Experiment: D-Mannose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_6041 and Pf1N1B4_6042 are separated by 194 nucleotides Pf1N1B4_6042 and Pf1N1B4_6043 are separated by 113 nucleotides Pf1N1B4_6043 and Pf1N1B4_6044 are separated by 230 nucleotides Pf1N1B4_6044 and Pf1N1B4_6045 are separated by 243 nucleotides
Pf1N1B4_6041 - Carbon storage regulator, at 504,393 to 504,587
_6041
Pf1N1B4_6042 - Probable transmembrane protein, at 504,782 to 505,237
_6042
Pf1N1B4_6043 - membrane protein, putative, at 505,351 to 506,565
_6043
Pf1N1B4_6044 - hypothetical protein, at 506,796 to 507,065
_6044
Pf1N1B4_6045 - CigR (Putative inner membrane protein), at 507,309 to 507,545
_6045
Position (kb) 505
506
507 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 504.369 kb on + strand at 504.370 kb on - strand at 504.400 kb on + strand at 504.427 kb on + strand, within Pf1N1B4_6041 at 504.428 kb on - strand, within Pf1N1B4_6041 at 504.446 kb on + strand, within Pf1N1B4_6041 at 504.447 kb on - strand, within Pf1N1B4_6041 at 504.711 kb on - strand at 504.854 kb on - strand, within Pf1N1B4_6042 at 504.872 kb on + strand at 504.873 kb on - strand, within Pf1N1B4_6042 at 504.873 kb on - strand, within Pf1N1B4_6042 at 504.873 kb on - strand, within Pf1N1B4_6042 at 505.015 kb on - strand, within Pf1N1B4_6042 at 505.015 kb on - strand, within Pf1N1B4_6042 at 505.018 kb on - strand, within Pf1N1B4_6042 at 505.018 kb on - strand, within Pf1N1B4_6042 at 505.018 kb on - strand, within Pf1N1B4_6042 at 505.152 kb on - strand, within Pf1N1B4_6042 at 505.152 kb on - strand, within Pf1N1B4_6042 at 505.244 kb on - strand at 505.244 kb on - strand at 505.244 kb on - strand at 505.244 kb on - strand at 505.244 kb on - strand at 505.244 kb on - strand at 505.251 kb on - strand at 505.273 kb on + strand at 505.273 kb on + strand at 505.274 kb on - strand at 505.274 kb on - strand at 505.274 kb on - strand at 505.274 kb on - strand at 505.278 kb on + strand at 505.281 kb on + strand at 505.281 kb on + strand at 505.282 kb on - strand at 505.282 kb on - strand at 505.282 kb on - strand at 505.282 kb on - strand at 505.282 kb on - strand at 505.282 kb on - strand at 505.297 kb on - strand at 505.297 kb on - strand at 505.297 kb on - strand at 505.297 kb on - strand at 505.363 kb on + strand at 505.364 kb on - strand at 505.364 kb on - strand at 505.378 kb on - strand at 505.378 kb on - strand at 505.378 kb on - strand at 505.378 kb on - strand at 505.580 kb on - strand, within Pf1N1B4_6043 at 505.616 kb on - strand, within Pf1N1B4_6043 at 505.637 kb on - strand, within Pf1N1B4_6043 at 505.637 kb on - strand, within Pf1N1B4_6043 at 505.714 kb on + strand at 505.729 kb on + strand, within Pf1N1B4_6043 at 505.730 kb on - strand, within Pf1N1B4_6043 at 505.730 kb on - strand, within Pf1N1B4_6043 at 505.735 kb on + strand, within Pf1N1B4_6043 at 505.736 kb on - strand, within Pf1N1B4_6043 at 505.736 kb on - strand at 505.753 kb on + strand, within Pf1N1B4_6043 at 506.083 kb on + strand, within Pf1N1B4_6043 at 506.084 kb on - strand, within Pf1N1B4_6043 at 506.084 kb on - strand, within