Experiment: Recovery broth with Metronidazole 0.03 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS10550 and BBR_RS10555 are separated by 133 nucleotides BBR_RS10555 and BBR_RS10560 are separated by 212 nucleotides
BBR_RS10550: BBR_RS10550 - tryptophan--tRNA ligase, at 84,703 to 85,794
_RS10550
BBR_RS10555: BBR_RS10555 - DUF3073 domain-containing protein, at 85,928 to 86,311
_RS10555
BBR_RS10560: BBR_RS10560 - hypothetical protein, at 86,524 to 87,093
_RS10560
Position (kb) 85
86
87 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 85.777 kb on - strand at 85.810 kb on - strand at 85.810 kb on - strand at 85.867 kb on + strand at 85.873 kb on + strand at 85.875 kb on - strand at 85.875 kb on - strand at 85.879 kb on + strand at 85.881 kb on - strand at 85.904 kb on + strand at 85.920 kb on + strand at 86.052 kb on + strand, within BBR_RS10555 at 86.235 kb on + strand, within BBR_RS10555 at 86.275 kb on + strand at 86.275 kb on + strand at 86.283 kb on - strand at 86.296 kb on + strand at 86.296 kb on + strand at 86.296 kb on + strand at 86.315 kb on - strand at 86.315 kb on - strand at 86.317 kb on + strand at 86.325 kb on - strand at 86.351 kb on - strand at 86.352 kb on + strand at 86.361 kb on + strand at 86.361 kb on + strand at 86.361 kb on - strand at 86.369 kb on - strand at 86.373 kb on + strand at 86.401 kb on + strand at 86.401 kb on + strand at 86.401 kb on + strand at 86.425 kb on - strand at 86.425 kb on - strand at 86.440 kb on - strand at 86.447 kb on + strand at 86.475 kb on + strand at 86.493 kb on + strand at 86.520 kb on - strand at 86.530 kb on - strand at 86.535 kb on - strand at 86.536 kb on - strand at 86.559 kb on + strand at 86.567 kb on + strand at 86.572 kb on + strand at 86.575 kb on - strand at 86.580 kb on - strand at 86.580 kb on - strand at 86.586 kb on + strand, within BBR_RS10560 at 86.598 kb on + strand, within BBR_RS10560 at 86.598 kb on + strand, within BBR_RS10560 at 86.600 kb on + strand, within BBR_RS10560 at 86.606 kb on - strand, within BBR_RS10560 at 86.608 kb on - strand, within BBR_RS10560 at 86.612 kb on + strand, within BBR_RS10560 at 86.612 kb on + strand, within BBR_RS10560 at 86.617 kb on + strand, within BBR_RS10560 at 86.619 kb on - strand, within BBR_RS10560 at 86.655 kb on + strand, within BBR_RS10560 at 86.655 kb on + strand, within BBR_RS10560 at 86.663 kb on - strand, within BBR_RS10560 at 86.663 kb on - strand, within BBR_RS10560 at 86.663 kb on - strand, within BBR_RS10560 at 86.664 kb on + strand, within BBR_RS10560 at 86.664 kb on - strand, within BBR_RS10560 at 86.665 kb on + strand, within BBR_RS10560 at 86.665 kb on + strand, within BBR_RS10560 at 86.669 kb on + strand, within BBR_RS10560 at 86.670 kb on + strand, within BBR_RS10560 at 86.672 kb on - strand, within BBR_RS10560 at 86.673 kb on - strand, within BBR_RS10560 at 86.678 kb on - strand, within BBR_RS10560 at 86.678 kb on - strand, within BBR_RS10560 at 86.685 kb on + strand, within BBR_RS10560 