Experiment: spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2116 and Dsui_2117 are separated by 160 nucleotides Dsui_2117 and Dsui_2118 are separated by 97 nucleotides
Dsui_2116: Dsui_2116 - Fe-dependent oxidoreductase, alcohol dehydrogenase, at 2,241,610 to 2,242,758
_2116
Dsui_2117: Dsui_2117 - acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit, at 2,242,919 to 2,243,785
_2117
Dsui_2118: Dsui_2118 - acyl CoA:acetate/3-ketoacid CoA transferase, alpha subunit, at 2,243,883 to 2,244,890
_2118
Position (kb)
2242
2243
2244 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2242.053 kb on + strand at 2242.133 kb on - strand, within Dsui_2116 at 2242.330 kb on + strand, within Dsui_2116 at 2242.377 kb on + strand, within Dsui_2116 at 2242.406 kb on + strand at 2242.532 kb on - strand at 2242.580 kb on - strand at 2242.656 kb on + strand at 2242.668 kb on + strand at 2242.705 kb on + strand at 2242.705 kb on + strand at 2242.758 kb on + strand at 2242.761 kb on - strand at 2242.771 kb on + strand at 2242.781 kb on + strand at 2242.813 kb on - strand at 2242.895 kb on + strand at 2242.973 kb on - strand at 2243.024 kb on - strand at 2243.024 kb on - strand, within Dsui_2117 at 2243.076 kb on - strand at 2243.076 kb on - strand, within Dsui_2117 at 2243.270 kb on - strand, within Dsui_2117 at 2243.331 kb on + strand at 2243.337 kb on - strand at 2243.396 kb on - strand at 2243.396 kb on - strand, within Dsui_2117 at 2243.537 kb on - strand at 2243.712 kb on + strand at 2243.875 kb on - strand at 2243.929 kb on + strand at 2243.937 kb on - strand at 2244.115 kb on + strand at 2244.278 kb on - strand at 2244.310 kb on - strand at 2244.385 kb on + strand at 2244.583 kb on - strand, within Dsui_2118 at 2244.616 kb on + strand at 2244.673 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 2,242,053 + -3.4 2,242,133 - Dsui_2116 0.46 -0.6 2,242,330 + Dsui_2116 0.63 -0.7 2,242,377 + Dsui_2116 0.67 -1.0 2,242,406 + +0.3 2,242,532 - +2.0 2,242,580 - -1.3 2,242,656 + +0.2 2,242,668 + -2.8 2,242,705 + +1.2 2,242,705 + -0.6 2,242,758 + +0.5 2,242,761 - -1.3 2,242,771 + -0.7 2,242,781 + -2.3 2,242,813 - -0.3 2,242,895 + +0.2 2,242,973 - -0.8 2,243,024 - -2.1 2,243,024 - Dsui_2117 0.12 +0.3 2,243,076 - +1.3 2,243,076 - Dsui_2117 0.18 -1.5 2,243,270 - Dsui_2117 0.40 -2.5 2,243,331 + -3.8 2,243,337 - -1.7 2,243,396 - -2.4 2,243,396 - Dsui_2117 0.55 -3.3 2,243,537 - -1.5 2,243,712 + -2.4 2,243,875 - -0.4 2,243,929 + -0.7 2,243,937 - +0.3 2,244,115 + -0.1 2,244,278 - -1.5 2,244,310 - +0.0 2,244,385 + -1.8 2,244,583 - Dsui_2118 0.69 -1.9 2,244,616 + -1.2 2,244,673 + -2.4
Or see this region's nucleotide sequence