Experiment: spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0969 and Dsui_0970 are separated by 139 nucleotides Dsui_0970 and Dsui_0971 are separated by 73 nucleotides
Dsui_0969: Dsui_0969 - putative AP superfamily protein, at 994,177 to 995,337
_0969
Dsui_0970: Dsui_0970 - PAS domain S-box/diguanylate cyclase (GGDEF) domain-containing protein, at 995,477 to 996,766
_0970
Dsui_0971: Dsui_0971 - poly(R)-hydroxyalkanoic acid synthase, class I, at 996,840 to 998,669
_0971
Position (kb)
995
996
997 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 994.575 kb on + strand, within Dsui_0969 at 994.617 kb on + strand, within Dsui_0969 at 994.674 kb on - strand, within Dsui_0969 at 994.723 kb on + strand, within Dsui_0969 at 994.731 kb on - strand, within Dsui_0969 at 994.793 kb on + strand, within Dsui_0969 at 994.831 kb on + strand, within Dsui_0969 at 994.839 kb on - strand, within Dsui_0969 at 994.875 kb on - strand, within Dsui_0969 at 994.979 kb on + strand, within Dsui_0969 at 994.999 kb on - strand, within Dsui_0969 at 995.144 kb on + strand, within Dsui_0969 at 995.152 kb on - strand at 995.245 kb on + strand at 995.263 kb on - strand at 995.299 kb on - strand at 995.475 kb on + strand at 995.613 kb on + strand, within Dsui_0970 at 995.645 kb on + strand, within Dsui_0970 at 995.689 kb on + strand, within Dsui_0970 at 996.113 kb on + strand, within Dsui_0970 at 996.135 kb on + strand, within Dsui_0970 at 996.144 kb on - strand, within Dsui_0970 at 996.230 kb on - strand at 996.247 kb on - strand, within Dsui_0970 at 996.299 kb on - strand, within Dsui_0970 at 996.307 kb on - strand, within Dsui_0970 at 996.312 kb on - strand, within Dsui_0970 at 996.321 kb on + strand, within Dsui_0970 at 996.422 kb on + strand, within Dsui_0970 at 996.576 kb on + strand, within Dsui_0970 at 996.595 kb on - strand, within Dsui_0970 at 996.727 kb on + strand at 997.319 kb on + strand, within Dsui_0971 at 997.319 kb on + strand, within Dsui_0971 at 997.327 kb on - strand, within Dsui_0971 at 997.327 kb on - strand, within Dsui_0971 at 997.327 kb on - strand, within Dsui_0971 at 997.327 kb on - strand, within Dsui_0971 at 997.327 kb on - strand, within Dsui_0971 at 997.327 kb on - strand, within Dsui_0971 at 997.327 kb on - strand, within Dsui_0971 at 997.327 kb on - strand, within Dsui_0971 at 997.332 kb on - strand, within Dsui_0971 at 997.344 kb on - strand, within Dsui_0971 at 997.418 kb on + strand, within Dsui_0971 at 997.452 kb on - strand, within Dsui_0971 at 997.509 kb on - strand, within Dsui_0971 at 997.570 kb on - strand, within Dsui_0971 at 997.630 kb on + strand, within Dsui_0971 at 997.643 kb on - strand, within Dsui_0971 at 997.660 kb on - strand, within Dsui_0971
Per-strain Table
Position Strand Gene LocusTag Fraction spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 994,575 + Dsui_0969 0.34 -0.4 994,617 + Dsui_0969 0.38 -0.2 994,674 - Dsui_0969 0.43 -0.3 994,723 + Dsui_0969 0.47 -0.7 994,731 - Dsui_0969 0.48 -0.4 994,793 + Dsui_0969 0.53 +1.1 994,831 + Dsui_0969 0.56 +0.3 994,839 - Dsui_0969 0.57 -0.5 994,875 - Dsui_0969 0.60 -0.1 994,979 + Dsui_0969 0.69 +0.0 994,999 - Dsui_0969 0.71 -3.3 995,144 + Dsui_0969 0.83 +0.1 995,152 - +0.9 995,245 + +1.8 995,263 - -0.2 995,299 - +0.4 995,475 + -0.0 995,613 + Dsui_0970 0.11 +0.5 995,645 + Dsui_0970 0.13 +0.9 995,689 + Dsui_0970 0.16 +0.9 996,113 + Dsui_0970 0.49 +1.0 996,135 + Dsui_0970 0.51 +0.1 996,144 - Dsui_0970 0.52 -4.6 996,230 - -2.0 996,247 - Dsui_0970 0.60 -0.6 996,299 - Dsui_0970 0.64 +1.1 996,307 - Dsui_0970 0.64 -0.2 996,312 - Dsui_0970 0.65 +0.8 996,321 + Dsui_0970 0.65 +0.1 996,422 + Dsui_0970 0.73 +0.8 996,576 + Dsui_0970 0.85 +0.9 996,595 - Dsui_0970 0.87 +0.6 996,727 + +0.4 997,319 + Dsui_0971 0.26 -1.6 997,319 + Dsui_0971 0.26 +0.8 997,327 - Dsui_0971 0.27 -0.9 997,327 - Dsui_0971 0.27 -0.7 997,327 - Dsui_0971 0.27 -1.0 997,327 - Dsui_0971 0.27 -3.7 997,327 - Dsui_0971 0.27 -1.4 997,327 - Dsui_0971 0.27 -5.7 997,327 - Dsui_0971 0.27 -1.4 997,327 - Dsui_0971 0.27 -0.5 997,332 - Dsui_0971 0.27 -3.0 997,344 - Dsui_0971 0.28 -2.9 997,418 + Dsui_0971 0.32 -1.8 997,452 - Dsui_0971 0.33 -0.1 997,509 - Dsui_0971 0.37 -2.1 997,570 - Dsui_0971 0.40 -3.2 997,630 + Dsui_0971 0.43 -4.3 997,643 - Dsui_0971 0.44 -2.9 997,660 - Dsui_0971 0.45 -1.3
Or see this region's nucleotide sequence