Experiment: spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0516 and Dsui_0517 are separated by 20 nucleotides Dsui_0517 and Dsui_0518 are separated by 47 nucleotides
Dsui_0516: Dsui_0516 - Propionyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.3) <small>(from data)</small>, at 520,438 to 522,429
_0516
Dsui_0517: Dsui_0517 - Propionyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.3) <small>(from data)</small>, at 522,450 to 523,982
_0517
Dsui_0518: Dsui_0518 - methylmalonyl-CoA-mutase-associated GTPase MeaB <small>(from data)</small>, at 524,030 to 525,058
_0518
Position (kb) 522
523
524 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 521.463 kb on - strand, within Dsui_0516 at 521.513 kb on - strand, within Dsui_0516 at 521.547 kb on + strand, within Dsui_0516 at 521.649 kb on - strand, within Dsui_0516 at 521.649 kb on - strand, within Dsui_0516 at 521.725 kb on - strand, within Dsui_0516 at 521.858 kb on - strand, within Dsui_0516 at 521.870 kb on + strand, within Dsui_0516 at 522.041 kb on + strand, within Dsui_0516 at 522.041 kb on + strand, within Dsui_0516 at 522.059 kb on + strand, within Dsui_0516 at 522.185 kb on + strand, within Dsui_0516 at 522.235 kb on - strand at 522.258 kb on + strand at 522.403 kb on + strand at 522.470 kb on - strand at 522.515 kb on + strand at 522.620 kb on - strand, within Dsui_0517 at 522.682 kb on + strand, within Dsui_0517 at 522.766 kb on - strand, within Dsui_0517 at 522.783 kb on - strand, within Dsui_0517 at 522.829 kb on + strand, within Dsui_0517 at 522.839 kb on + strand, within Dsui_0517 at 523.065 kb on - strand, within Dsui_0517 at 523.075 kb on - strand, within Dsui_0517 at 523.283 kb on - strand, within Dsui_0517 at 523.622 kb on - strand, within Dsui_0517 at 523.644 kb on + strand, within Dsui_0517 at 523.742 kb on - strand, within Dsui_0517 at 523.752 kb on + strand, within Dsui_0517 at 523.812 kb on - strand at 523.824 kb on - strand, within Dsui_0517 at 523.975 kb on + strand at 523.988 kb on - strand at 524.057 kb on - strand at 524.077 kb on - strand at 524.121 kb on + strand at 524.157 kb on + strand, within Dsui_0518 at 524.311 kb on - strand, within Dsui_0518 at 524.339 kb on + strand, within Dsui_0518 at 524.347 kb on - strand, within Dsui_0518 at 524.516 kb on - strand, within Dsui_0518 at 524.549 kb on - strand, within Dsui_0518 at 524.549 kb on - strand, within Dsui_0518 at 524.642 kb on - strand, within Dsui_0518 at 524.697 kb on + strand, within Dsui_0518 at 524.781 kb on - strand, within Dsui_0518 at 524.946 kb on + strand, within Dsui_0518
Per-strain Table
Position Strand Gene LocusTag Fraction spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 521,463 - Dsui_0516 0.51 -2.0 521,513 - Dsui_0516 0.54 -0.2 521,547 + Dsui_0516 0.56 +0.2 521,649 - Dsui_0516 0.61 +0.5 521,649 - Dsui_0516 0.61 -0.7 521,725 - Dsui_0516 0.65 -0.2 521,858 - Dsui_0516 0.71 +1.0 521,870 + Dsui_0516 0.72 -1.5 522,041 + Dsui_0516 0.80 -2.5 522,041 + Dsui_0516 0.80 -2.0 522,059 + Dsui_0516 0.81 -4.1 522,185 + Dsui_0516 0.88 -0.9 522,235 - -1.4 522,258 + -2.0 522,403 + +0.9 522,470 - -0.1 522,515 + -0.5 522,620 - Dsui_0517 0.11 -0.5 522,682 + Dsui_0517 0.15 -0.6 522,766 - Dsui_0517 0.21 -0.1 522,783 - Dsui_0517 0.22 -0.8 522,829 + Dsui_0517 0.25 -1.0 522,839 + Dsui_0517 0.25 -1.0 523,065 - Dsui_0517 0.40 +1.4 523,075 - Dsui_0517 0.41 -0.2 523,283 - Dsui_0517 0.54 -0.0 523,622 - Dsui_0517 0.76 +0.6 523,644 + Dsui_0517 0.78 -2.7 523,742 - Dsui_0517 0.84 -0.5 523,752 + Dsui_0517 0.85 +0.1 523,812 - -2.6 523,824 - Dsui_0517 0.90 +0.0 523,975 + +0.0 523,988 - -0.1 524,057 - +0.7 524,077 - +0.9 524,121 + -0.7 524,157 + Dsui_0518 0.12 -1.0 524,311 - Dsui_0518 0.27 -5.5 524,339 + Dsui_0518 0.30 -1.3 524,347 - Dsui_0518 0.31 +0.8 524,516 - Dsui_0518 0.47 -0.3 524,549 - Dsui_0518 0.50 +0.3 524,549 - Dsui_0518 0.50 +0.2 524,642 - Dsui_0518 0.59 +0.8 524,697 + Dsui_0518 0.65 +1.3 524,781 - Dsui_0518 0.73 -1.4 524,946 + Dsui_0518 0.89 -0.2
Or see this region's nucleotide sequence