Experiment: spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1711 and Dsui_1712 overlap by 26 nucleotides Dsui_1712 and Dsui_1713 are separated by 6 nucleotides Dsui_1713 and Dsui_1714 are separated by 68 nucleotides Dsui_1714 and Dsui_1715 are separated by 14 nucleotides
Dsui_1711: Dsui_1711 - methylase of chemotaxis methyl-accepting protein, at 1,800,408 to 1,801,286
_1711
Dsui_1712: Dsui_1712 - chemotaxis protein, at 1,801,261 to 1,801,908
_1712
Dsui_1713: Dsui_1713 - chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain, at 1,801,915 to 1,802,940
_1713
Dsui_1714: Dsui_1714 - anti-anti-sigma factor, at 1,803,009 to 1,803,314
_1714
Dsui_1715: Dsui_1715 - response regulator with CheY-like receiver domain and winged-helix DNA-binding domain, at 1,803,329 to 1,805,083
_1715
Position (kb)
1801
1802
1803 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1800.959 kb on + strand, within Dsui_1711 at 1800.967 kb on - strand, within Dsui_1711 at 1800.999 kb on + strand, within Dsui_1711 at 1800.999 kb on + strand, within Dsui_1711 at 1800.999 kb on + strand, within Dsui_1711 at 1800.999 kb on + strand, within Dsui_1711 at 1801.007 kb on - strand, within Dsui_1711 at 1801.193 kb on - strand, within Dsui_1711 at 1801.267 kb on - strand at 1801.395 kb on - strand, within Dsui_1712 at 1801.511 kb on - strand, within Dsui_1712 at 1801.527 kb on + strand, within Dsui_1712 at 1801.611 kb on + strand, within Dsui_1712 at 1801.780 kb on - strand, within Dsui_1712 at 1801.823 kb on + strand, within Dsui_1712 at 1801.861 kb on + strand at 1801.869 kb on - strand at 1801.905 kb on - strand at 1801.917 kb on + strand at 1801.955 kb on + strand at 1802.076 kb on + strand, within Dsui_1713 at 1802.110 kb on - strand, within Dsui_1713 at 1802.110 kb on - strand, within Dsui_1713 at 1802.238 kb on + strand, within Dsui_1713 at 1802.278 kb on - strand, within Dsui_1713 at 1802.579 kb on - strand, within Dsui_1713 at 1802.635 kb on - strand, within Dsui_1713 at 1802.655 kb on + strand, within Dsui_1713 at 1802.692 kb on + strand, within Dsui_1713 at 1802.740 kb on - strand, within Dsui_1713 at 1802.834 kb on + strand, within Dsui_1713 at 1802.847 kb on - strand at 1802.863 kb on + strand at 1802.977 kb on + strand at 1802.983 kb on - strand at 1802.983 kb on - strand at 1803.011 kb on + strand at 1803.019 kb on + strand at 1803.019 kb on + strand at 1803.019 kb on + strand at 1803.024 kb on + strand at 1803.027 kb on - strand at 1803.027 kb on - strand at 1803.027 kb on - strand at 1803.029 kb on + strand at 1803.099 kb on + strand, within Dsui_1714 at 1803.099 kb on + strand, within Dsui_1714 at 1803.103 kb on + strand, within Dsui_1714 at 1803.107 kb on - strand, within Dsui_1714 at 1803.138 kb on + strand, within Dsui_1714 at 1803.147 kb on + strand, within Dsui_1714 at 1803.194 kb on - strand, within Dsui_1714 at 1803.198 kb on - strand at 1803.205 kb on + strand, within Dsui_1714 at 1803.219 kb on - strand at 1803.259 kb on + strand, within Dsui_1714 at 1803.267 kb on - strand, within Dsui_1714 at 1803.319 kb on + strand at 1803.319 kb on + strand at 1803.319 kb on + strand at 1803.471 kb on + strand at 1803.478 kb on - strand at 1803.684 kb on + strand, within Dsui_1715 at 1803.799 kb on - strand, within Dsui_1715 at 1803.799 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 1,800,959 + Dsui_1711 0.63 +0.6 1,800,967 - Dsui_1711 0.64 -0.0 1,800,999 + Dsui_1711 0.67 +0.0 1,800,999 + Dsui_1711 0.67 +1.0 1,800,999 + Dsui_1711 0.67 +1.3 1,800,999 + Dsui_1711 0.67 +0.8 1,801,007 - Dsui_1711 0.68 -0.3 1,801,193 - Dsui_1711 0.89 -0.4 1,801,267 - +0.3 1,801,395 - Dsui_1712 0.21 -1.3 1,801,511 - Dsui_1712 0.39 +1.1 1,801,527 + Dsui_1712 0.41 +0.6 1,801,611 + Dsui_1712 0.54 +0.2 1,801,780 - Dsui_1712 0.80 +1.9 1,801,823 + Dsui_1712 0.87 -1.2 1,801,861 + +0.6 1,801,869 - -1.8 1,801,905 - -1.8 1,801,917 + +0.8 1,801,955 + -0.0 1,802,076 + Dsui_1713 0.16 +0.2 1,802,110 - Dsui_1713 0.19 -3.3 1,802,110 - Dsui_1713 0.19 -0.1 1,802,238 + Dsui_1713 0.31 +0.6 1,802,278 - Dsui_1713 0.35 -1.5 1,802,579 - Dsui_1713 0.65 -0.6 1,802,635 - Dsui_1713 0.70 -0.6 1,802,655 + Dsui_1713 0.72 +0.2 1,802,692 + Dsui_1713 0.76 -0.1 1,802,740 - Dsui_1713 0.80 -0.2 1,802,834 + Dsui_1713 0.90 +0.1 1,802,847 - -0.4 1,802,863 + +1.3 1,802,977 + +1.1 1,802,983 - +0.8 1,802,983 - -3.8 1,803,011 + +1.2 1,803,019 + +0.5 1,803,019 + +0.5 1,803,019 + +1.1 1,803,024 + +0.4 1,803,027 - +0.6 1,803,027 - +2.3 1,803,027 - -2.0 1,803,029 + +1.2 1,803,099 + Dsui_1714 0.29 +0.9 1,803,099 + Dsui_1714 0.29 +0.8 1,803,103 + Dsui_1714 0.31 +0.7 1,803,107 - Dsui_1714 0.32 -2.0 1,803,138 + Dsui_1714 0.42 -2.8 1,803,147 + Dsui_1714 0.45 +0.2 1,803,194 - Dsui_1714 0.60 +0.5 1,803,198 - +0.9 1,803,205 + Dsui_1714 0.64 -0.2 1,803,219 - -2.3 1,803,259 + Dsui_1714 0.82 +0.6 1,803,267 - Dsui_1714 0.84 +0.6 1,803,319 + +0.8 1,803,319 + +3.3 1,803,319 + +1.2 1,803,471 + +0.8 1,803,478 - -2.9 1,803,684 + Dsui_1715 0.20 +0.9 1,803,799 - Dsui_1715 0.27 -4.5 1,803,799 - -0.9
Or see this region's nucleotide sequence