Experiment: spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0972 and Dsui_0973 are separated by 236 nucleotides Dsui_0973 and Dsui_0974 are separated by 85 nucleotides Dsui_0974 and Dsui_0975 are separated by 150 nucleotides
Dsui_0972: Dsui_0972 - Zn-dependent hydrolase, glyoxylase, at 998,688 to 999,722
_0972
Dsui_0973: Dsui_0973 - putative transcriptional regulator, at 999,959 to 1,000,429
_0973
Dsui_0974: Dsui_0974 - hypothetical protein, at 1,000,515 to 1,000,979
_0974
Dsui_0975: Dsui_0975 - acyl-CoA dehydrogenase, at 1,001,130 to 1,002,302
_0975
Position (kb) 1000
1001 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 999.632 kb on - strand at 999.635 kb on + strand at 999.643 kb on - strand at 999.662 kb on + strand at 999.670 kb on - strand at 999.676 kb on - strand at 999.724 kb on + strand at 999.728 kb on + strand at 999.728 kb on + strand at 999.728 kb on + strand at 999.736 kb on - strand at 999.811 kb on + strand at 999.834 kb on - strand at 999.899 kb on + strand at 999.989 kb on + strand at 1000.006 kb on - strand, within Dsui_0973 at 1000.031 kb on - strand at 1000.046 kb on - strand, within Dsui_0973 at 1000.069 kb on + strand, within Dsui_0973 at 1000.119 kb on - strand, within Dsui_0973 at 1000.119 kb on - strand, within Dsui_0973 at 1000.172 kb on - strand, within Dsui_0973 at 1000.230 kb on - strand, within Dsui_0973 at 1000.308 kb on - strand, within Dsui_0973 at 1000.363 kb on + strand, within Dsui_0973 at 1000.383 kb on - strand at 1000.682 kb on + strand, within Dsui_0974 at 1000.806 kb on + strand, within Dsui_0974 at 1000.904 kb on + strand, within Dsui_0974 at 1000.919 kb on - strand, within Dsui_0974 at 1000.974 kb on + strand at 1000.982 kb on - strand at 1001.049 kb on - strand at 1001.049 kb on - strand at 1001.049 kb on - strand at 1001.069 kb on + strand at 1001.083 kb on + strand at 1001.091 kb on - strand at 1001.101 kb on - strand at 1001.151 kb on - strand at 1001.180 kb on + strand at 1001.213 kb on - strand at 1001.234 kb on + strand at 1001.331 kb on + strand, within Dsui_0975 at 1001.331 kb on + strand, within Dsui_0975 at 1001.339 kb on - strand at 1001.351 kb on + strand, within Dsui_0975 at 1001.421 kb on - strand, within Dsui_0975 at 1001.466 kb on + strand, within Dsui_0975 at 1001.516 kb on - strand, within Dsui_0975 at 1001.566 kb on - strand, within Dsui_0975 at 1001.578 kb on - strand, within Dsui_0975 at 1001.651 kb on + strand, within Dsui_0975 at 1001.746 kb on + strand, within Dsui_0975 at 1001.770 kb on + strand, within Dsui_0975 at 1001.954 kb on + strand, within Dsui_0975
Per-strain Table
Position Strand Gene LocusTag Fraction spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 999,632 - -0.2 999,635 + +0.5 999,643 - +1.3 999,662 + +0.8 999,670 - +1.5 999,676 - +1.2 999,724 + +0.5 999,728 + -0.5 999,728 + -0.1 999,728 + -0.9 999,736 - +0.1 999,811 + +0.4 999,834 - +1.0 999,899 + -0.1 999,989 + +0.6 1,000,006 - Dsui_0973 0.10 -3.0 1,000,031 - -2.4 1,000,046 - Dsui_0973 0.18 -1.4 1,000,069 + Dsui_0973 0.23 +0.8 1,000,119 - Dsui_0973 0.34 -0.3 1,000,119 - Dsui_0973 0.34 -2.9 1,000,172 - Dsui_0973 0.45 -1.0 1,000,230 - Dsui_0973 0.58 -0.7 1,000,308 - Dsui_0973 0.74 -2.5 1,000,363 + Dsui_0973 0.86 +1.4 1,000,383 - -2.6 1,000,682 + Dsui_0974 0.36 +1.2 1,000,806 + Dsui_0974 0.63 +0.1 1,000,904 + Dsui_0974 0.84 +0.9 1,000,919 - Dsui_0974 0.87 +0.2 1,000,974 + +0.7 1,000,982 - -1.0 1,001,049 - +0.3 1,001,049 - +0.9 1,001,049 - -0.4 1,001,069 + +0.4 1,001,083 + +0.8 1,001,091 - -0.8 1,001,101 - -1.2 1,001,151 - -1.2 1,001,180 + +0.3 1,001,213 - -1.0 1,001,234 + +1.4 1,001,331 + Dsui_0975 0.17 +0.4 1,001,331 + Dsui_0975 0.17 +0.6 1,001,339 - -2.2 1,001,351 + Dsui_0975 0.19 -0.1 1,001,421 - Dsui_0975 0.25 +0.0 1,001,466 + Dsui_0975 0.29 +0.2 1,001,516 - Dsui_0975 0.33 +0.0 1,001,566 - Dsui_0975 0.37 +0.5 1,001,578 - Dsui_0975 0.38 +3.1 1,001,651 + Dsui_0975 0.44 +0.6 1,001,746 + Dsui_0975 0.53 +0.1 1,001,770 + Dsui_0975 0.55 +0.5 1,001,954 + Dsui_0975 0.70 +0.3
Or see this region's nucleotide sequence