Experiment: spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1523 and Dsui_1524 are separated by 64 nucleotides Dsui_1524 and Dsui_1525 are separated by 87 nucleotides Dsui_1525 and Dsui_1526 are separated by 73 nucleotides Dsui_1526 and Dsui_1527 are separated by 635 nucleotides
Dsui_1523: Dsui_1523 - drug resistance transporter, EmrB/QacA subfamily, at 1,585,476 to 1,586,927
_1523
Dsui_1524: Dsui_1524 - transcriptional regulator, at 1,586,992 to 1,587,495
_1524
Dsui_1525: Dsui_1525 - sulfate/thiosulfate-binding protein, at 1,587,583 to 1,588,590
_1525
Dsui_1526: Dsui_1526 - hypothetical protein, at 1,588,664 to 1,588,861
_1526
Dsui_1527: Dsui_1527 - hypothetical protein, at 1,589,497 to 1,589,706
_1527
Position (kb)
1587
1588
1589 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1586.644 kb on + strand at 1586.692 kb on - strand at 1586.751 kb on + strand, within Dsui_1523 at 1586.759 kb on - strand, within Dsui_1523 at 1586.774 kb on + strand at 1586.838 kb on + strand at 1586.846 kb on - strand at 1586.902 kb on - strand at 1586.936 kb on - strand at 1587.079 kb on + strand, within Dsui_1524 at 1587.079 kb on + strand, within Dsui_1524 at 1587.105 kb on + strand, within Dsui_1524 at 1587.189 kb on + strand, within Dsui_1524 at 1587.341 kb on + strand, within Dsui_1524 at 1587.351 kb on - strand, within Dsui_1524 at 1587.351 kb on - strand at 1587.351 kb on - strand at 1587.383 kb on + strand, within Dsui_1524 at 1587.434 kb on + strand, within Dsui_1524 at 1587.541 kb on + strand at 1587.549 kb on - strand at 1587.549 kb on - strand at 1587.549 kb on - strand at 1587.549 kb on - strand at 1587.549 kb on - strand at 1587.549 kb on - strand at 1587.587 kb on - strand at 1587.651 kb on - strand at 1587.677 kb on - strand at 1587.692 kb on + strand, within Dsui_1525 at 1587.700 kb on - strand, within Dsui_1525 at 1587.700 kb on - strand, within Dsui_1525 at 1587.719 kb on - strand, within Dsui_1525 at 1587.757 kb on + strand, within Dsui_1525 at 1587.772 kb on - strand, within Dsui_1525 at 1587.839 kb on + strand, within Dsui_1525 at 1587.839 kb on + strand, within Dsui_1525 at 1587.923 kb on - strand, within Dsui_1525 at 1587.928 kb on + strand, within Dsui_1525 at 1587.964 kb on + strand, within Dsui_1525 at 1588.041 kb on - strand at 1588.148 kb on - strand at 1588.169 kb on - strand, within Dsui_1525 at 1588.211 kb on - strand, within Dsui_1525 at 1588.340 kb on - strand, within Dsui_1525 at 1588.376 kb on - strand, within Dsui_1525 at 1588.389 kb on + strand, within Dsui_1525 at 1588.389 kb on + strand, within Dsui_1525 at 1588.389 kb on + strand, within Dsui_1525 at 1588.507 kb on - strand at 1588.519 kb on + strand at 1588.519 kb on + strand at 1588.536 kb on - strand at 1588.571 kb on - strand at 1588.572 kb on + strand at 1588.572 kb on + strand at 1588.655 kb on + strand at 1588.726 kb on + strand, within Dsui_1526 at 1588.726 kb on + strand at 1588.734 kb on - strand, within Dsui_1526 at 1588.754 kb on + strand, within Dsui_1526 at 1588.782 kb on - strand, within Dsui_1526 at 1588.803 kb on - strand, within Dsui_1526 at 1588.803 kb on - strand, within Dsui_1526 at 1588.831 kb on + strand, within Dsui_1526 at 1588.870 kb on + strand at 1588.892 kb on - strand at 1588.922 kb on - strand at 1588.922 kb on - strand at 1589.161 kb on - strand at 1589.163 kb on + strand at 1589.164 kb on - strand at 1589.203 kb on + strand at 1589.218 kb on - strand at 1589.275 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 1,586,644 + -3.6 1,586,692 - -1.4 1,586,751 + Dsui_1523 0.88 -3.8 1,586,759 - Dsui_1523 0.88 +0.9 1,586,774 + -2.3 1,586,838 + -0.4 1,586,846 - -0.1 1,586,902 - -0.2 1,586,936 - -1.3 1,587,079 + Dsui_1524 0.17 +1.8 1,587,079 + Dsui_1524 0.17 +1.3 1,587,105 + Dsui_1524 0.22 +0.7 1,587,189 + Dsui_1524 0.39 +0.6 1,587,341 + Dsui_1524 0.69 +1.9 1,587,351 - Dsui_1524 0.71 -0.7 1,587,351 - -0.7 1,587,351 - -0.2 1,587,383 + Dsui_1524 0.78 +0.7 1,587,434 + Dsui_1524 0.88 +0.8 1,587,541 + -1.2 1,587,549 - -3.5 1,587,549 - -3.4 1,587,549 - -2.7 1,587,549 - -1.9 1,587,549 - -0.8 1,587,549 - -0.5 1,587,587 - -2.4 1,587,651 - -0.2 1,587,677 - -3.4 1,587,692 + Dsui_1525 0.11 -3.6 1,587,700 - Dsui_1525 0.12 -2.6 1,587,700 - Dsui_1525 0.12 -2.6 1,587,719 - Dsui_1525 0.13 -0.9 1,587,757 + Dsui_1525 0.17 +0.3 1,587,772 - Dsui_1525 0.19 -1.9 1,587,839 + Dsui_1525 0.25 -1.2 1,587,839 + Dsui_1525 0.25 -1.0 1,587,923 - Dsui_1525 0.34 -1.9 1,587,928 + Dsui_1525 0.34 -0.2 1,587,964 + Dsui_1525 0.38 +0.6 1,588,041 - -3.1 1,588,148 - -1.0 1,588,169 - Dsui_1525 0.58 -4.1 1,588,211 - Dsui_1525 0.62 -1.7 1,588,340 - Dsui_1525 0.75 -0.8 1,588,376 - Dsui_1525 0.79 -1.9 1,588,389 + Dsui_1525 0.80 -0.9 1,588,389 + Dsui_1525 0.80 -1.3 1,588,389 + Dsui_1525 0.80 -1.7 1,588,507 - -3.8 1,588,519 + -3.5 1,588,519 + +0.6 1,588,536 - -1.8 1,588,571 - -2.4 1,588,572 + +0.4 1,588,572 + +1.3 1,588,655 + -1.9 1,588,726 + Dsui_1526 0.31 -3.1 1,588,726 + -1.0 1,588,734 - Dsui_1526 0.35 -3.0 1,588,754 + Dsui_1526 0.45 +0.2 1,588,782 - Dsui_1526 0.60 +0.1 1,588,803 - Dsui_1526 0.70 +0.9 1,588,803 - Dsui_1526 0.70 +0.4 1,588,831 + Dsui_1526 0.84 +0.4 1,588,870 + -0.3 1,588,892 - +0.9 1,588,922 - +0.5 1,588,922 - -0.3 1,589,161 - -0.4 1,589,163 + +1.1 1,589,164 - +2.1 1,589,203 + +0.9 1,589,218 - +1.5 1,589,275 - +0.8
Or see this region's nucleotide sequence