Experiment: spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2654 and Dsui_2655 overlap by 4 nucleotides Dsui_2655 and Dsui_2656 are separated by 13 nucleotides Dsui_2656 and Dsui_2657 are separated by 234 nucleotides
Dsui_2654: Dsui_2654 - DNA polymerase III, delta'' subunit, at 2,809,403 to 2,810,428
_2654
Dsui_2655: Dsui_2655 - thymidylate kinase, at 2,810,425 to 2,811,036
_2655
Dsui_2656: Dsui_2656 - conserved hypothetical protein, YceG family, at 2,811,050 to 2,812,045
_2656
Dsui_2657: Dsui_2657 - folate-binding protein YgfZ, at 2,812,280 to 2,813,299
_2657
Position (kb)
2811
2812
2813 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2811.094 kb on + strand at 2811.250 kb on + strand at 2811.250 kb on - strand, within Dsui_2656 at 2811.265 kb on + strand, within Dsui_2656 at 2811.273 kb on - strand, within Dsui_2656 at 2811.327 kb on + strand, within Dsui_2656 at 2811.434 kb on + strand, within Dsui_2656 at 2811.446 kb on - strand, within Dsui_2656 at 2811.537 kb on - strand, within Dsui_2656 at 2811.570 kb on + strand at 2811.658 kb on + strand at 2811.665 kb on - strand, within Dsui_2656 at 2811.666 kb on - strand, within Dsui_2656 at 2811.711 kb on + strand at 2811.792 kb on - strand, within Dsui_2656 at 2811.831 kb on + strand, within Dsui_2656 at 2811.917 kb on - strand, within Dsui_2656 at 2811.975 kb on - strand at 2812.003 kb on - strand at 2812.070 kb on - strand at 2812.089 kb on - strand at 2812.139 kb on + strand at 2812.154 kb on + strand at 2812.253 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 2,811,094 + +4.5 2,811,250 + +2.0 2,811,250 - Dsui_2656 0.20 +0.2 2,811,265 + Dsui_2656 0.22 -2.7 2,811,273 - Dsui_2656 0.22 +0.7 2,811,327 + Dsui_2656 0.28 -2.2 2,811,434 + Dsui_2656 0.39 -1.4 2,811,446 - Dsui_2656 0.40 -2.4 2,811,537 - Dsui_2656 0.49 -0.0 2,811,570 + +1.3 2,811,658 + -1.7 2,811,665 - Dsui_2656 0.62 +0.3 2,811,666 - Dsui_2656 0.62 +1.2 2,811,711 + -2.2 2,811,792 - Dsui_2656 0.74 +0.4 2,811,831 + Dsui_2656 0.78 -3.7 2,811,917 - Dsui_2656 0.87 -0.3 2,811,975 - -0.2 2,812,003 - +0.3 2,812,070 - +0.8 2,812,089 - -1.5 2,812,139 + +0.6 2,812,154 + +1.4 2,812,253 - -3.1
Or see this region's nucleotide sequence