Experiment: Doxycycline hyclate 0.0015625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ispE and ksgA overlap by 4 nucleotides ksgA and Synpcc7942_0312 are separated by 26 nucleotides Synpcc7942_0312 and Synpcc7942_0313 are separated by 53 nucleotides
Synpcc7942_0310: ispE - 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, at 309,473 to 310,417
ispE
Synpcc7942_0311: ksgA - dimethyladenosine transferase, at 310,414 to 311,253
ksgA
Synpcc7942_0312: Synpcc7942_0312 - hypothetical protein, at 311,280 to 311,708
_0312
Synpcc7942_0313: Synpcc7942_0313 - hypothetical protein, at 311,762 to 312,274
_0313
Position (kb) 310
311
312 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 309.439 kb on + strand at 309.486 kb on - strand at 309.489 kb on - strand at 310.455 kb on + strand at 310.455 kb on + strand at 310.479 kb on + strand at 310.479 kb on + strand at 310.519 kb on + strand, within ksgA at 310.553 kb on + strand at 310.555 kb on + strand, within ksgA at 310.555 kb on + strand, within ksgA at 310.561 kb on - strand, within ksgA at 310.563 kb on - strand, within ksgA at 310.563 kb on - strand at 310.568 kb on - strand, within ksgA at 310.573 kb on - strand at 310.632 kb on + strand, within ksgA at 310.632 kb on + strand, within ksgA at 310.640 kb on - strand at 310.650 kb on + strand at 310.662 kb on + strand, within ksgA at 310.694 kb on + strand, within ksgA at 310.722 kb on - strand, within ksgA at 310.738 kb on - strand at 310.750 kb on + strand, within ksgA at 310.751 kb on + strand, within ksgA at 310.751 kb on + strand at 310.794 kb on + strand, within ksgA at 310.800 kb on + strand at 310.802 kb on - strand, within ksgA at 310.802 kb on - strand at 310.916 kb on - strand, within ksgA at 310.951 kb on - strand, within ksgA at 311.009 kb on - strand at 311.034 kb on - strand, within ksgA at 311.103 kb on - strand, within ksgA at 311.135 kb on - strand, within ksgA at 311.210 kb on + strand at 311.215 kb on - strand at 311.226 kb on - strand at 311.238 kb on - strand at 311.244 kb on - strand at 311.260 kb on + strand at 311.263 kb on - strand at 311.263 kb on - strand at 311.263 kb on - strand at 311.301 kb on + strand at 311.301 kb on + strand at 311.307 kb on - strand at 311.307 kb on - strand at 311.308 kb on + strand at 311.330 kb on + strand, within Synpcc7942_0312 at 311.330 kb on + strand, within Synpcc7942_0312 at 311.330 kb on - strand, within Synpcc7942_0312 at 311.338 kb on - strand, within Synpcc7942_0312 at 311.338 kb on - strand, within Synpcc7942_0312 at 311.340 kb on + strand, within Synpcc7942_0312 at 311.348 kb on - strand, within Synpcc7942_0312 at 311.348 kb on - strand, within Synpcc7942_0312 at 311.369 kb on + strand at 311.374 kb on + strand, within Synpcc7942_0312 at 311.380 kb on + strand, within Synpcc7942_0312 at 311.384 kb on - strand, within Synpcc7942_0312 at 311.397 kb on + strand, within Synpcc7942_0312 at 311.416 kb on - strand, within Synpcc7942_0312 at 311.429 kb on - strand, within Synpcc7942_0312 at 311.438 kb on + strand, within Synpcc7942_0312 at 311.438 kb on + strand, within