Pf1N1B4_6043 at 506.104 kb on + strand, within Pf1N1B4_6043 at 506.104 kb on + strand, within Pf1N1B4_6043 at 506.104 kb on + strand, within Pf1N1B4_6043 at 506.105 kb on - strand, within Pf1N1B4_6043 at 506.105 kb on - strand, within Pf1N1B4_6043 at 506.186 kb on + strand at 506.206 kb on - strand, within Pf1N1B4_6043 at 506.206 kb on - strand, within Pf1N1B4_6043 at 506.206 kb on - strand, within Pf1N1B4_6043 at 506.206 kb on - strand, within Pf1N1B4_6043 at 506.309 kb on + strand, within Pf1N1B4_6043 at 506.309 kb on + strand, within Pf1N1B4_6043 at 506.309 kb on + strand, within Pf1N1B4_6043 at 506.310 kb on - strand at 506.310 kb on - strand, within Pf1N1B4_6043 at 506.310 kb on - strand, within Pf1N1B4_6043 at 506.311 kb on + strand, within Pf1N1B4_6043 at 506.311 kb on + strand, within Pf1N1B4_6043 at 506.311 kb on + strand, within Pf1N1B4_6043 at 506.311 kb on + strand, within Pf1N1B4_6043 at 506.311 kb on + strand, within Pf1N1B4_6043 at 506.311 kb on + strand, within Pf1N1B4_6043 at 506.311 kb on + strand, within Pf1N1B4_6043 at 506.311 kb on + strand, within Pf1N1B4_6043 at 506.312 kb on - strand, within Pf1N1B4_6043 at 506.312 kb on - strand, within Pf1N1B4_6043 at 506.312 kb on - strand at 506.312 kb on - strand, within Pf1N1B4_6043 at 506.312 kb on - strand, within Pf1N1B4_6043 at 506.312 kb on - strand, within Pf1N1B4_6043 at 506.315 kb on + strand, within Pf1N1B4_6043 at 506.315 kb on + strand, within Pf1N1B4_6043 at 506.315 kb on + strand, within Pf1N1B4_6043 at 506.315 kb on + strand, within Pf1N1B4_6043 at 506.315 kb on + strand, within Pf1N1B4_6043 at 506.315 kb on + strand, within Pf1N1B4_6043 at 506.315 kb on + strand, within Pf1N1B4_6043 at 506.316 kb on - strand, within Pf1N1B4_6043 at 506.316 kb on - strand, within Pf1N1B4_6043 at 506.316 kb on - strand, within Pf1N1B4_6043 at 506.316 kb on - strand, within Pf1N1B4_6043 at 506.316 kb on - strand, within Pf1N1B4_6043 at 506.563 kb on + strand at 506.563 kb on + strand at 506.563 kb on + strand at 506.563 kb on + strand at 506.563 kb on + strand at 506.564 kb on - strand at 506.666 kb on + strand at 506.667 kb on - strand at 507.153 kb on + strand at 507.165 kb on + strand at 507.165 kb on + strand at 507.165 kb on + strand at 507.166 kb on - strand at 507.166 kb on - strand at 507.261 kb on + strand at 507.261 kb on + strand at 507.261 kb on + strand at 507.262 kb on - strand at 507.262 kb on - strand at 507.272 kb on - strand at 507.272 kb on - strand at 507.337 kb on + strand, within Pf1N1B4_6045 at 507.338 kb on - strand, within Pf1N1B4_6045 at 507.406 kb on + strand, within Pf1N1B4_6045 at 507.407 kb on - strand at 507.407 kb on - strand, within Pf1N1B4_6045 at 507.407 kb on - strand, within Pf1N1B4_6045
Per-strain Table
