Experiment: D-Mannose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1473 and Pf1N1B4_1474 are separated by 316 nucleotides Pf1N1B4_1474 and Pf1N1B4_1475 are separated by 216 nucleotides Pf1N1B4_1475 and Pf1N1B4_1476 overlap by 6 nucleotides
Pf1N1B4_1473 - ADP-heptose--lipooligosaccharide heptosyltransferase II (EC 2.4.1.-), at 1,687,770 to 1,688,804
_1473
Pf1N1B4_1474 - Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42), at 1,689,121 to 1,692,060
_1474
Pf1N1B4_1475 - FIG00962376: hypothetical protein, at 1,692,277 to 1,692,450
_1475
Pf1N1B4_1476 - Pyruvate dehydrogenase E1 component (EC 1.2.4.1), at 1,692,445 to 1,695,090
_1476
Position (kb)
1689
1690
1691
1692
1693 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1688.835 kb on - strand at 1688.922 kb on - strand at 1688.922 kb on - strand at 1688.922 kb on - strand at 1688.922 kb on - strand at 1688.925 kb on - strand at 1689.194 kb on - strand at 1689.248 kb on + strand at 1689.249 kb on - strand at 1689.249 kb on - strand at 1689.310 kb on + strand at 1689.310 kb on + strand at 1689.311 kb on - strand at 1689.311 kb on - strand at 1689.378 kb on - strand at 1689.387 kb on + strand at 1689.405 kb on + strand at 1689.749 kb on + strand, within Pf1N1B4_1474 at 1689.901 kb on - strand, within Pf1N1B4_1474 at 1689.901 kb on - strand, within Pf1N1B4_1474 at 1689.993 kb on - strand, within Pf1N1B4_1474 at 1690.377 kb on - strand, within Pf1N1B4_1474 at 1690.739 kb on + strand, within Pf1N1B4_1474 at 1690.785 kb on + strand, within Pf1N1B4_1474 at 1690.786 kb on - strand, within Pf1N1B4_1474 at 1690.957 kb on + strand, within Pf1N1B4_1474 at 1690.958 kb on - strand, within Pf1N1B4_1474 at 1690.960 kb on + strand, within Pf1N1B4_1474 at 1690.961 kb on - strand, within Pf1N1B4_1474 at 1691.268 kb on - strand, within Pf1N1B4_1474 at 1691.268 kb on - strand, within Pf1N1B4_1474 at 1691.271 kb on - strand, within Pf1N1B4_1474 at 1691.271 kb on - strand, within Pf1N1B4_1474 at 1691.271 kb on - strand, within Pf1N1B4_1474 at 1691.603 kb on + strand, within Pf1N1B4_1474 at 1691.603 kb on + strand, within Pf1N1B4_1474 at 1691.604 kb on - strand, within Pf1N1B4_1474 at 1691.604 kb on - strand, within Pf1N1B4_1474 at 1691.604 kb on - strand, within Pf1N1B4_1474 at 1691.604 kb on - strand, within Pf1N1B4_1474 at 1692.026 kb on - strand at 1692.026 kb on - strand at 1692.066 kb on - strand at 1692.096 kb on + strand at 1692.096 kb on + strand at 1692.097 kb on - strand at 1692.097 kb on - strand at 1692.097 kb on - strand at 1692.228 kb on + strand at 1692.252 kb on + strand at 1692.275 kb on + strand at 1692.288 kb on + strand at 1692.347 kb on + strand, within Pf1N1B4_1475 at 1692.347 kb on + strand, within Pf1N1B4_1475 at 1692.347 kb on + strand, within Pf1N1B4_1475 at 1692.347 kb on + strand, within Pf1N1B4_1475 at 1692.347 kb on + strand, within Pf1N1B4_1475 