Experiment: Doxycycline hyclate 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_17225 and AO353_17230 are separated by 12 nucleotides AO353_17230 and AO353_17235 are separated by 10 nucleotides AO353_17235 and AO353_17240 are separated by 59 nucleotides
AO353_17225: AO353_17225 - 3-carboxy-cis,cis-muconate cycloisomerase, at 3,808,464 to 3,809,825
_17225
AO353_17230: AO353_17230 - Beta-ketoadipate enol-lactone hydrolase (EC 3.1.1.24) (from data) , at 3,809,838 to 3,810,638
_17230
AO353_17235: AO353_17235 - 4-carboxymuconolactone decarboxylase, at 3,810,649 to 3,811,041
_17235
AO353_17240: AO353_17240 - polysaccharide deacetylase, at 3,811,101 to 3,812,000
_17240
Position (kb)
3810
3811
3812 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3809.677 kb on + strand, within AO353_17225 at 3809.677 kb on + strand, within AO353_17225 at 3809.677 kb on + strand, within AO353_17225 at 3809.677 kb on + strand, within AO353_17225 at 3809.677 kb on + strand, within AO353_17225 at 3809.677 kb on + strand, within AO353_17225 at 3809.677 kb on + strand, within AO353_17225 at 3809.678 kb on - strand, within AO353_17225 at 3809.678 kb on - strand, within AO353_17225 at 3809.678 kb on - strand, within AO353_17225 at 3809.678 kb on - strand, within AO353_17225 at 3809.678 kb on - strand, within AO353_17225 at 3809.760 kb on + strand at 3809.760 kb on + strand at 3809.761 kb on - strand at 3809.848 kb on + strand at 3809.848 kb on + strand at 3809.849 kb on - strand at 3809.849 kb on - strand at 3809.849 kb on - strand at 3809.849 kb on - strand at 3809.849 kb on - strand at 3809.849 kb on - strand at 3809.849 kb on - strand at 3809.874 kb on + strand at 3809.874 kb on + strand at 3809.875 kb on - strand at 3809.875 kb on - strand at 3809.875 kb on - strand at 3809.942 kb on + strand, within AO353_17230 at 3809.942 kb on + strand, within AO353_17230 at 3809.942 kb on + strand, within AO353_17230 at 3809.942 kb on + strand, within AO353_17230 at 3809.942 kb on + strand, within AO353_17230 at 3809.942 kb on + strand, within AO353_17230 at 3809.942 kb on + strand, within AO353_17230 at 3809.942 kb on + strand, within AO353_17230 at 3809.942 kb on + strand, within AO353_17230 at 3809.942 kb on + strand, within AO353_17230 at 3809.942 kb on + strand, within AO353_17230 at 3809.942 kb on + strand, within AO353_17230 at 3809.942 kb on + strand, within AO353_17230 at 3809.942 kb on + strand, within AO353_17230 at 3809.942 kb on + strand, within AO353_17230 at 3809.942 kb on + strand, within AO353_17230 at 3809.943 kb on - strand, within AO353_17230 at 3809.943 kb on - strand, within AO353_17230 at 3809.943 kb on - strand, within AO353_17230 at 3809.943 kb on - strand, within AO353_17230 at 3809.943 kb on - strand, within AO353_17230 at 3809.943 kb on - strand, within AO353_17230 at 3809.943 kb on - strand, within AO353_17230 at 3809.943 kb on - strand, within AO353_17230 at 3809.943 kb on - strand, within AO353_17230 at 3809.943 kb on - strand, within AO353_17230 at 3809.943 kb on - strand, within AO353_17230 at 3809.986 kb on + strand, within AO353_17230 at 3810.236 kb on + strand, within AO353_17230 at 3810.236 kb on + strand, within AO353_17230 at 3810.309 kb on - strand, within AO353_17230 at 3810.387 kb on + strand, within AO353_17230 at 3810.387 kb on + strand, within AO353_17230 at 3810.388 kb on - strand, within AO353_17230 at 3810.552 kb on + strand, within AO353_17230 at 3810.552 kb on + strand, within