 0.83 -0.5
4,526,205 + AO353_20630 0.83 +0.5
4,526,205 + AO353_20630 0.83 +2.1
4,526,205 + AO353_20630 0.83 -1.4
4,526,206 - AO353_20630 0.83 -2.0
4,526,206 - AO353_20630 0.83 +0.3
4,526,206 - AO353_20630 0.83 +0.6
4,526,206 - AO353_20630 0.83 -1.9
4,526,206 - AO353_20630 0.83 -0.8
4,526,206 - AO353_20630 0.83 +0.1
4,526,206 - AO353_20630 0.83 -1.3
4,526,273 - +0.2
4,526,273 - -0.8
4,526,273 - +0.1

Or see this region's nucleotide sequence