Experiment: D-Mannose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5132 and Pf1N1B4_5133 are separated by 131 nucleotides Pf1N1B4_5133 and Pf1N1B4_5134 are separated by 13 nucleotides Pf1N1B4_5134 and Pf1N1B4_5135 overlap by 8 nucleotides
Pf1N1B4_5132 - Transcriptional regulator, AraC family, at 5,710,848 to 5,711,864
_5132
Pf1N1B4_5133 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 5,711,996 to 5,713,180
_5133
Pf1N1B4_5134 - 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157), at 5,713,194 to 5,714,045
_5134
Pf1N1B4_5135 - Transcriptional regulator, TetR family, at 5,714,038 to 5,714,685
_5135
Position (kb)
5711
5712
5713
5714 Strain fitness (log2 ratio)
-2
-1
0
1 at 5711.686 kb on - strand at 5711.742 kb on - strand, within Pf1N1B4_5132 at 5711.827 kb on + strand at 5711.932 kb on - strand at 5711.954 kb on + strand at 5711.980 kb on + strand at 5711.980 kb on + strand at 5711.980 kb on + strand at 5711.981 kb on - strand at 5712.194 kb on + strand, within Pf1N1B4_5133 at 5712.194 kb on + strand, within Pf1N1B4_5133 at 5712.194 kb on + strand, within Pf1N1B4_5133 at 5712.195 kb on - strand, within Pf1N1B4_5133 at 5712.371 kb on + strand, within Pf1N1B4_5133 at 5712.428 kb on + strand, within Pf1N1B4_5133 at 5712.476 kb on - strand at 5712.635 kb on - strand, within Pf1N1B4_5133 at 5713.718 kb on + strand, within Pf1N1B4_5134 at 5713.936 kb on - strand, within Pf1N1B4_5134 at 5714.038 kb on - strand at 5714.168 kb on - strand, within Pf1N1B4_5135
Per-strain Table
Position Strand Gene LocusTag Fraction D-Mannose (C) remove 5,711,686 - +1.5 5,711,742 - Pf1N1B4_5132 0.88 +0.4 5,711,827 + -2.1 5,711,932 - +0.7 5,711,954 + -1.7 5,711,980 + -1.9 5,711,980 + -0.0 5,711,980 + +0.5 5,711,981 - +1.5 5,712,194 + Pf1N1B4_5133 0.17 -1.5 5,712,194 + Pf1N1B4_5133 0.17 -1.8 5,712,194 + Pf1N1B4_5133 0.17 -0.3 5,712,195 - Pf1N1B4_5133 0.17 -1.3 5,712,371 + Pf1N1B4_5133 0.32 -2.1 5,712,428 + Pf1N1B4_5133 0.36 -1.5 5,712,476 - -0.5 5,712,635 - Pf1N1B4_5133 0.54 +0.8 5,713,718 + Pf1N1B4_5134 0.62 +0.2 5,713,936 - Pf1N1B4_5134 0.87 -0.9 5,714,038 - +1.1 5,714,168 - Pf1N1B4_5135 0.20 +1.5
Or see this region's nucleotide sequence