at 86.687 kb on - strand, within BBR_RS10560 at 86.719 kb on - strand, within BBR_RS10560 at 86.724 kb on - strand, within BBR_RS10560 at 86.728 kb on + strand, within BBR_RS10560 at 86.728 kb on + strand, within BBR_RS10560 at 86.733 kb on + strand, within BBR_RS10560 at 86.733 kb on + strand, within BBR_RS10560 at 86.734 kb on - strand, within BBR_RS10560 at 86.741 kb on - strand, within BBR_RS10560 at 86.747 kb on - strand, within BBR_RS10560 at 86.760 kb on + strand, within BBR_RS10560 at 86.760 kb on + strand, within BBR_RS10560 at 86.785 kb on - strand, within BBR_RS10560 at 86.785 kb on - strand, within BBR_RS10560 at 86.789 kb on + strand, within BBR_RS10560 at 86.794 kb on + strand, within BBR_RS10560 at 86.796 kb on + strand, within BBR_RS10560 at 86.797 kb on - strand, within BBR_RS10560 at 86.798 kb on - strand, within BBR_RS10560 at 86.809 kb on + strand, within BBR_RS10560 at 86.814 kb on + strand, within BBR_RS10560 at 86.816 kb on + strand, within BBR_RS10560 at 86.816 kb on + strand, within BBR_RS10560 at 86.817 kb on - strand, within BBR_RS10560 at 86.822 kb on - strand, within BBR_RS10560 at 86.824 kb on - strand, within BBR_RS10560 at 86.824 kb on - strand, within BBR_RS10560 at 86.827 kb on + strand, within BBR_RS10560 at 86.835 kb on - strand, within BBR_RS10560 at 86.835 kb on - strand, within BBR_RS10560 at 86.844 kb on - strand, within BBR_RS10560 at 86.852 kb on - strand, within BBR_RS10560 at 86.853 kb on + strand, within BBR_RS10560 at 86.861 kb on - strand, within BBR_RS10560 at 86.880 kb on + strand, within BBR_RS10560 at 86.880 kb on + strand, within BBR_RS10560 at 86.884 kb on - strand, within BBR_RS10560 at 86.905 kb on - strand, within BBR_RS10560 at 86.912 kb on + strand, within BBR_RS10560 at 86.938 kb on + strand, within BBR_RS10560 at 86.946 kb on - strand, within BBR_RS10560 at 86.948 kb on + strand, within BBR_RS10560 at 86.960 kb on + strand, within BBR_RS10560 at 86.978 kb on + strand, within BBR_RS10560 at 87.045 kb on - strand at 87.045 kb on - strand at 87.096 kb on + strand at 87.096 kb on + strand at 87.096 kb on + strand at 87.102 kb on + strand at 87.110 kb on - strand at 87.115 kb on - strand at 87.180 kb on - strand at 87.221 kb on + strand at 87.221 kb on + strand at 87.221 kb on + strand at 87.223 kb on + strand at 87.223 kb on + strand at 87.229 kb on - strand at 87.229 kb on - strand at 87.229 kb on - strand at 87.231 kb on - strand at 87.231 kb on - strand at 87.231 kb on - strand at 87.231 kb on - strand at 87.231 kb on - strand at 87.231 kb on - strand at 87.241 kb on - strand at 87.244 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Metronidazole 0.03 mM remove 85,777 - -0.6 85,810 - +0.2 85,810 - +0.1 85,867 + +1.4 85,873 + +3.2 85,875 - +1.1 85,875 - +1.7 85,879 + +2.1 85,881 - +2.7 85,904 + +3.6 85,920 + +2.6 86,052 + BBR_RS10555 0.32 +3.8 86,235 + BBR_RS10555 0.80 +3.0 86,275 + +1.6 86,275 + +3.1 86,283 - +3.7 86,296 + +3.7 86,296 + +2.9 86,296 + +3.4 