Experiment: Cholin acetate 60 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3247 and Shewana3_3248 are separated by 2 nucleotides Shewana3_3248 and Shewana3_3249 are separated by 150 nucleotides Shewana3_3249 and Shewana3_3250 are separated by 19 nucleotides Shewana3_3250 and Shewana3_3251 are separated by 197 nucleotides
Shewana3_3247: Shewana3_3247 - extracellular solute-binding protein (RefSeq), at 3,890,234 to 3,891,091
_3247
Shewana3_3248: Shewana3_3248 - UspA domain-containing protein (RefSeq), at 3,891,094 to 3,891,543
_3248
Shewana3_3249: Shewana3_3249 - UspA domain-containing protein (RefSeq), at 3,891,694 to 3,892,125
_3249
Shewana3_3250: Shewana3_3250 - hypothetical protein (RefSeq), at 3,892,145 to 3,892,543
_3250
Shewana3_3251: Shewana3_3251 - aldehyde dehydrogenase (RefSeq), at 3,892,741 to 3,894,165
_3251
Position (kb)
3891
3892
3893 Strain fitness (log2 ratio)
-1
0
1
2 at 3890.951 kb on - strand, within Shewana3_3247 at 3891.051 kb on - strand at 3891.068 kb on + strand at 3891.358 kb on + strand, within Shewana3_3248 at 3891.365 kb on + strand, within Shewana3_3248 at 3891.677 kb on + strand at 3891.805 kb on + strand, within Shewana3_3249 at 3891.869 kb on - strand, within Shewana3_3249 at 3891.935 kb on + strand, within Shewana3_3249 at 3892.159 kb on + strand at 3892.159 kb on + strand at 3892.182 kb on + strand at 3892.597 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 60 mM remove 3,890,951 - Shewana3_3247 0.84 -1.0 3,891,051 - -0.1 3,891,068 + -0.5 3,891,358 + Shewana3_3248 0.59 -0.2 3,891,365 + Shewana3_3248 0.60 -1.1 3,891,677 + -0.6 3,891,805 + Shewana3_3249 0.26 +0.3 3,891,869 - Shewana3_3249 0.41 -1.2 3,891,935 + Shewana3_3249 0.56 +0.3 3,892,159 + +1.9 3,892,159 + +1.5 3,892,182 + +0.0 3,892,597 - +0.3
Or see this region's nucleotide sequence