Synpcc7942_0312 at 311.471 kb on + strand, within Synpcc7942_0312 at 311.471 kb on + strand, within Synpcc7942_0312 at 311.479 kb on - strand, within Synpcc7942_0312 at 311.492 kb on - strand, within Synpcc7942_0312 at 311.498 kb on - strand, within Synpcc7942_0312 at 311.513 kb on - strand, within Synpcc7942_0312 at 311.519 kb on + strand, within Synpcc7942_0312 at 311.527 kb on - strand, within Synpcc7942_0312 at 311.552 kb on + strand, within Synpcc7942_0312 at 311.571 kb on + strand, within Synpcc7942_0312 at 311.588 kb on + strand, within Synpcc7942_0312 at 311.611 kb on + strand, within Synpcc7942_0312 at 311.621 kb on + strand, within Synpcc7942_0312 at 311.661 kb on + strand, within Synpcc7942_0312 at 311.667 kb on + strand at 311.718 kb on + strand at 311.719 kb on - strand at 311.726 kb on + strand at 311.733 kb on + strand at 311.741 kb on - strand at 311.763 kb on - strand at 311.795 kb on - strand at 311.811 kb on + strand at 311.839 kb on + strand, within Synpcc7942_0313 at 311.839 kb on + strand, within Synpcc7942_0313 at 311.842 kb on - strand, within Synpcc7942_0313 at 311.850 kb on + strand, within Synpcc7942_0313 at 311.924 kb on - strand, within Synpcc7942_0313 at 311.974 kb on - strand, within Synpcc7942_0313 at 311.981 kb on + strand, within Synpcc7942_0313 at 311.985 kb on + strand, within Synpcc7942_0313 at 312.019 kb on - strand, within Synpcc7942_0313 at 312.036 kb on + strand, within Synpcc7942_0313 at 312.036 kb on + strand, within Synpcc7942_0313 at 312.044 kb on - strand, within Synpcc7942_0313 at 312.049 kb on - strand, within Synpcc7942_0313 at 312.054 kb on - strand, within Synpcc7942_0313 at 312.060 kb on - strand, within Synpcc7942_0313 at 312.076 kb on + strand, within Synpcc7942_0313 at 312.081 kb on + strand, within Synpcc7942_0313 at 312.084 kb on - strand, within Synpcc7942_0313 at 312.133 kb on + strand, within Synpcc7942_0313 at 312.133 kb on + strand, within Synpcc7942_0313 at 312.133 kb on + strand, within Synpcc7942_0313 at 312.133 kb on + strand, within Synpcc7942_0313 at 312.141 kb on - strand, within Synpcc7942_0313 at 312.141 kb on - strand, within Synpcc7942_0313 at 312.141 kb on - strand, within Synpcc7942_0313 at 312.141 kb on - strand, within Synpcc7942_0313 at 312.141 kb on - strand, within Synpcc7942_0313 at 312.141 kb on - strand, within Synpcc7942_0313 at 312.156 kb on - strand, within Synpcc7942_0313 at 312.173 kb on + strand, within Synpcc7942_0313
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0015625 mg/ml remove 309,439 + -0.6 309,486 - +2.0 309,489 - -2.4 310,455 + +1.0 310,455 + +0.4 310,479 + -1.7 310,479 + -2.4 310,519 + ksgA Synpcc7942_0311 0.12 -2.4 310,553 + -2.1 310,555 + ksgA Synpcc7942_0311 0.17 -1.1 310,555 + ksgA Synpcc7942_0311 0.17 -2.0 310,561 - ksgA Synpcc7942_0311 0.17 -1.1 310,563 - ksgA Synpcc7942_0311 0.18 -0.1 310,563 - -1.7 310,568 - ksgA Synpcc7942_0311 0.18 -0.1 310,573 - -2.1 310,632 + ksgA Synpcc7942_0311 0.26 -1.6 310,632 + ksgA Synpcc7942_0311 0.26 -0.1 310,640 - -1.1 310,650 + -1.7 310,662 + ksgA Synpcc7942_0311 0.30 -3.3 