Experiment: D-Mannose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5038 and Pf1N1B4_5039 are separated by 190 nucleotides Pf1N1B4_5039 and Pf1N1B4_5040 are separated by 86 nucleotides Pf1N1B4_5040 and Pf1N1B4_5041 are separated by 31 nucleotides Pf1N1B4_5041 and Pf1N1B4_5042 overlap by 1 nucleotides
Pf1N1B4_5038 - Various polyols ABC transporter, periplasmic substrate-binding protein, at 5,582,893 to 5,584,203
_5038
Pf1N1B4_5039 - Multiple polyol-specific dehydrogenase (EC 1.1.1.-), at 5,584,394 to 5,585,167
_5039
Pf1N1B4_5040 - Inosine-5'-monophosphate dehydrogenase (EC 1.1.1.205), at 5,585,254 to 5,585,694
_5040
Pf1N1B4_5041 - Nitrite reductase [NAD(P)H] small subunit (EC 1.7.1.4), at 5,585,726 to 5,586,112
_5041
Pf1N1B4_5042 - Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4), at 5,586,112 to 5,588,811
_5042
Position (kb)
5584
5585
5586 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5583.787 kb on + strand, within Pf1N1B4_5038 at 5583.787 kb on + strand, within Pf1N1B4_5038 at 5583.788 kb on - strand, within Pf1N1B4_5038 at 5583.872 kb on - strand, within Pf1N1B4_5038 at 5583.872 kb on - strand, within Pf1N1B4_5038 at 5583.937 kb on + strand, within Pf1N1B4_5038 at 5583.937 kb on + strand at 5583.938 kb on - strand at 5583.949 kb on + strand, within Pf1N1B4_5038 at 5583.949 kb on + strand, within Pf1N1B4_5038 at 5583.950 kb on - strand, within Pf1N1B4_5038 at 5583.950 kb on - strand, within Pf1N1B4_5038 at 5583.950 kb on - strand, within Pf1N1B4_5038 at 5584.036 kb on + strand, within Pf1N1B4_5038 at 5584.037 kb on - strand, within Pf1N1B4_5038 at 5584.052 kb on - strand at 5584.202 kb on - strand at 5584.368 kb on - strand at 5584.370 kb on - strand at 5584.376 kb on + strand at 5584.470 kb on - strand at 5584.470 kb on - strand at 5584.470 kb on - strand at 5584.557 kb on - strand at 5584.703 kb on + strand, within Pf1N1B4_5039 at 5584.744 kb on + strand, within Pf1N1B4_5039 at 5584.745 kb on - strand, within Pf1N1B4_5039 at 5584.851 kb on - strand, within Pf1N1B4_5039 at 5584.994 kb on + strand, within Pf1N1B4_5039 at 5585.043 kb on + strand, within Pf1N1B4_5039 at 5585.117 kb on + strand at 5585.117 kb on + strand at 5585.117 kb on + strand at 5585.135 kb on + strand at 5585.135 kb on + strand at 5585.135 kb on + strand at 5585.136 kb on - strand at 5585.136 kb on - strand at 5585.270 kb on + strand at 5585.270 kb on + strand at 5585.271 kb on - strand at 5585.271 kb on - strand at 5585.422 kb on - strand, within Pf1N1B4_5040 at 5585.519 kb on + strand, within Pf1N1B4_5040 at 5585.520 kb on - strand, within Pf1N1B4_5040 at 5585.579 kb on - strand, within Pf1N1B4_5040 at 5585.579 kb on - strand, within Pf1N1B4_5040 at 5585.579 kb on - strand, within Pf1N1B4_5040 at 5585.579 kb on - strand, within Pf1N1B4_5040 at 5585.579 kb on - strand, within Pf1N1B4_5040 at 5585.838 kb on + strand, within Pf1N1B4_5041 at 5585.839 kb on - strand, within Pf1N1B4_5041 at 5585.839 kb on - strand, within Pf1N1B4_5041 at 5585.839 kb on - strand, within Pf1N1B4_5041 at 5585.839 kb on - strand, within Pf1N1B4_5041 at 5585.937 kb on + strand, within Pf1N1B4_5041 at 5585.938 kb on - strand, within Pf1N1B4_5041
Per-strain Table
Position Strand Gene LocusTag Fraction D-Mannose (C) remove 5,583,787 + Pf1N1B4_5038 0.68 +0.2 5,583,787 + Pf1N1B4_5038 0.68 +1.3 5,583,788 - Pf1N1B4_5038 0.68 +2.0 5,583,872 - Pf1N1B4_5038 0.75 +1.8 5,583,872 - Pf1N1B4_5038 0.75 -1.5 5,583,937 + Pf1N1B4_5038 0.80 -1.1 5,583,937 + -0.5 5,583,938 - +0.9 5,583,949 + Pf1N1B4_5038 0.81 -0.5 5,583,949 + Pf1N1B4_5038 0.81 +0.2 5,583,950 - Pf1N1B4_5038 0.81 -0.9 5,583,950 - Pf1N1B4_5038 0.81 +0.8 5,583,950 - Pf1N1B4_5038 0.81 +0.4 5,584,036 + Pf1N1B4_5038 0.87 +0.5 5,584,037 - Pf1N1B4_5038 0.87 -0.1 5,584,052 - -0.5 5,584,202 - +1.3 5,584,368 - +0.5 5,584,370 - +0.1 5,584,376 + -1.2 5,584,470 - -2.1 5,584,470 - +0.1 5,584,470 - -0.5 5,584,557 - +0.5 5,584,703 + Pf1N1B4_5039 0.40 -1.8 5,584,744 + Pf1N1B4_5039 0.45 +0.2 5,584,745 - Pf1N1B4_5039 0.45 -0.5 5,584,851 - Pf1N1B4_5039 0.59 -1.1 5,584,994 + Pf1N1B4_5039 0.78 -0.1 5,585,043 + Pf1N1B4_5039 0.84 +0.2 5,585,117 + -0.5 5,585,117 + +2.0 5,585,117 + -0.9 5,585,135 + +0.4 5,585,135 + +0.9 5,585,135 + +0.5 5,585,136 - +0.5 5,585,136 - +0.5 5,585,270 + +1.8 5,585,270 + -0.5 5,585,271 - -0.5 5,585,271 - +0.1 5,585,422 - Pf1N1B4_5040 0.38 -2.7 5,585,519 + Pf1N1B4_5040 0.60 +1.1 5,585,520 - Pf1N1B4_5040 0.60 -0.2 5,585,579 - Pf1N1B4_5040 0.74 -0.5 5,585,579 - Pf1N1B4_5040 0.74 -0.5 5,585,579 - Pf1N1B4_5040 0.74 +0.8 5,585,579 - Pf1N1B4_5040 0.74 -1.1 5,585,579 - Pf1N1B4_5040 0.74 -0.9 5,585,838 + Pf1N1B4_5041 0.29 +0.2 5,585,839 - Pf1N1B4_5041 0.29 -0.5 5,585,839 - Pf1N1B4_5041 0.29 +0.1 5,585,839 - Pf1N1B4_5041 0.29 +0.8 5,585,839 - Pf1N1B4_5041 0.29 +0.2 5,585,937 + Pf1N1B4_5041 0.55 +0.3 5,585,938 - Pf1N1B4_5041 0.55 +1.1
Or see this region's nucleotide sequence