at 1692.347 kb on + strand, within Pf1N1B4_1475 at 1692.347 kb on + strand, within Pf1N1B4_1475 at 1692.347 kb on + strand, within Pf1N1B4_1475 at 1692.347 kb on + strand, within Pf1N1B4_1475 at 1692.347 kb on + strand, within Pf1N1B4_1475 at 1692.347 kb on + strand, within Pf1N1B4_1475 at 1692.347 kb on + strand, within Pf1N1B4_1475 at 1692.347 kb on + strand, within Pf1N1B4_1475 at 1692.347 kb on + strand, within Pf1N1B4_1475 at 1692.348 kb on - strand, within Pf1N1B4_1475 at 1692.370 kb on + strand, within Pf1N1B4_1475 at 1692.370 kb on + strand, within Pf1N1B4_1475 at 1692.370 kb on + strand, within Pf1N1B4_1475 at 1692.370 kb on + strand, within Pf1N1B4_1475 at 1692.370 kb on + strand, within Pf1N1B4_1475 at 1692.796 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Mannose (C) remove 1,688,835 - -1.3 1,688,922 - +1.6 1,688,922 - -0.7 1,688,922 - +0.3 1,688,922 - +0.6 1,688,925 - -2.3 1,689,194 - -0.7 1,689,248 + +1.6 1,689,249 - +0.3 1,689,249 - +1.3 1,689,310 + +1.6 1,689,310 + -1.1 1,689,311 - -0.5 1,689,311 - +0.8 1,689,378 - -0.7 1,689,387 + +0.3 1,689,405 + -2.7 1,689,749 + Pf1N1B4_1474 0.21 +0.3 1,689,901 - Pf1N1B4_1474 0.27 -2.1 1,689,901 - Pf1N1B4_1474 0.27 -0.7 1,689,993 - Pf1N1B4_1474 0.30 -1.3 1,690,377 - Pf1N1B4_1474 0.43 +0.3 1,690,739 + Pf1N1B4_1474 0.55 +0.3 1,690,785 + Pf1N1B4_1474 0.57 +0.3 1,690,786 - Pf1N1B4_1474 0.57 -1.3 1,690,957 + Pf1N1B4_1474 0.62 +0.3 1,690,958 - Pf1N1B4_1474 0.62 -2.3 1,690,960 + Pf1N1B4_1474 0.63 -0.9 1,690,961 - Pf1N1B4_1474 0.63 +0.3 1,691,268 - Pf1N1B4_1474 0.73 -2.1 1,691,268 - Pf1N1B4_1474 0.73 -0.7 1,691,271 - Pf1N1B4_1474 0.73 -1.5 1,691,271 - Pf1N1B4_1474 0.73 -1.1 1,691,271 - Pf1N1B4_1474 0.73 -0.7 1,691,603 + Pf1N1B4_1474 0.84 -1.7 1,691,603 + Pf1N1B4_1474 0.84 -0.7 1,691,604 - Pf1N1B4_1474 0.84 -0.1 1,691,604 - Pf1N1B4_1474 0.84 -1.3 1,691,604 - Pf1N1B4_1474 0.84 -1.3 1,691,604 - Pf1N1B4_1474 0.84 +0.3 1,692,026 - +1.0 1,692,026 - -0.1 1,692,066 - +0.6 1,692,096 + -0.3 1,692,096 + +1.1 1,692,097 - -1.0 1,692,097 - +0.5 1,692,097 - +2.3 1,692,228 + +0.3 1,692,252 + -2.3 1,692,275 + -1.1 1,692,288 + +1.6 1,692,347 + Pf1N1B4_1475 0.40 -1.3 1,692,347 + Pf1N1B4_1475 0.40 -0.7 1,692,347 + Pf1N1B4_1475 0.40 -0.5 1,692,347 + Pf1N1B4_1475 0.40 -0.5 1,692,347 + Pf1N1B4_1475 0.40 -0.5 1,692,347 + Pf1N1B4_1475 0.40 -1.3 1,692,347 + Pf1N1B4_1475 0.40 -0.3 1,692,347 + Pf1N1B4_1475 0.40 -1.3 1,692,347 + Pf1N1B4_1475 0.40 -1.1 1,692,347 + Pf1N1B4_1475 0.40 +1.1 1,692,347 + Pf1N1B4_1475 0.40 +0.7 1,692,347 + Pf1N1B4_1475 0.40 -1.1 1,692,347 + Pf1N1B4_1475 0.40 +0.4 1,692,347 + Pf1N1B4_1475 0.40 +0.3 1,692,348 - Pf1N1B4_1475 0.41 +0.7 1,692,370 + Pf1N1B4_1475 0.53 -0.7 1,692,370 + Pf1N1B4_1475 0.53 -0.7 1,692,370 + Pf1N1B4_1475 0.53 +0.9 1,692,370 + Pf1N1B4_1475 0.53 -0.1 1,692,370 + Pf1N1B4_1475 0.53 +0.6 1,692,796 + +0.3
Or see this region's nucleotide sequence