Experiment: Recovery broth with Metronidazole 0.03 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14975 and BBR_RS14980 are separated by 147 nucleotides BBR_RS14980 and BBR_RS14985 are separated by 244 nucleotides BBR_RS14985 and BBR_RS14990 are separated by 120 nucleotides BBR_RS14990 and BBR_RS14995 are separated by 96 nucleotides
BBR_RS14975: BBR_RS14975 - purine permease, at 1,132,158 to 1,133,522
_RS14975
BBR_RS14980: BBR_RS14980 - xanthine phosphoribosyltransferase, at 1,133,670 to 1,134,251
_RS14980
BBR_RS14985: BBR_RS14985 - ATP-dependent DNA helicase PcrA, at 1,134,496 to 1,137,171
_RS14985
BBR_RS14990: BBR_RS14990 - DUF4418 domain-containing protein, at 1,137,292 to 1,137,687
_RS14990
BBR_RS14995: BBR_RS14995 - ABC transporter permease, at 1,137,784 to 1,139,001
_RS14995
Position (kb)
1134
1135
1136
1137
1138 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1133.544 kb on + strand at 1133.564 kb on - strand at 1133.587 kb on + strand at 1133.587 kb on - strand at 1133.648 kb on - strand at 1133.673 kb on - strand at 1133.675 kb on + strand at 1133.675 kb on + strand at 1133.675 kb on + strand at 1133.675 kb on + strand at 1133.680 kb on + strand at 1133.683 kb on - strand at 1133.683 kb on - strand at 1133.683 kb on - strand at 1133.794 kb on + strand, within BBR_RS14980 at 1133.832 kb on - strand, within BBR_RS14980 at 1133.832 kb on - strand, within BBR_RS14980 at 1133.928 kb on + strand, within BBR_RS14980 at 1133.953 kb on - strand, within BBR_RS14980 at 1133.953 kb on - strand, within BBR_RS14980 at 1133.955 kb on + strand, within BBR_RS14980 at 1133.955 kb on + strand, within BBR_RS14980 at 1133.990 kb on + strand, within BBR_RS14980 at 1134.029 kb on - strand, within BBR_RS14980 at 1134.182 kb on + strand, within BBR_RS14980 at 1134.213 kb on - strand at 1134.231 kb on - strand at 1134.256 kb on + strand at 1134.259 kb on + strand at 1134.314 kb on - strand at 1134.420 kb on - strand at 1134.420 kb on - strand at 1134.425 kb on - strand at 1134.425 kb on - strand at 1134.441 kb on + strand at 1136.572 kb on - strand, within BBR_RS14985 at 1136.755 kb on - strand, within BBR_RS14985 at 1136.757 kb on + strand, within BBR_RS14985 at 1136.757 kb on + strand, within BBR_RS14985 at 1136.788 kb on - strand, within BBR_RS14985 at 1136.838 kb on + strand, within BBR_RS14985 at 1136.846 kb on - strand, within BBR_RS14985 at 1137.095 kb on - strand at 1137.111 kb on - strand at 1137.119 kb on - strand at 1137.119 kb on - strand at 1137.221 kb on + strand at 1137.256 kb on + strand at 1137.289 kb on + strand at 1137.380 kb on - strand, within BBR_RS14990 at 1137.382 kb on + strand, within BBR_RS14990 at 1137.390 kb on + strand, within BBR_RS14990 at 1137.402 kb on + strand, within BBR_RS14990 at 1137.415 kb on + strand, within BBR_RS14990 at 1137.481 kb on + strand, within BBR_RS14990 at 1137.481 kb on + strand, within BBR_RS14990 at 1137.488 kb on - strand, within BBR_RS14990 at 1137.496 kb on + strand, within BBR_RS14990 at 1137.496 kb on + strand, within BBR_RS14990 at 1137.504 kb on - strand, within BBR_RS14990 at 1137.572 kb on - strand, within BBR_RS14990 at 1137.583 kb on - strand, within BBR_RS14990 at 1137.583 kb on - strand, within BBR_RS14990 at 1137.583 kb on - strand, within BBR_RS14990 at 1137.603 kb on - strand, within BBR_RS14990 at 1137.611 kb on - strand, within BBR_RS14990 at 1137.681 kb