Experiment: spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0684 and Dsui_0685 are separated by 30 nucleotides Dsui_0685 and Dsui_0686 are separated by 8 nucleotides Dsui_0686 and Dsui_0687 overlap by 17 nucleotides Dsui_0687 and Dsui_0688 are separated by 95 nucleotides Dsui_0688 and Dsui_0689 overlap by 1 nucleotides
Dsui_0684: Dsui_0684 - putative nucleoside-diphosphate sugar epimerase, at 682,383 to 683,342
_0684
Dsui_0685: Dsui_0685 - glutathione S-transferase, at 683,373 to 684,008
_0685
Dsui_0686: Dsui_0686 - tRNA nucleotidyltransferase/poly(A) polymerase, at 684,017 to 685,237
_0686
Dsui_0687: Dsui_0687 - Protein of unknown function (DUF2905), at 685,221 to 685,412
_0687
Dsui_0688: Dsui_0688 - glutathione S-transferase, at 685,508 to 686,125
_0688
Dsui_0689: Dsui_0689 - lysophospholipase, at 686,125 to 686,937
_0689
Position (kb) 684
685
686 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 683.019 kb on - strand, within Dsui_0684 at 683.019 kb on - strand, within Dsui_0684 at 683.023 kb on + strand, within Dsui_0684 at 683.031 kb on - strand, within Dsui_0684 at 683.031 kb on - strand, within Dsui_0684 at 683.043 kb on + strand, within Dsui_0684 at 683.046 kb on - strand, within Dsui_0684 at 683.047 kb on + strand, within Dsui_0684 at 683.135 kb on + strand, within Dsui_0684 at 683.278 kb on + strand at 683.492 kb on + strand, within Dsui_0685 at 683.520 kb on + strand, within Dsui_0685 at 683.800 kb on - strand at 683.830 kb on + strand, within Dsui_0685 at 683.899 kb on - strand, within Dsui_0685 at 685.268 kb on - strand, within Dsui_0687 at 685.274 kb on - strand, within Dsui_0687 at 685.318 kb on - strand, within Dsui_0687 at 685.379 kb on + strand, within Dsui_0687 at 685.387 kb on - strand, within Dsui_0687 at 685.422 kb on - strand at 685.493 kb on + strand at 685.501 kb on - strand at 685.513 kb on - strand at 685.514 kb on + strand at 685.525 kb on - strand at 685.574 kb on + strand, within Dsui_0688 at 685.579 kb on + strand, within Dsui_0688 at 685.579 kb on + strand, within Dsui_0688 at 685.579 kb on + strand, within Dsui_0688 at 685.579 kb on + strand, within Dsui_0688 at 685.587 kb on - strand at 685.587 kb on - strand, within Dsui_0688 at 685.587 kb on - strand, within Dsui_0688 at 685.587 kb on - strand, within Dsui_0688 at 685.587 kb on - strand, within Dsui_0688 at 685.587 kb on - strand at 685.616 kb on - strand at 685.616 kb on - strand, within Dsui_0688 at 685.641 kb on - strand, within Dsui_0688 at 685.701 kb on + strand, within Dsui_0688 at 685.776 kb on + strand, within Dsui_0688 at 685.877 kb on - strand, within Dsui_0688 at 685.921 kb on + strand, within Dsui_0688 at 685.921 kb on + strand, within Dsui_0688 at 685.940 kb on + strand, within Dsui_0688 at 685.952 kb on + strand, within Dsui_0688 at 685.952 kb on + strand, within Dsui_0688 at 686.001 kb on + strand, within Dsui_0688 at 686.106 kb on + strand at 686.114 kb on - strand at 686.114 kb on - strand at 686.190 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 683,019 - Dsui_0684 0.66 -1.2 683,019 - Dsui_0684 0.66 -3.7 683,023 + Dsui_0684 0.67 -1.1 683,031 - Dsui_0684 0.68 -3.5 683,031 - Dsui_0684 0.68 -3.4 683,043 + Dsui_0684 0.69 -0.8 683,046 - Dsui_0684 0.69 -2.6 683,047 + Dsui_0684 0.69 +0.5 683,135 + Dsui_0684 0.78 -1.0 683,278 + -0.1 683,492 + Dsui_0685 0.19 -3.5 683,520 + Dsui_0685 0.23 -2.8 683,800 - -2.3 683,830 + Dsui_0685 0.72 -3.5 683,899 - Dsui_0685 0.83 -0.6 685,268 - Dsui_0687 0.24 +0.8 685,274 - Dsui_0687 0.28 +0.5 685,318 - Dsui_0687 0.51 +0.1 685,379 + Dsui_0687 0.82 +0.3 685,387 - Dsui_0687 0.86 -0.4 685,422 - +1.4 685,493 + +0.2 685,501 - +0.2 685,513 - -0.3 685,514 + -0.5 685,525 - -0.1 685,574 + Dsui_0688 0.11 +0.2 685,579 + Dsui_0688 0.11 -2.3 685,579 + Dsui_0688 0.11 -0.3 685,579 + Dsui_0688 0.11 +0.6 685,579 + Dsui_0688 0.11 +0.4 685,587 - -2.3 685,587 - Dsui_0688 0.13 +0.2 685,587 - Dsui_0688 0.13 -2.3 685,587 - Dsui_0688 0.13 -0.2 685,587 - Dsui_0688 0.13 +0.1 685,587 - -3.2 685,616 - -0.5 685,616 - Dsui_0688 0.17 +0.6 685,641 - Dsui_0688 0.22 -0.4 685,701 + Dsui_0688 0.31 +0.0 685,776 + Dsui_0688 0.43 +0.6 685,877 - Dsui_0688 0.60 +0.2 685,921 + Dsui_0688 0.67 +0.2 685,921 + Dsui_0688 0.67 +0.6 685,940 + Dsui_0688 0.70 -0.6 685,952 + Dsui_0688 0.72 +0.1 685,952 + Dsui_0688 0.72 +1.3 686,001 + Dsui_0688 0.80 -0.1 686,106 + +0.3 686,114 - +0.4 686,114 - +0.2 686,190 + -3.9
Or see this region's nucleotide sequence