Experiment: Cholin acetate 60 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4172 and Shewana3_4173 overlap by 4 nucleotides Shewana3_4173 and Shewana3_4174 are separated by 205 nucleotides Shewana3_4174 and Shewana3_4175 are separated by 240 nucleotides
Shewana3_4172: Shewana3_4172 - DNA-directed DNA polymerase (RefSeq), at 38,365 to 39,615
_4172
Shewana3_4173: Shewana3_4173 - putative prophage repressor (RefSeq), at 39,612 to 40,346
_4173
Shewana3_4174: Shewana3_4174 - hypothetical protein (RefSeq), at 40,552 to 40,911
_4174
Shewana3_4175: Shewana3_4175 - hypothetical protein (RefSeq), at 41,152 to 42,258
_4175
Position (kb) 40
41 Strain fitness (log2 ratio) -2
-1
0
1 at 39.564 kb on + strand at 39.608 kb on - strand at 39.649 kb on - strand at 39.663 kb on - strand at 39.681 kb on + strand at 39.694 kb on + strand, within Shewana3_4173 at 39.747 kb on + strand, within Shewana3_4173 at 39.755 kb on - strand, within Shewana3_4173 at 39.784 kb on + strand, within Shewana3_4173 at 39.852 kb on + strand, within Shewana3_4173 at 40.203 kb on - strand, within Shewana3_4173 at 40.244 kb on + strand, within Shewana3_4173 at 40.304 kb on - strand at 40.459 kb on + strand at 40.467 kb on - strand at 40.477 kb on - strand at 40.526 kb on + strand at 40.544 kb on - strand at 40.544 kb on - strand at 40.544 kb on - strand at 40.544 kb on - strand at 40.573 kb on - strand at 40.791 kb on - strand, within Shewana3_4174 at 40.840 kb on - strand, within Shewana3_4174 at 40.874 kb on - strand, within Shewana3_4174 at 40.881 kb on + strand at 41.088 kb on - strand at 41.271 kb on - strand, within Shewana3_4175 at 41.863 kb on + strand, within Shewana3_4175
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 60 mM remove 39,564 + -0.7 39,608 - -0.6 39,649 - +0.1 39,663 - +0.3 39,681 + -2.4 39,694 + Shewana3_4173 0.11 +0.4 39,747 + Shewana3_4173 0.18 -1.8 39,755 - Shewana3_4173 0.19 -0.2 39,784 + Shewana3_4173 0.23 -0.6 39,852 + Shewana3_4173 0.33 +0.4 40,203 - Shewana3_4173 0.80 +0.0 40,244 + Shewana3_4173 0.86 +0.0 40,304 - -1.2 40,459 + -0.6 40,467 - +0.2 40,477 - +0.8 40,526 + +0.4 40,544 - +0.2 40,544 - +0.1 40,544 - +0.0 40,544 - -0.5 40,573 - +0.0 40,791 - Shewana3_4174 0.66 -1.7 40,840 - Shewana3_4174 0.80 -0.0 40,874 - Shewana3_4174 0.89 -1.6 40,881 + -0.1 41,088 - -1.4 41,271 - Shewana3_4175 0.11 -0.6 41,863 + Shewana3_4175 0.64 -0.1
Or see this region's nucleotide sequence