Experiment: Cholin acetate 60 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4254 and Shewana3_4255 are separated by 423 nucleotides Shewana3_4255 and Shewana3_4256 are separated by 9 nucleotides Shewana3_4256 and Shewana3_4257 are separated by 0 nucleotides Shewana3_4257 and Shewana3_4258 are separated by 3 nucleotides
Shewana3_4254: Shewana3_4254 - phage integrase family protein (RefSeq), at 131,864 to 133,129
_4254
Shewana3_4255: Shewana3_4255 - hypothetical protein (RefSeq), at 133,553 to 133,855
_4255
Shewana3_4256: Shewana3_4256 - hypothetical protein (RefSeq), at 133,865 to 134,134
_4256
Shewana3_4257: Shewana3_4257 - hypothetical protein (RefSeq), at 134,135 to 134,677
_4257
Shewana3_4258: Shewana3_4258 - TraB pilus assembly family protein (RefSeq), at 134,681 to 136,039
_4258
Position (kb) 133
134
135 Strain fitness (log2 ratio) -1
0
1 at 132.898 kb on + strand, within Shewana3_4254 at 132.909 kb on + strand, within Shewana3_4254 at 132.917 kb on - strand, within Shewana3_4254 at 132.997 kb on - strand, within Shewana3_4254 at 133.282 kb on + strand at 133.655 kb on - strand, within Shewana3_4255 at 133.858 kb on - strand at 133.873 kb on + strand at 133.921 kb on + strand, within Shewana3_4256 at 134.038 kb on - strand, within Shewana3_4256 at 134.275 kb on + strand, within Shewana3_4257 at 134.283 kb on - strand, within Shewana3_4257 at 134.345 kb on + strand, within Shewana3_4257 at 134.371 kb on + strand, within Shewana3_4257 at 134.379 kb on - strand, within Shewana3_4257 at 134.440 kb on - strand, within Shewana3_4257 at 134.540 kb on + strand, within Shewana3_4257 at 134.577 kb on + strand, within Shewana3_4257 at 134.814 kb on + strand at 134.916 kb on - strand, within Shewana3_4258 at 134.923 kb on - strand, within Shewana3_4258 at 135.011 kb on + strand, within Shewana3_4258 at 135.017 kb on + strand, within Shewana3_4258 at 135.111 kb on - strand, within Shewana3_4258
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 60 mM remove 132,898 + Shewana3_4254 0.82 +0.3 132,909 + Shewana3_4254 0.83 -0.5 132,917 - Shewana3_4254 0.83 -0.1 132,997 - Shewana3_4254 0.89 +0.4 133,282 + +0.4 133,655 - Shewana3_4255 0.34 +0.6 133,858 - -0.3 133,873 + -0.2 133,921 + Shewana3_4256 0.21 +0.8 134,038 - Shewana3_4256 0.64 -0.1 134,275 + Shewana3_4257 0.26 -0.9 134,283 - Shewana3_4257 0.27 -0.0 134,345 + Shewana3_4257 0.39 -0.1 134,371 + Shewana3_4257 0.43 +0.2 134,379 - Shewana3_4257 0.45 -0.4 134,440 - Shewana3_4257 0.56 -0.8 134,540 + Shewana3_4257 0.75 -0.8 134,577 + Shewana3_4257 0.81 +0.1 134,814 + +0.1 134,916 - Shewana3_4258 0.17 +0.0 134,923 - Shewana3_4258 0.18 -1.2 135,011 + Shewana3_4258 0.24 -0.5 135,017 + Shewana3_4258 0.25 +0.2 135,111 - Shewana3_4258 0.32 -0.7
Or see this region's nucleotide sequence