on + strand at 1137.686 kb on + strand at 1137.705 kb on - strand at 1137.848 kb on + strand at 1137.873 kb on - strand at 1137.899 kb on + strand at 1137.928 kb on + strand, within BBR_RS14995 at 1137.951 kb on + strand, within BBR_RS14995 at 1137.976 kb on + strand, within BBR_RS14995 at 1137.976 kb on + strand, within BBR_RS14995 at 1137.984 kb on - strand, within BBR_RS14995 at 1138.014 kb on - strand, within BBR_RS14995 at 1138.031 kb on - strand, within BBR_RS14995 at 1138.055 kb on + strand, within BBR_RS14995 at 1138.084 kb on + strand, within BBR_RS14995 at 1138.101 kb on + strand, within BBR_RS14995 at 1138.109 kb on - strand, within BBR_RS14995 at 1138.120 kb on - strand, within BBR_RS14995 at 1138.132 kb on - strand, within BBR_RS14995 at 1138.132 kb on - strand, within BBR_RS14995 at 1138.157 kb on + strand, within BBR_RS14995 at 1138.157 kb on + strand, within BBR_RS14995
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Metronidazole 0.03 mM remove 1,133,544 + +0.4 1,133,564 - -0.6 1,133,587 + -0.3 1,133,587 - -0.2 1,133,648 - +0.1 1,133,673 - +0.8 1,133,675 + -0.2 1,133,675 + +0.1 1,133,675 + -0.4 1,133,675 + -0.6 1,133,680 + +0.1 1,133,683 - -1.0 1,133,683 - -1.0 1,133,683 - -0.6 1,133,794 + BBR_RS14980 0.21 -1.2 1,133,832 - BBR_RS14980 0.28 +1.2 1,133,832 - BBR_RS14980 0.28 +0.9 1,133,928 + BBR_RS14980 0.44 +0.4 1,133,953 - BBR_RS14980 0.49 -1.3 1,133,953 - BBR_RS14980 0.49 +0.1 1,133,955 + BBR_RS14980 0.49 -0.7 1,133,955 + BBR_RS14980 0.49 -0.8 1,133,990 + BBR_RS14980 0.55 -1.2 1,134,029 - BBR_RS14980 0.62 +0.2 1,134,182 + BBR_RS14980 0.88 +0.4 1,134,213 - +0.1 1,134,231 - -0.6 1,134,256 + +1.3 1,134,259 + +0.6 1,134,314 - +0.5 1,134,420 - -0.0 1,134,420 - -1.2 1,134,425 - +0.6 1,134,425 - +0.2 1,134,441 + -1.0 1,136,572 - BBR_RS14985 0.78 +0.8 1,136,755 - BBR_RS14985 0.84 -1.6 1,136,757 + BBR_RS14985 0.84 -0.4 1,136,757 + BBR_RS14985 0.84 -1.8 1,136,788 - BBR_RS14985 0.86 -1.7 1,136,838 + BBR_RS14985 0.88 -0.9 1,136,846 - BBR_RS14985 0.88 -1.0 1,137,095 - -2.7 1,137,111 - +0.4 1,137,119 - +0.5 1,137,119 - -1.2 1,137,221 + -0.2 1,137,256 + +0.2 1,137,289 + +0.1 1,137,380 - BBR_RS14990 0.22 +0.1 1,137,382 + BBR_RS14990 0.23 -0.4 1,137,390 + BBR_RS14990 0.25 -0.6 1,137,402 + BBR_RS14990 0.28 -0.7 1,137,415 + BBR_RS14990 0.31 +0.7 1,137,481 + BBR_RS14990 0.48 -2.3 1,137,481 + BBR_RS14990 0.48 -0.7 1,137,488 - BBR_RS14990 0.49 +0.5 1,137,496 + BBR_RS14990 0.52 +0.0 1,137,496 + BBR_RS14990 0.52 -0.0 1,137,504 - BBR_RS14990 0.54 -0.5 1,137,572 - BBR_RS14990 0.71 +0.4 1,137,583 - BBR_RS14990 0.73 -0.3 1,137,583 - BBR_RS14990 0.73 -0.1 1,137,583 - BBR_RS14990 0.73 -0.7 1,137,603 - BBR_RS14990 0.79 +2.3 1,137,611 - BBR_RS14990 0.81 -0.2 1,137,681 + -0.8 1,137,686 + -0.2 1,137,705 - -0.9 1,137,848 + -0.9 1,137,873 - +1.1 1,137,899 + +0.3 1,137,928 + BBR_RS14995 0.12 +0.2 1,137,951 + BBR_RS14995 0.14 +0.8 1,137,976 + BBR_RS14995 0.16 -0.4 1,137,976 + BBR_RS14995 0.16 +0.2 1,137,984 - BBR_RS14995 0.16 -0.8 1,138,014 - BBR_RS14995 0.19 +0.4 1,138,031 - BBR_RS14995 0.20 +0.5 1,138,055 + BBR_RS14995 0.22 +2.9 1,138,084 + BBR_RS14995 0.25 -0.8 1,138,101 + BBR_RS14995 0.26 +0.7 1,138,109 - BBR_RS14995 0.27 +2.3 1,138,120 - BBR_RS14995 0.28 -0.1 1,138,132 - BBR_RS14995 0.29 +0.1 1,138,132 - BBR_RS14995 0.29 -0.1 1,138,157 + BBR_RS14995 0.31 +1.8 1,138,157 + BBR_RS14995 0.31 -1.0
Or see this region's nucleotide sequence