Experiment: spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3320 and Dsui_3321 are separated by 153 nucleotides Dsui_3321 and Dsui_3322 are separated by 132 nucleotides Dsui_3322 and Dsui_3323 are separated by 137 nucleotides
Dsui_3320: Dsui_3320 - 1,4-dihydroxy-2-naphthoate octaprenyltransferase, at 3,560,493 to 3,561,413
_3320
Dsui_3321: Dsui_3321 - tRNA-Leu, at 3,561,567 to 3,561,653
_3321
Dsui_3322: Dsui_3322 - S-adenosylmethionine:tRNA ribosyltransferase-isomerase, at 3,561,786 to 3,562,823
_3322
Dsui_3323: Dsui_3323 - tRNA-guanine transglycosylase, queuosine-34-forming, at 3,562,961 to 3,564,085
_3323
Position (kb)
3561
3562
3563 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3560.789 kb on + strand, within Dsui_3320 at 3560.830 kb on + strand, within Dsui_3320 at 3560.877 kb on - strand, within Dsui_3320 at 3560.966 kb on - strand, within Dsui_3320 at 3561.086 kb on + strand, within Dsui_3320 at 3561.141 kb on + strand at 3561.144 kb on - strand, within Dsui_3320 at 3561.152 kb on + strand, within Dsui_3320 at 3561.161 kb on + strand, within Dsui_3320 at 3561.199 kb on + strand at 3561.209 kb on + strand, within Dsui_3320 at 3561.217 kb on - strand, within Dsui_3320 at 3561.217 kb on - strand, within Dsui_3320 at 3561.437 kb on - strand at 3561.481 kb on + strand at 3561.489 kb on - strand at 3561.490 kb on - strand at 3561.524 kb on - strand at 3561.524 kb on - strand at 3561.665 kb on + strand at 3561.686 kb on - strand at 3561.768 kb on + strand at 3561.847 kb on + strand at 3561.889 kb on + strand at 3561.889 kb on + strand at 3561.921 kb on - strand at 3561.948 kb on + strand, within Dsui_3322 at 3562.016 kb on + strand, within Dsui_3322 at 3562.120 kb on + strand, within Dsui_3322 at 3562.260 kb on + strand, within Dsui_3322 at 3562.281 kb on + strand, within Dsui_3322 at 3562.419 kb on - strand at 3562.546 kb on - strand at 3562.558 kb on + strand, within Dsui_3322 at 3562.558 kb on + strand, within Dsui_3322 at 3562.684 kb on + strand at 3562.684 kb on + strand at 3562.684 kb on + strand, within Dsui_3322 at 3562.692 kb on - strand at 3562.695 kb on - strand at 3562.740 kb on + strand at 3562.740 kb on + strand at 3562.784 kb on + strand at 3562.824 kb on - strand at 3562.847 kb on + strand at 3562.847 kb on + strand at 3562.855 kb on - strand at 3562.877 kb on + strand at 3562.885 kb on - strand at 3562.888 kb on + strand at 3562.956 kb on + strand at 3562.956 kb on + strand at 3562.956 kb on + strand at 3562.956 kb on + strand at 3562.975 kb on + strand at 3563.030 kb on + strand at 3563.061 kb on + strand at 3563.061 kb on + strand at 3563.069 kb on - strand at 3563.111 kb on + strand, within Dsui_3323 at 3563.206 kb on + strand, within Dsui_3323 at 3563.263 kb on + strand, within Dsui_3323 at 3563.282 kb on + strand, within Dsui_3323 at 3563.633 kb on + strand, within Dsui_3323 at 3563.788 kb on + strand at 3563.789 kb on + strand, within Dsui_3323
Per-strain Table
Position Strand Gene LocusTag Fraction spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 3,560,789 + Dsui_3320 0.32 +0.6 3,560,830 + Dsui_3320 0.37 +0.2 3,560,877 - Dsui_3320 0.42 +1.4 3,560,966 - Dsui_3320 0.51 +0.9 3,561,086 + Dsui_3320 0.64 +0.7 3,561,141 + +1.8 3,561,144 - Dsui_3320 0.71 +1.5 3,561,152 + Dsui_3320 0.72 -1.5 3,561,161 + Dsui_3320 0.73 +0.6 3,561,199 + +0.5 3,561,209 + Dsui_3320 0.78 -0.6 3,561,217 - Dsui_3320 0.79 +1.3 3,561,217 - Dsui_3320 0.79 +1.2 3,561,437 - +0.1 3,561,481 + +0.1 3,561,489 - +0.3 3,561,490 - -0.3 3,561,524 - +1.2 3,561,524 - +0.8 3,561,665 + -2.0 3,561,686 - +0.3 3,561,768 + -1.4 3,561,847 + -0.5 3,561,889 + +0.1 3,561,889 + -0.3 3,561,921 - -0.6 3,561,948 + Dsui_3322 0.16 -2.0 3,562,016 + Dsui_3322 0.22 -0.0 3,562,120 + Dsui_3322 0.32 -2.6 3,562,260 + Dsui_3322 0.46 +0.2 3,562,281 + Dsui_3322 0.48 +0.1 3,562,419 - -3.6 3,562,546 - -2.3 3,562,558 + Dsui_3322 0.74 +0.2 3,562,558 + Dsui_3322 0.74 -1.5 3,562,684 + +0.6 3,562,684 + -2.6 3,562,684 + Dsui_3322 0.87 +0.1 3,562,692 - +2.0 3,562,695 - -3.6 3,562,740 + -0.3 3,562,740 + +1.2 3,562,784 + +1.1 3,562,824 - -0.5 3,562,847 + +0.8 3,562,847 + +1.0 3,562,855 - -2.1 3,562,877 + +0.3 3,562,885 - -1.6 3,562,888 + +0.6 3,562,956 + +1.2 3,562,956 + +0.3 3,562,956 + +0.2 3,562,956 + +0.4 3,562,975 + -2.3 3,563,030 + -1.2 3,563,061 + -1.0 3,563,061 + -3.8 3,563,069 - -0.1 3,563,111 + Dsui_3323 0.13 -1.2 3,563,206 + Dsui_3323 0.22 -1.2 3,563,263 + Dsui_3323 0.27 -0.8 3,563,282 + Dsui_3323 0.29 -3.5 3,563,633 + Dsui_3323 0.60 -1.0 3,563,788 + -1.4 3,563,789 + Dsui_3323 0.74 -0.4
Or see this region's nucleotide sequence