Position Strand Gene LocusTag Fraction D-Mannose (C) remove 504,369 + +0.3 504,370 - +1.3 504,400 + +0.5 504,427 + Pf1N1B4_6041 0.17 -1.8 504,428 - Pf1N1B4_6041 0.18 -0.6 504,446 + Pf1N1B4_6041 0.27 -0.2 504,447 - Pf1N1B4_6041 0.28 +0.8 504,711 - -1.0 504,854 - Pf1N1B4_6042 0.16 +2.1 504,872 + -0.2 504,873 - Pf1N1B4_6042 0.20 -2.4 504,873 - Pf1N1B4_6042 0.20 -1.5 504,873 - Pf1N1B4_6042 0.20 -2.2 505,015 - Pf1N1B4_6042 0.51 +0.8 505,015 - Pf1N1B4_6042 0.51 -0.1 505,018 - Pf1N1B4_6042 0.52 -0.6 505,018 - Pf1N1B4_6042 0.52 +0.4 505,018 - Pf1N1B4_6042 0.52 +0.8 505,152 - Pf1N1B4_6042 0.81 -0.2 505,152 - Pf1N1B4_6042 0.81 +1.1 505,244 - -0.2 505,244 - +1.8 505,244 - +0.3 505,244 - -1.0 505,244 - -0.6 505,244 - -0.2 505,251 - +1.3 505,273 + -1.2 505,273 + -0.3 505,274 - -1.8 505,274 - -1.2 505,274 - -0.5 505,274 - +0.3 505,278 + -0.7 505,281 + +1.8 505,281 + -0.2 505,282 - -0.2 505,282 - +2.6 505,282 - +2.1 505,282 - +0.4 505,282 - +0.3 505,282 - -0.2 505,297 - -0.6 505,297 - -1.2 505,297 - +0.0 505,297 - -1.2 505,363 + -1.2 505,364 - +1.3 505,364 - -0.2 505,378 - -1.2 505,378 - -0.8 505,378 - -0.2 505,378 - +0.0 505,580 - Pf1N1B4_6043 0.19 +0.8 505,616 - Pf1N1B4_6043 0.22 -0.8 505,637 - Pf1N1B4_6043 0.24 +2.2 505,637 - Pf1N1B4_6043 0.24 -0.7 505,714 + -0.2 505,729 + Pf1N1B4_6043 0.31 -0.2 505,730 - Pf1N1B4_6043 0.31 -0.2 505,730 - Pf1N1B4_6043 0.31 -0.2 505,735 + Pf1N1B4_6043 0.32 -3.0 505,736 - Pf1N1B4_6043 0.32 -1.0 505,736 - -0.2 505,753 + Pf1N1B4_6043 0.33 +0.8 506,083 + Pf1N1B4_6043 0.60 -0.2 506,084 - Pf1N1B4_6043 0.60 -0.2 506,084 - Pf1N1B4_6043 0.60 -1.1 506,104 + Pf1N1B4_6043 0.62 -0.7 506,104 + Pf1N1B4_6043 0.62 -0.5 506,104 + Pf1N1B4_6043 0.62 -0.0 506,105 - Pf1N1B4_6043 0.62 +0.8 506,105 - Pf1N1B4_6043 0.62 -0.2 506,186 + +0.8 506,206 - Pf1N1B4_6043 0.70 +0.5 506,206 - Pf1N1B4_6043 0.70 +1.8 506,206 - Pf1N1B4_6043 0.70 -1.8 506,206 - Pf1N1B4_6043 0.70 -1.2 506,309 + Pf1N1B4_6043 0.79 -0.7 506,309 + Pf1N1B4_6043 0.79 +0.7 506,309 + Pf1N1B4_6043 0.79 +0.1 506,310 - +0.8 506,310 - Pf1N1B4_6043 0.79 -1.8 506,310 - Pf1N1B4_6043 0.79 +0.3 506,311 + Pf1N1B4_6043 0.79 -0.2 506,311 + Pf1N1B4_6043 0.79 -0.2 506,311 + Pf1N1B4_6043 0.79 -1.2 506,311 + Pf1N1B4_6043 0.79 +0.5 506,311 + Pf1N1B4_6043 0.79 -1.2 506,311 + Pf1N1B4_6043 0.79 +0.3 506,311 + Pf1N1B4_6043 0.79 -0.2 506,311 + Pf1N1B4_6043 0.79 -1.2 506,312 - Pf1N1B4_6043 0.79 -1.8 506,312 - Pf1N1B4_6043 0.79 +0.3 506,312 - +1.3 506,312 - Pf1N1B4_6043 0.79 -1.2 506,312 - Pf1N1B4_6043 0.79 +0.8 506,312 - Pf1N1B4_6043 0.79 +0.3 506,315 + Pf1N1B4_6043 0.79 -0.8 506,315 + Pf1N1B4_6043 0.79 +0.3 506,315 + Pf1N1B4_6043 0.79 -0.9 506,315 + Pf1N1B4_6043 0.79 +1.3 506,315 + Pf1N1B4_6043 0.79 -0.2 506,315 + Pf1N1B4_6043 0.79 +1.8 506,315 + Pf1N1B4_6043 0.79 -0.5 506,316 - Pf1N1B4_6043 0.79 -0.4 506,316 - Pf1N1B4_6043 0.79 +1.8 506,316 - Pf1N1B4_6043 0.79 -0.2 506,316 - Pf1N1B4_6043 0.79 -1.6 506,316 - Pf1N1B4_6043 0.79 -0.7 506,563 + +0.3 506,563 + -0.2 506,563 + +0.8 506,563 + -1.0 506,563 + -1.2 506,564 - -0.7 506,666 + -1.2 506,667 - +1.8 507,153 + -1.6 507,165 + +0.9 507,165 + +0.8 507,165 + -2.6 507,166 - +0.9 507,166 - -1.8 507,261 + +0.3 507,261 + -0.2 507,261 + -0.6 507,262 - -2.8 507,262 - -0.2 507,272 - +1.8 507,272 - +0.8 507,337 + Pf1N1B4_6045 0.12 -0.2 507,338 - Pf1N1B4_6045 0.12 +0.2 507,406 + Pf1N1B4_6045 0.41 +0.0 507,407 - -1.2 507,407 - Pf1N1B4_6045 0.41 -0.1 507,407 - Pf1N1B4_6045 0.41 -0.2
Or see this region's nucleotide sequence