Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1474

Experiment: D-Mannose (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1473 and Pf1N1B4_1474 are separated by 316 nucleotidesPf1N1B4_1474 and Pf1N1B4_1475 are separated by 216 nucleotidesPf1N1B4_1475 and Pf1N1B4_1476 overlap by 6 nucleotides Pf1N1B4_1473 - ADP-heptose--lipooligosaccharide heptosyltransferase II (EC 2.4.1.-), at 1,687,770 to 1,688,804 _1473 Pf1N1B4_1474 - Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42), at 1,689,121 to 1,692,060 _1474 Pf1N1B4_1475 - FIG00962376: hypothetical protein, at 1,692,277 to 1,692,450 _1475 Pf1N1B4_1476 - Pyruvate dehydrogenase E1 component (EC 1.2.4.1), at 1,692,445 to 1,695,090 _1476 Position (kb) 1689 1690 1691 1692 1693Strain fitness (log2 ratio) -2 -1 0 1 2at 1688.835 kb on - strandat 1688.922 kb on - strandat 1688.922 kb on - strandat 1688.922 kb on - strandat 1688.922 kb on - strandat 1688.925 kb on - strandat 1689.194 kb on - strandat 1689.248 kb on + strandat 1689.249 kb on - strandat 1689.249 kb on - strandat 1689.310 kb on + strandat 1689.310 kb on + strandat 1689.311 kb on - strandat 1689.311 kb on - strandat 1689.378 kb on - strandat 1689.387 kb on + strandat 1689.405 kb on + strandat 1689.749 kb on + strand, within Pf1N1B4_1474at 1689.901 kb on - strand, within Pf1N1B4_1474at 1689.901 kb on - strand, within Pf1N1B4_1474at 1689.993 kb on - strand, within Pf1N1B4_1474at 1690.377 kb on - strand, within Pf1N1B4_1474at 1690.739 kb on + strand, within Pf1N1B4_1474at 1690.785 kb on + strand, within Pf1N1B4_1474at 1690.786 kb on - strand, within Pf1N1B4_1474at 1690.957 kb on + strand, within Pf1N1B4_1474at 1690.958 kb on - strand, within Pf1N1B4_1474at 1690.960 kb on + strand, within Pf1N1B4_1474at 1690.961 kb on - strand, within Pf1N1B4_1474at 1691.268 kb on - strand, within Pf1N1B4_1474at 1691.268 kb on - strand, within Pf1N1B4_1474at 1691.271 kb on - strand, within Pf1N1B4_1474at 1691.271 kb on - strand, within Pf1N1B4_1474at 1691.271 kb on - strand, within Pf1N1B4_1474at 1691.603 kb on + strand, within Pf1N1B4_1474at 1691.603 kb on + strand, within Pf1N1B4_1474at 1691.604 kb on - strand, within Pf1N1B4_1474at 1691.604 kb on - strand, within Pf1N1B4_1474at 1691.604 kb on - strand, within Pf1N1B4_1474at 1691.604 kb on - strand, within Pf1N1B4_1474at 1692.026 kb on - strandat 1692.026 kb on - strandat 1692.066 kb on - strandat 1692.096 kb on + strandat 1692.096 kb on + strandat 1692.097 kb on - strandat 1692.097 kb on - strandat 1692.097 kb on - strandat 1692.228 kb on + strandat 1692.252 kb on + strandat 1692.275 kb on + strandat 1692.288 kb on + strandat 1692.347 kb on + strand, within Pf1N1B4_1475at 1692.347 kb on + strand, within Pf1N1B4_1475at 1692.347 kb on + strand, within Pf1N1B4_1475at 1692.347 kb on + strand, within Pf1N1B4_1475at 1692.347 kb on + strand, within Pf1N1B4_1475at 1692.347 kb on + strand, within Pf1N1B4_1475at 1692.347 kb on + strand, within Pf1N1B4_1475at 1692.347 kb on + strand, within Pf1N1B4_1475at 1692.347 kb on + strand, within Pf1N1B4_1475at 1692.347 kb on + strand, within Pf1N1B4_1475at 1692.347 kb on + strand, within Pf1N1B4_1475at 1692.347 kb on + strand, within Pf1N1B4_1475at 1692.347 kb on + strand, within Pf1N1B4_1475at 1692.347 kb on + strand, within Pf1N1B4_1475at 1692.348 kb on - strand, within Pf1N1B4_1475at 1692.370 kb on + strand, within Pf1N1B4_1475at 1692.370 kb on + strand, within Pf1N1B4_1475at 1692.370 kb on + strand, within Pf1N1B4_1475at 1692.370 kb on + strand, within Pf1N1B4_1475at 1692.370 kb on + strand, within Pf1N1B4_1475at 1692.796 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Mannose (C)
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1,688,835 - -1.3
1,688,922 - +1.6
1,688,922 - -0.7
1,688,922 - +0.3
1,688,922 - +0.6
1,688,925 - -2.3
1,689,194 - -0.7
1,689,248 + +1.6
1,689,249 - +0.3
1,689,249 - +1.3
1,689,310 + +1.6
1,689,310 + -1.1
1,689,311 - -0.5
1,689,311 - +0.8
1,689,378 - -0.7
1,689,387 + +0.3
1,689,405 + -2.7
1,689,749 + Pf1N1B4_1474 0.21 +0.3
1,689,901 - Pf1N1B4_1474 0.27 -2.1
1,689,901 - Pf1N1B4_1474 0.27 -0.7
1,689,993 - Pf1N1B4_1474 0.30 -1.3
1,690,377 - Pf1N1B4_1474 0.43 +0.3
1,690,739 + Pf1N1B4_1474 0.55 +0.3
1,690,785 + Pf1N1B4_1474 0.57 +0.3
1,690,786 - Pf1N1B4_1474 0.57 -1.3
1,690,957 + Pf1N1B4_1474 0.62 +0.3
1,690,958 - Pf1N1B4_1474 0.62 -2.3
1,690,960 + Pf1N1B4_1474 0.63 -0.9
1,690,961 - Pf1N1B4_1474 0.63 +0.3
1,691,268 - Pf1N1B4_1474 0.73 -2.1
1,691,268 - Pf1N1B4_1474 0.73 -0.7
1,691,271 - Pf1N1B4_1474 0.73 -1.5
1,691,271 - Pf1N1B4_1474 0.73 -1.1
1,691,271 - Pf1N1B4_1474 0.73 -0.7
1,691,603 + Pf1N1B4_1474 0.84 -1.7
1,691,603 + Pf1N1B4_1474 0.84 -0.7
1,691,604 - Pf1N1B4_1474 0.84 -0.1
1,691,604 - Pf1N1B4_1474 0.84 -1.3
1,691,604 - Pf1N1B4_1474 0.84 -1.3
1,691,604 - Pf1N1B4_1474 0.84 +0.3
1,692,026 - +1.0
1,692,026 - -0.1
1,692,066 - +0.6
1,692,096 + -0.3
1,692,096 + +1.1
1,692,097 - -1.0
1,692,097 - +0.5
1,692,097 - +2.3
1,692,228 + +0.3
1,692,252 + -2.3
1,692,275 + -1.1
1,692,288 + +1.6
1,692,347 + Pf1N1B4_1475 0.40 -1.3
1,692,347 + Pf1N1B4_1475 0.40 -0.7
1,692,347 + Pf1N1B4_1475 0.40 -0.5
1,692,347 + Pf1N1B4_1475 0.40 -0.5
1,692,347 + Pf1N1B4_1475 0.40 -0.5
1,692,347 + Pf1N1B4_1475 0.40 -1.3
1,692,347 + Pf1N1B4_1475 0.40 -0.3
1,692,347 + Pf1N1B4_1475 0.40 -1.3
1,692,347 + Pf1N1B4_1475 0.40 -1.1
1,692,347 + Pf1N1B4_1475 0.40 +1.1
1,692,347 + Pf1N1B4_1475 0.40 +0.7
1,692,347 + Pf1N1B4_1475 0.40 -1.1
1,692,347 + Pf1N1B4_1475 0.40 +0.4
1,692,347 + Pf1N1B4_1475 0.40 +0.3
1,692,348 - Pf1N1B4_1475 0.41 +0.7
1,692,370 + Pf1N1B4_1475 0.53 -0.7
1,692,370 + Pf1N1B4_1475 0.53 -0.7
1,692,370 + Pf1N1B4_1475 0.53 +0.9
1,692,370 + Pf1N1B4_1475 0.53 -0.1
1,692,370 + Pf1N1B4_1475 0.53 +0.6
1,692,796 + +0.3

Or see this region's nucleotide sequence