Experiment: Doxycycline hyclate 0.0015625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt amt1 and Synpcc7942_0443 are separated by 124 nucleotides Synpcc7942_0443 and Synpcc7942_0444 are separated by 39 nucleotides
Synpcc7942_0442: amt1 - ammonium transporter, at 431,458 to 432,951
amt1
Synpcc7942_0443: Synpcc7942_0443 - hypothetical protein, at 433,076 to 434,317
_0443
Synpcc7942_0444: Synpcc7942_0444 - hypothetical protein, at 434,357 to 435,040
_0444
Position (kb) 433
434
435 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 432.100 kb on + strand, within amt1 at 432.140 kb on + strand, within amt1 at 432.203 kb on - strand, within amt1 at 432.217 kb on + strand, within amt1 at 432.222 kb on + strand, within amt1 at 432.225 kb on - strand, within amt1 at 432.225 kb on - strand, within amt1 at 432.230 kb on + strand, within amt1 at 432.242 kb on + strand, within amt1 at 432.266 kb on - strand, within amt1 at 432.297 kb on - strand, within amt1 at 432.297 kb on - strand, within amt1 at 432.297 kb on - strand, within amt1 at 432.359 kb on + strand, within amt1 at 432.378 kb on - strand, within amt1 at 432.404 kb on + strand, within amt1 at 432.404 kb on + strand, within amt1 at 432.412 kb on - strand, within amt1 at 432.412 kb on - strand, within amt1 at 432.412 kb on - strand, within amt1 at 432.414 kb on + strand, within amt1 at 432.435 kb on - strand, within amt1 at 432.457 kb on - strand, within amt1 at 432.469 kb on + strand, within amt1 at 432.477 kb on + strand, within amt1 at 432.516 kb on - strand, within amt1 at 432.538 kb on + strand, within amt1 at 432.609 kb on - strand, within amt1 at 432.696 kb on + strand, within amt1 at 432.717 kb on + strand, within amt1 at 432.717 kb on + strand, within amt1 at 432.717 kb on + strand, within amt1 at 432.725 kb on - strand, within amt1 at 432.725 kb on - strand, within amt1 at 432.727 kb on + strand, within amt1 at 432.738 kb on - strand, within amt1 at 432.777 kb on + strand, within amt1 at 432.795 kb on - strand, within amt1 at 432.824 kb on - strand at 432.864 kb on - strand at 432.865 kb on + strand at 432.873 kb on - strand at 432.907 kb on - strand at 432.928 kb on + strand at 432.952 kb on + strand at 433.054 kb on - strand at 433.057 kb on + strand at 433.103 kb on + strand at 433.181 kb on + strand at 433.181 kb on + strand at 433.186 kb on + strand at 433.189 kb on - strand at 433.189 kb on - strand at 433.189 kb on - strand at 433.189 kb on - strand at 433.194 kb on - strand at 433.204 kb on - strand, within Synpcc7942_0443 at 433.238 kb on - strand, within Synpcc7942_0443 at 433.239 kb on + strand, within Synpcc7942_0443 at 433.245 kb on - strand, within Synpcc7942_0443 at 433.265 kb on + strand at 433.270 kb on - strand, within Synpcc7942_0443 at 433.273 kb on - strand, within Synpcc7942_0443 at 433.281 kb on + strand, within Synpcc7942_0443 at 433.288 kb on - strand, within Synpcc7942_0443 at 433.288 kb on - strand, within Synpcc7942_0443 at 433.305 kb on - strand, within Synpcc7942_0443 at 433.307 kb on + strand, within