Experiment: Cholin acetate 60 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2713 and Shewana3_2714 overlap by 1 nucleotides Shewana3_2714 and Shewana3_2715 are separated by 170 nucleotides
Shewana3_2713: Shewana3_2713 - peptide synthase (RefSeq), at 3,242,653 to 3,244,479
_2713
Shewana3_2714: Shewana3_2714 - alpha/beta hydrolase fold (RefSeq), at 3,244,479 to 3,245,441
_2714
Shewana3_2715: Shewana3_2715 - 3-oxoacyl-(acyl carrier protein) synthase III (RefSeq), at 3,245,612 to 3,246,661
_2715
Position (kb)
3244
3245
3246 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3243.534 kb on - strand, within Shewana3_2713 at 3243.839 kb on + strand, within Shewana3_2713 at 3244.431 kb on - strand at 3244.510 kb on + strand at 3244.576 kb on + strand, within Shewana3_2714 at 3244.584 kb on - strand, within Shewana3_2714 at 3244.978 kb on - strand, within Shewana3_2714 at 3244.983 kb on - strand, within Shewana3_2714 at 3245.028 kb on - strand, within Shewana3_2714 at 3245.091 kb on - strand, within Shewana3_2714 at 3245.132 kb on - strand, within Shewana3_2714 at 3245.174 kb on - strand, within Shewana3_2714 at 3245.206 kb on - strand, within Shewana3_2714 at 3245.367 kb on - strand at 3245.569 kb on - strand at 3245.574 kb on - strand at 3245.592 kb on - strand at 3245.647 kb on - strand at 3245.732 kb on - strand, within Shewana3_2715 at 3245.807 kb on - strand, within Shewana3_2715 at 3245.895 kb on + strand, within Shewana3_2715 at 3246.399 kb on - strand, within Shewana3_2715
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 60 mM remove 3,243,534 - Shewana3_2713 0.48 +0.3 3,243,839 + Shewana3_2713 0.65 +0.3 3,244,431 - -1.6 3,244,510 + +0.3 3,244,576 + Shewana3_2714 0.10 +0.3 3,244,584 - Shewana3_2714 0.11 -0.9 3,244,978 - Shewana3_2714 0.52 +0.7 3,244,983 - Shewana3_2714 0.52 -3.1 3,245,028 - Shewana3_2714 0.57 -0.3 3,245,091 - Shewana3_2714 0.64 +0.3 3,245,132 - Shewana3_2714 0.68 -1.2 3,245,174 - Shewana3_2714 0.72 -3.3 3,245,206 - Shewana3_2714 0.75 +0.3 3,245,367 - +1.6 3,245,569 - -1.2 3,245,574 - +0.3 3,245,592 - -0.7 3,245,647 - -0.8 3,245,732 - Shewana3_2715 0.11 -0.2 3,245,807 - Shewana3_2715 0.19 -0.4 3,245,895 + Shewana3_2715 0.27 -0.7 3,246,399 - Shewana3_2715 0.75 -0.8
Or see this region's nucleotide sequence