310,694 + ksgA Synpcc7942_0311 0.33 -0.7 310,722 - ksgA Synpcc7942_0311 0.37 -2.4 310,738 - -1.1 310,750 + ksgA Synpcc7942_0311 0.40 -2.7 310,751 + ksgA Synpcc7942_0311 0.40 -3.7 310,751 + -0.1 310,794 + ksgA Synpcc7942_0311 0.45 -1.3 310,800 + -1.1 310,802 - ksgA Synpcc7942_0311 0.46 -1.0 310,802 - -2.1 310,916 - ksgA Synpcc7942_0311 0.60 -2.1 310,951 - ksgA Synpcc7942_0311 0.64 -2.7 311,009 - -0.1 311,034 - ksgA Synpcc7942_0311 0.74 -2.7 311,103 - ksgA Synpcc7942_0311 0.82 -3.3 311,135 - ksgA Synpcc7942_0311 0.86 -2.1 311,210 + -2.4 311,215 - -2.4 311,226 - -2.4 311,238 - +0.1 311,244 - +0.4 311,260 + +1.3 311,263 - -0.1 311,263 - +0.6 311,263 - +1.3 311,301 + +0.3 311,301 + -1.0 311,307 - +0.7 311,307 - +0.7 311,308 + +1.1 311,330 + Synpcc7942_0312 0.12 -0.4 311,330 + Synpcc7942_0312 0.12 -1.7 311,330 - Synpcc7942_0312 0.12 -2.4 311,338 - Synpcc7942_0312 0.14 -1.1 311,338 - Synpcc7942_0312 0.14 -1.1 311,340 + Synpcc7942_0312 0.14 +0.2 311,348 - Synpcc7942_0312 0.16 +0.3 311,348 - Synpcc7942_0312 0.16 -0.9 311,369 + -0.1 311,374 + Synpcc7942_0312 0.22 -0.1 311,380 + Synpcc7942_0312 0.23 +0.1 311,384 - Synpcc7942_0312 0.24 +1.2 311,397 + Synpcc7942_0312 0.27 -0.1 311,416 - Synpcc7942_0312 0.32 -2.4 311,429 - Synpcc7942_0312 0.35 -0.4 311,438 + Synpcc7942_0312 0.37 -1.4 311,438 + Synpcc7942_0312 0.37 +1.3 311,471 + Synpcc7942_0312 0.45 +1.1 311,471 + Synpcc7942_0312 0.45 -0.0 311,479 - Synpcc7942_0312 0.46 -0.1 311,492 - Synpcc7942_0312 0.49 -0.1 311,498 - Synpcc7942_0312 0.51 +0.5 311,513 - Synpcc7942_0312 0.54 +0.3 311,519 + Synpcc7942_0312 0.56 -0.1 311,527 - Synpcc7942_0312 0.58 -0.9 311,552 + Synpcc7942_0312 0.63 +0.3 311,571 + Synpcc7942_0312 0.68 +0.2 311,588 + Synpcc7942_0312 0.72 +0.4 311,611 + Synpcc7942_0312 0.77 -3.3 311,621 + Synpcc7942_0312 0.79 -1.6 311,661 + Synpcc7942_0312 0.89 +0.9 311,667 + -0.9 311,718 + -1.3 311,719 - +0.5 311,726 + +0.3 311,733 + -0.9 311,741 - +1.0 311,763 - +0.7 311,795 - +1.1 311,811 + +1.1 311,839 + Synpcc7942_0313 0.15 +1.2 311,839 + Synpcc7942_0313 0.15 +0.5 311,842 - Synpcc7942_0313 0.16 -0.3 311,850 + Synpcc7942_0313 0.17 +1.7 311,924 - Synpcc7942_0313 0.32 -2.4 311,974 - Synpcc7942_0313 0.41 -0.0 311,981 + Synpcc7942_0313 0.43 -0.1 311,985 + Synpcc7942_0313 0.43 +0.0 312,019 - Synpcc7942_0313 0.50 -0.4 312,036 + Synpcc7942_0313 0.53 +1.1 312,036 + Synpcc7942_0313 0.53 -0.3 312,044 - Synpcc7942_0313 0.55 +0.2 312,049 - Synpcc7942_0313 0.56 -1.7 312,054 - Synpcc7942_0313 0.57 +0.4 312,060 - Synpcc7942_0313 0.58 +0.5 312,076 + Synpcc7942_0313 0.61 +0.9 312,081 + Synpcc7942_0313 0.62 -2.3 312,084 - Synpcc7942_0313 0.63 +0.8 312,133 + Synpcc7942_0313 0.72 +0.9 312,133 + Synpcc7942_0313 0.72 -2.1 312,133 + Synpcc7942_0313 0.72 -1.5 312,133 + Synpcc7942_0313 0.72 +1.2 312,141 - Synpcc7942_0313 0.74 +0.3 312,141 - Synpcc7942_0313 0.74 +2.7 312,141 - Synpcc7942_0313 0.74 -0.7 312,141 - Synpcc7942_0313 0.74 -0.6 312,141 - Synpcc7942_0313 0.74 -1.7 312,141 - Synpcc7942_0313 0.74 +0.3 312,156 - Synpcc7942_0313 0.77 -1.1 312,173 + Synpcc7942_0313 0.80 +1.1
Or see this region's nucleotide sequence