Synpcc7942_0443 at 433.309 kb on + strand, within Synpcc7942_0443 at 433.315 kb on - strand, within Synpcc7942_0443 at 433.325 kb on - strand, within Synpcc7942_0443 at 433.346 kb on + strand, within Synpcc7942_0443 at 433.373 kb on + strand, within Synpcc7942_0443 at 433.376 kb on - strand, within Synpcc7942_0443 at 433.506 kb on + strand, within Synpcc7942_0443 at 433.512 kb on - strand, within Synpcc7942_0443 at 433.516 kb on + strand, within Synpcc7942_0443 at 433.594 kb on + strand, within Synpcc7942_0443 at 433.594 kb on + strand, within Synpcc7942_0443 at 433.602 kb on - strand, within Synpcc7942_0443 at 433.602 kb on - strand, within Synpcc7942_0443 at 433.603 kb on - strand, within Synpcc7942_0443 at 433.674 kb on + strand, within Synpcc7942_0443 at 433.680 kb on + strand, within Synpcc7942_0443 at 433.750 kb on + strand, within Synpcc7942_0443 at 433.763 kb on - strand, within Synpcc7942_0443 at 433.778 kb on - strand, within Synpcc7942_0443 at 433.788 kb on - strand at 433.811 kb on + strand, within Synpcc7942_0443 at 433.811 kb on + strand, within Synpcc7942_0443 at 433.876 kb on + strand, within Synpcc7942_0443 at 433.882 kb on + strand, within Synpcc7942_0443 at 433.890 kb on - strand, within Synpcc7942_0443 at 433.890 kb on - strand, within Synpcc7942_0443 at 433.918 kb on - strand, within Synpcc7942_0443 at 433.920 kb on - strand, within Synpcc7942_0443 at 433.957 kb on - strand, within Synpcc7942_0443 at 434.009 kb on - strand, within Synpcc7942_0443 at 434.104 kb on + strand, within Synpcc7942_0443 at 434.112 kb on - strand, within Synpcc7942_0443 at 434.153 kb on - strand, within Synpcc7942_0443 at 434.174 kb on + strand, within Synpcc7942_0443 at 434.182 kb on - strand, within Synpcc7942_0443 at 434.182 kb on - strand, within Synpcc7942_0443 at 434.182 kb on - strand, within Synpcc7942_0443 at 434.211 kb on + strand at 434.217 kb on + strand at 434.221 kb on + strand at 434.226 kb on - strand at 434.227 kb on + strand at 434.236 kb on + strand at 434.243 kb on - strand at 434.244 kb on - strand at 434.251 kb on - strand at 434.262 kb on - strand at 434.306 kb on + strand at 434.312 kb on + strand at 434.315 kb on + strand at 434.343 kb on + strand at 434.349 kb on + strand at 434.361 kb on - strand at 434.378 kb on + strand at 434.383 kb on + strand at 434.383 kb on + strand at 434.391 kb on - strand at 434.506 kb on - strand, within Synpcc7942_0444 at 434.516 kb on + strand, within Synpcc7942_0444 at 434.516 kb on + strand, within Synpcc7942_0444 at 434.516 kb on + strand, within Synpcc7942_0444 at 434.522 kb on - strand, within Synpcc7942_0444 at 434.523 kb on + strand, within Synpcc7942_0444 at 434.524 kb on - strand, within Synpcc7942_0444 at 434.524 kb on - strand, within Synpcc7942_0444 at 434.524 kb on - strand, within Synpcc7942_0444 at 434.526 kb on + strand, within Synpcc7942_0444 at 434.526 kb on + strand, within Synpcc7942_0444 at 434.528 kb on + strand, within Synpcc7942_0444 at 434.533 kb on + strand, within Synpcc7942_0444 at 434.534 kb on - strand, within