86,315 - +0.9 86,315 - +0.3 86,317 + -0.0 86,325 - +0.5 86,351 - +0.6 86,352 + +0.5 86,361 + +0.6 86,361 + +0.4 86,361 - +0.1 86,369 - +0.2 86,373 + -0.2 86,401 + -1.2 86,401 + -0.7 86,401 + +0.4 86,425 - +0.7 86,425 - +1.4 86,440 - -0.2 86,447 + +0.5 86,475 + +0.8 86,493 + -0.5 86,520 - +0.4 86,530 - +0.4 86,535 - +0.7 86,536 - +0.5 86,559 + -3.7 86,567 + -1.2 86,572 + -2.7 86,575 - -2.1 86,580 - -2.4 86,580 - -2.3 86,586 + BBR_RS10560 0.11 -3.2 86,598 + BBR_RS10560 0.13 -3.3 86,598 + BBR_RS10560 0.13 -1.9 86,600 + BBR_RS10560 0.13 -2.5 86,606 - BBR_RS10560 0.14 -2.8 86,608 - BBR_RS10560 0.15 -3.3 86,612 + BBR_RS10560 0.15 -2.1 86,612 + BBR_RS10560 0.15 -2.8 86,617 + BBR_RS10560 0.16 -1.7 86,619 - BBR_RS10560 0.17 -3.0 86,655 + BBR_RS10560 0.23 -3.1 86,655 + BBR_RS10560 0.23 -3.0 86,663 - BBR_RS10560 0.24 -2.6 86,663 - BBR_RS10560 0.24 -2.7 86,663 - BBR_RS10560 0.24 -2.7 86,664 + BBR_RS10560 0.25 -0.6 86,664 - BBR_RS10560 0.25 -2.7 86,665 + BBR_RS10560 0.25 -3.9 86,665 + BBR_RS10560 0.25 -2.3 86,669 + BBR_RS10560 0.25 -2.5 86,670 + BBR_RS10560 0.26 -0.8 86,672 - BBR_RS10560 0.26 -3.9 86,673 - BBR_RS10560 0.26 -3.1 86,678 - BBR_RS10560 0.27 -2.1 86,678 - BBR_RS10560 0.27 -2.8 86,685 + BBR_RS10560 0.28 -1.4 86,687 - BBR_RS10560 0.29 -2.2 86,719 - BBR_RS10560 0.34 -3.6 86,724 - BBR_RS10560 0.35 -2.0 86,728 + BBR_RS10560 0.36 -2.6 86,728 + BBR_RS10560 0.36 -3.1 86,733 + BBR_RS10560 0.37 -3.5 86,733 + BBR_RS10560 0.37 -1.9 86,734 - BBR_RS10560 0.37 -2.3 86,741 - BBR_RS10560 0.38 -2.0 86,747 - BBR_RS10560 0.39 -3.1 86,760 + BBR_RS10560 0.41 -2.5 86,760 + BBR_RS10560 0.41 -2.3 86,785 - BBR_RS10560 0.46 -3.7 86,785 - BBR_RS10560 0.46 -4.1 86,789 + BBR_RS10560 0.46 -2.7 86,794 + BBR_RS10560 0.47 -3.0 86,796 + BBR_RS10560 0.48 +0.1 86,797 - BBR_RS10560 0.48 -1.3 86,798 - BBR_RS10560 0.48 -3.6 86,809 + BBR_RS10560 0.50 -0.8 86,814 + BBR_RS10560 0.51 -2.3 86,816 + BBR_RS10560 0.51 -3.0 86,816 + BBR_RS10560 0.51 -3.5 86,817 - BBR_RS10560 0.51 -3.3 86,822 - BBR_RS10560 0.52 -3.6 86,824 - BBR_RS10560 0.53 -2.3 86,824 - BBR_RS10560 0.53 -2.1 86,827 + BBR_RS10560 0.53 -3.1 86,835 - BBR_RS10560 0.55 -0.7 86,835 - BBR_RS10560 0.55 -2.5 86,844 - BBR_RS10560 0.56 -3.4 86,852 - BBR_RS10560 0.58 -3.0 86,853 + BBR_RS10560 0.58 -2.5 86,861 - BBR_RS10560 0.59 -3.6 86,880 + BBR_RS10560 0.62 +0.1 86,880 + BBR_RS10560 0.62 -0.4 86,884 - BBR_RS10560 0.63 -3.7 86,905 - BBR_RS10560 0.67 -2.4 86,912 + BBR_RS10560 0.68 -1.8 86,938 + BBR_RS10560 0.73 -4.1 86,946 - BBR_RS10560 0.74 -3.3 86,948 + BBR_RS10560 0.74 -3.1 86,960 + BBR_RS10560 0.76 -3.2 86,978 + BBR_RS10560 0.80 -3.8 87,045 - -2.6 87,045 - -3.5 87,096 + +0.0 87,096 + -1.2 87,096 + -0.3 87,102 + +0.7 87,110 - +1.0 87,115 - +1.2 87,180 - +0.4 87,221 + -0.2 87,221 + -0.5 87,221 + +0.0 87,223 + +0.1 87,223 + +0.6 87,229 - +0.0 87,229 - -0.2 87,229 - +0.0 87,231 - -0.3 87,231 - +0.2 87,231 - -1.4 87,231 - -0.0 87,231 - -0.2 87,231 - +0.7 87,241 - -0.7 87,244 + -0.6
Or see this region's nucleotide sequence