AO353_17230 at 3810.552 kb on + strand, within AO353_17230 at 3810.552 kb on + strand, within AO353_17230 at 3810.632 kb on + strand at 3810.667 kb on + strand at 3810.667 kb on + strand at 3810.667 kb on + strand at 3810.859 kb on - strand, within AO353_17235 at 3810.894 kb on + strand, within AO353_17235 at 3810.894 kb on + strand, within AO353_17235 at 3810.894 kb on + strand, within AO353_17235 at 3810.895 kb on - strand, within AO353_17235 at 3810.958 kb on + strand, within AO353_17235 at 3810.958 kb on + strand, within AO353_17235 at 3810.958 kb on + strand, within AO353_17235 at 3810.959 kb on - strand, within AO353_17235 at 3810.959 kb on - strand, within AO353_17235 at 3811.039 kb on + strand at 3811.217 kb on - strand, within AO353_17240 at 3811.275 kb on + strand, within AO353_17240 at 3811.275 kb on + strand, within AO353_17240 at 3811.275 kb on + strand, within AO353_17240 at 3811.275 kb on + strand, within AO353_17240 at 3811.275 kb on + strand, within AO353_17240 at 3811.279 kb on + strand, within AO353_17240 at 3811.280 kb on - strand, within AO353_17240 at 3811.280 kb on - strand, within AO353_17240 at 3811.280 kb on - strand, within AO353_17240 at 3811.303 kb on + strand, within AO353_17240 at 3811.303 kb on + strand, within AO353_17240 at 3811.386 kb on + strand, within AO353_17240 at 3811.386 kb on + strand, within AO353_17240 at 3811.387 kb on - strand, within AO353_17240 at 3811.446 kb on + strand, within AO353_17240 at 3811.446 kb on + strand, within AO353_17240 at 3811.447 kb on - strand, within AO353_17240 at 3811.447 kb on - strand, within AO353_17240 at 3811.447 kb on - strand, within AO353_17240 at 3811.482 kb on + strand, within AO353_17240 at 3811.482 kb on + strand, within AO353_17240 at 3811.482 kb on + strand, within AO353_17240 at 3811.482 kb on + strand, within AO353_17240 at 3811.482 kb on + strand, within AO353_17240 at 3811.483 kb on - strand, within AO353_17240 at 3811.483 kb on - strand, within AO353_17240 at 3811.483 kb on - strand, within AO353_17240 at 3811.483 kb on - strand, within AO353_17240 at 3811.483 kb on - strand, within AO353_17240 at 3811.483 kb on - strand, within AO353_17240 at 3811.483 kb on - strand, within AO353_17240 at 3811.483 kb on - strand, within AO353_17240 at 3811.483 kb on - strand, within AO353_17240 at 3811.528 kb on + strand, within AO353_17240 at 3811.529 kb on - strand, within AO353_17240 at 3811.529 kb on - strand, within AO353_17240 at 3811.555 kb on - strand, within AO353_17240 at 3811.646 kb on + strand, within AO353_17240 at 3811.646 kb on + strand, within AO353_17240 at 3811.646 kb on + strand, within AO353_17240 at 3811.647 kb on - strand, within AO353_17240 at 3811.756 kb on - strand, within AO353_17240 at 3811.940 kb on + strand at 3811.940 kb on + strand at 3812.016 kb on + strand at 3812.016 kb on + strand at 3812.017 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0002 mg/ml remove 3,809,677 + AO353_17225 0.89 -0.0 3,809,677 + AO353_17225 0.89 -0.1 3,809,677 + AO353_17225 0.89 +0.8 3,809,677 + AO353_17225 0.89 -0.1 3,809,677 + AO353_17225 0.89 -0.9 3,809,677 + AO353_17225 0.89 +1.8 3,809,677 + AO353_17225 0.89 -1.6 3,809,678 - AO353_17225 0.89 +2.2 3,809,678 - AO353_17225 0.89 +1.2 3,809,678 - AO353_17225 0.89 -0.7 3,809,678 - AO353_17225 0.89 -0.6 3,809,678 - AO353_17225 0.89 -0.4 3,809,760 + -0.5 3,809,760 + -0.9 3,809,761 - +0.3 3,809,848 + +0.4 3,809,848 + -0.1 3,809,849 - +0.8 3,809,849 - +0.3 3,809,849 - +0.5 3,809,849 - +0.3 3,809,849 - -0.8 3,809,849 - -0.9 3,809,849 - +0.8 3,809,874 + -0.5 3,809,874 + -0.2 3,809,875 - +1.5 3,809,875 - +1.1 3,809,875 - +0.2 3,809,942 + AO353_17230 0.13 -1.3 3,809,942 + AO353_17230 0.13 +0.9 3,809,942 + AO353_17230 0.13 -0.7 3,809,942 + AO353_17230 0.13 +2.1 3,809,942 + AO353_17230 0.13 +1.3 3,809,942 + AO353_17230 0.13 -0.5 3,809,942 + AO353_17230 0.13 +1.0 3,809,942 + AO353_17230 0.13 +0.1 3,809,942 + AO353_17230 0.13 -0.9 3,809,942 + AO353_17230 0.13 -0.5 3,809,942 + AO353_17230 0.13 +0.2 3,809,942 + AO353_17230 0.13 -0.5 3,809,942 + AO353_17230 0.13 +0.8 3,809,942 + AO353_17230 0.13 +0.2 3,809,942 + AO353_17230 0.13 -1.5 3,809,942 + AO353_17230 0.13 +1.7 3,809,943 - AO353_17230 0.13 +0.3 3,809,943 - AO353_17230 0.13 -1.4 3,809,943 - AO353_17230 0.13 -0.2 3,809,943 - AO353_17230 0.13 +0.2 3,809,943 - AO353_17230 0.13 +1.9 3,809,943 - AO353_17230 0.13 +0.7 3,809,943 - AO353_17230 0.13 -2.7 3,809,943 - AO353_17230 0.13 -0.2 3,809,943 - AO353_17230 0.13 +0.3 3,809,943 - AO353_17230 0.13 +0.2 3,809,943 - AO353_17230 0.13 -0.2 3,809,986 + AO353_17230 0.18 -0.9 3,810,236 + AO353_17230 0.50 +0.5 3,810,236 + AO353_17230 0.50 -1.7 3,810,309 - AO353_17230 0.59 -1.9 3,810,387 + AO353_17230 0.69 +0.3 3,810,387 + AO353_17230 0.69 -0.4 3,810,388 - AO353_17230 0.69 +1.1 3,810,552 + AO353_17230 0.89 -0.1 3,810,552 + AO353_17230 0.89 +0.7 3,810,552 + AO353_17230 0.89 +0.1 3,810,552 + AO353_17230 0.89 +0.5 3,810,632 + +0.7 3,810,667 + +0.8 3,810,667 + +0.1 3,810,667 + +0.1 3,810,859 - AO353_17235 0.53 -0.7 3,810,894 + AO353_17235 0.62 -2.1 3,810,894 + AO353_17235 0.62 -1.5 3,810,894 + AO353_17235 0.62 -2.6 3,810,895 - AO353_17235 0.63 -0.5 3,810,958 + AO353_17235 0.79 +0.2 3,810,958 + AO353_17235 0.79 +0.1 3,810,958 + AO353_17235 0.79 -0.7 3,810,959 - AO353_17235 0.79 -0.8 3,810,959 - AO353_17235 0.79 +0.1 3,811,039 + +0.6 3,811,217 - AO353_17240 0.13 +0.5 3,811,275 + AO353_17240 0.19 +1.6 3,811,275 + AO353_17240 0.19 +1.0 3,811,275 + AO353_17240 0.19 -0.8 3,811,275 + AO353_17240 0.19 -1.3 3,811,275 + AO353_17240 0.19 +0.2 3,811,279 + AO353_17240 0.20 +0.7 3,811,280 - AO353_17240 0.20 -2.3 3,811,280 - AO353_17240 0.20 +2.1 3,811,280 - AO353_17240 0.20 +0.5 3,811,303 + AO353_17240 0.22 +1.2 3,811,303 + AO353_17240 0.22 -1.8 3,811,386 + AO353_17240 0.32 +0.1 3,811,386 + AO353_17240 0.32 -1.3 3,811,387 - AO353_17240 0.32 +0.6 3,811,446 + AO353_17240 0.38 -1.0 3,811,446 + AO353_17240 0.38 -0.7 3,811,447 - AO353_17240 0.38 -2.4 3,811,447 - AO353_17240 0.38 -0.9 3,811,447 - AO353_17240 0.38 -2.4 3,811,482 + AO353_17240 0.42 +1.5 3,811,482 + AO353_17240 0.42 +0.5 3,811,482 + AO353_17240 0.42 +1.5 3,811,482 + AO353_17240 0.42 -0.8 3,811,482 + AO353_17240 0.42 +0.7 3,811,483 - AO353_17240 0.42 -0.2 3,811,483 - AO353_17240 0.42 +0.1 3,811,483 - AO353_17240 0.42 -0.5 3,811,483 - AO353_17240 0.42 -0.5 3,811,483 - AO353_17240 0.42 +1.3 3,811,483 - AO353_17240 0.42 -0.5 3,811,483 - AO353_17240 0.42 +0.1 3,811,483 - AO353_17240 0.42 +0.7 3,811,483 - AO353_17240 0.42 -0.4 3,811,528 + AO353_17240 0.47 -0.1 3,811,529 - AO353_17240 0.48 +1.1 3,811,529 - AO353_17240 0.48 -2.5 3,811,555 - AO353_17240 0.50 +1.2 3,811,646 + AO353_17240 0.61 -0.9 3,811,646 + AO353_17240 0.61 -0.5 3,811,646 + AO353_17240 0.61 +0.5 3,811,647 - AO353_17240 0.61 +0.9 3,811,756 - AO353_17240 0.73 +1.9 3,811,940 + +0.4 3,811,940 + +1.6 3,812,016 + -0.9 3,812,016 + -2.5 3,812,017 - +0.1
Or see this region's nucleotide sequence