Synpcc7942_0444 at 434.541 kb on - strand, within Synpcc7942_0444 at 434.567 kb on - strand, within Synpcc7942_0444 at 434.652 kb on + strand, within Synpcc7942_0444 at 434.660 kb on - strand, within Synpcc7942_0444 at 434.692 kb on - strand, within Synpcc7942_0444 at 434.731 kb on - strand, within Synpcc7942_0444 at 434.749 kb on + strand, within Synpcc7942_0444 at 434.754 kb on - strand, within Synpcc7942_0444 at 434.789 kb on + strand, within Synpcc7942_0444 at 434.846 kb on - strand, within Synpcc7942_0444 at 434.856 kb on - strand, within Synpcc7942_0444 at 434.875 kb on + strand, within Synpcc7942_0444 at 434.898 kb on + strand, within Synpcc7942_0444 at 434.948 kb on + strand, within Synpcc7942_0444 at 435.016 kb on + strand at 435.016 kb on + strand at 435.016 kb on + strand at 435.018 kb on + strand at 435.026 kb on - strand at 435.043 kb on - strand at 435.117 kb on - strand at 435.308 kb on - strand at 435.310 kb on + strand at 435.310 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0015625 mg/ml remove 432,100 + amt1 Synpcc7942_0442 0.43 +0.2 432,140 + amt1 Synpcc7942_0442 0.46 -0.1 432,203 - amt1 Synpcc7942_0442 0.50 +1.0 432,217 + amt1 Synpcc7942_0442 0.51 +0.1 432,222 + amt1 Synpcc7942_0442 0.51 -2.9 432,225 - amt1 Synpcc7942_0442 0.51 -2.1 432,225 - amt1 Synpcc7942_0442 0.51 -1.1 432,230 + amt1 Synpcc7942_0442 0.52 -0.7 432,242 + amt1 Synpcc7942_0442 0.52 +2.7 432,266 - amt1 Synpcc7942_0442 0.54 +1.2 432,297 - amt1 Synpcc7942_0442 0.56 -0.4 432,297 - amt1 Synpcc7942_0442 0.56 -0.5 432,297 - amt1 Synpcc7942_0442 0.56 -1.3 432,359 + amt1 Synpcc7942_0442 0.60 +0.2 432,378 - amt1 Synpcc7942_0442 0.62 -3.0 432,404 + amt1 Synpcc7942_0442 0.63 +2.2 432,404 + amt1 Synpcc7942_0442 0.63 -1.1 432,412 - amt1 Synpcc7942_0442 0.64 -1.1 432,412 - amt1 Synpcc7942_0442 0.64 +0.9 432,412 - amt1 Synpcc7942_0442 0.64 -0.1 432,414 + amt1 Synpcc7942_0442 0.64 +0.2 432,435 - amt1 Synpcc7942_0442 0.65 +1.0 432,457 - amt1 Synpcc7942_0442 0.67 +0.5 432,469 + amt1 Synpcc7942_0442 0.68 +0.0 432,477 + amt1 Synpcc7942_0442 0.68 -1.1 432,516 - amt1 Synpcc7942_0442 0.71 -1.2 432,538 + amt1 Synpcc7942_0442 0.72 +0.5 432,609 - amt1 Synpcc7942_0442 0.77 -1.9 432,696 + amt1 Synpcc7942_0442 0.83 +1.5 432,717 + amt1 Synpcc7942_0442 0.84 +0.5 432,717 + amt1 Synpcc7942_0442 0.84 -0.7 432,717 + amt1 Synpcc7942_0442 0.84 -1.4 432,725 - amt1 Synpcc7942_0442 0.85 -0.7 432,725 - amt1 Synpcc7942_0442 0.85 +0.3 432,727 + amt1 Synpcc7942_0442 0.85 -0.1 432,738 - amt1 Synpcc7942_0442 0.86 -0.1 432,777 + amt1 Synpcc7942_0442 0.88 +0.6 432,795 - amt1 Synpcc7942_0442 0.89 +0.4 432,824 - -1.6 432,864 - -0.2 432,865 + +0.1 432,873 - -0.8 432,907 - +1.0 432,928 + +0.5 432,952 + +1.1 433,054 - +1.3 433,057 + +2.3 433,103 + +0.1 433,181 + +1.9 433,181 + -0.8 433,186 + -1.8 433,189 - -0.1 433,189 - -0.9 433,189 - -0.7 433,189 - -2.3 433,194 - +0.3 433,204 - Synpcc7942_0443 0.10 +0.6 433,238 - Synpcc7942_0443 0.13 -0.9 433,239 + Synpcc7942_0443 0.13 +0.9 433,245 - Synpcc7942_0443 0.14 -2.1 433,265 + -1.7 433,270 - Synpcc7942_0443 0.16 -0.5 433,273 - Synpcc7942_0443 0.16 -1.7 433,281 + Synpcc7942_0443 0.17 +0.0 433,288 - Synpcc7942_0443 0.17 -1.1 433,288 - Synpcc7942_0443 0.17 +1.6 433,305 - Synpcc7942_0443 0.18 -1.1 433,307 + Synpcc7942_0443 0.19 +2.2 433,309 + Synpcc7942_0443 0.19 -1.9 433,315 - Synpcc7942_0443 0.19 -1.9 433,325 - Synpcc7942_0443 0.20 -0.1 433,346 + Synpcc7942_0443 0.22 -1.7 433,373 + Synpcc7942_0443 0.24 +0.6 433,376 - Synpcc7942_0443 0.24 +1.0 433,506 + Synpcc7942_0443 0.35 +0.3 433,512 - Synpcc7942_0443 0.35 -0.9 433,516 + Synpcc7942_0443 0.35 +0.8 433,594 + Synpcc7942_0443 0.42 -0.3 433,594 + Synpcc7942_0443 0.42 +0.4 433,602 - Synpcc7942_0443 0.42 -0.5 433,602 - Synpcc7942_0443 0.42 +0.9 433,603 - Synpcc7942_0443 0.42 -3.3 433,674 + Synpcc7942_0443 0.48 -2.1 433,680 + Synpcc7942_0443 0.49 -0.1 433,750 + Synpcc7942_0443 0.54 -0.6 433,763 - Synpcc7942_0443 0.55 +0.7 433,778 - Synpcc7942_0443 0.57 +0.4 433,788 - +1.5 433,811 + Synpcc7942_0443 0.59 -0.0 433,811 + Synpcc7942_0443 0.59 +0.6 433,876 + Synpcc7942_0443 0.64 -1.1 433,882 + Synpcc7942_0443 0.65 +0.5 433,890 - Synpcc7942_0443 0.66 +1.1 433,890 - Synpcc7942_0443 0.66 +0.3 433,918 - Synpcc7942_0443 0.68 +0.8 433,920 - Synpcc7942_0443 0.68 +0.5 433,957 - Synpcc7942_0443 0.71 +0.1 434,009 - Synpcc7942_0443 0.75 +0.2 434,104 + Synpcc7942_0443 0.83 +1.6 434,112 - Synpcc7942_0443 0.83 -1.7 434,153 - Synpcc7942_0443 0.87 +0.2 434,174 + Synpcc7942_0443 0.88 +0.2 434,182 - Synpcc7942_0443 0.89 +0.7 434,182 - Synpcc7942_0443 0.89 -1.5 434,182 - Synpcc7942_0443 0.89 -2.4 434,211 + +0.7 434,217 + -1.2 434,221 + -1.1 434,226 - +0.4 434,227 + -0.9 434,236 + -0.1 434,243 - -0.3 434,244 - +1.3 434,251 - +0.1 434,262 - +0.5 434,306 + +0.9 434,312 + +3.0 434,315 + -1.5 434,343 + +1.5 434,349 + -0.8 434,361 - +0.9 434,378 + -0.1 434,383 + -0.3 434,383 + -1.1 434,391 - -0.7 434,506 - Synpcc7942_0444 0.22 -1.1 434,516 + Synpcc7942_0444 0.23 -1.7 434,516 + Synpcc7942_0444 0.23 -1.1 434,516 + Synpcc7942_0444 0.23 -0.9 434,522 - Synpcc7942_0444 0.24 -2.7 434,523 + Synpcc7942_0444 0.24 +0.1 434,524 - Synpcc7942_0444 0.24 -0.8 434,524 - Synpcc7942_0444 0.24 -0.6 434,524 - Synpcc7942_0444 0.24 -2.4 434,526 + Synpcc7942_0444 0.25 -1.9 434,526 + Synpcc7942_0444 0.25 -4.4 434,528 + Synpcc7942_0444 0.25 -3.4 434,533 + Synpcc7942_0444 0.26 -0.4 434,534 - Synpcc7942_0444 0.26 -1.3 434,541 - Synpcc7942_0444 0.27 -0.7 434,567 - Synpcc7942_0444 0.31 -1.7 434,652 + Synpcc7942_0444 0.43 -1.7 434,660 - Synpcc7942_0444 0.44 -3.6 434,692 - Synpcc7942_0444 0.49 -1.7 434,731 - Synpcc7942_0444 0.55 -3.2 434,749 + Synpcc7942_0444 0.57 -2.4 434,754 - Synpcc7942_0444 0.58 -2.4 434,789 + Synpcc7942_0444 0.63 -2.9 434,846 - Synpcc7942_0444 0.71 -1.1 434,856 - Synpcc7942_0444 0.73 -3.1 434,875 + Synpcc7942_0444 0.76 -1.1 434,898 + Synpcc7942_0444 0.79 -1.9 434,948 + Synpcc7942_0444 0.86 +0.9 435,016 + -2.1 435,016 + -3.7 435,016 + -4.4 435,018 + -3.2 435,026 - -2.4 435,043 - -1.0 435,117 - -1.1 435,308 - -0.6 435,310 + -1.2 435,310 + -0.3
Or see this region's nucleotide sequence