Experiment: Cholin acetate 30 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3212 and Shewana3_3213 are separated by 365 nucleotides Shewana3_3213 and Shewana3_3214 are separated by 202 nucleotides Shewana3_3214 and Shewana3_3215 are separated by 22 nucleotides
Shewana3_3212: Shewana3_3212 - methyl-accepting chemotaxis sensory transducer (RefSeq), at 3,855,835 to 3,857,856
_3212
Shewana3_3213: Shewana3_3213 - hypothetical protein (RefSeq), at 3,858,222 to 3,858,635
_3213
Shewana3_3214: Shewana3_3214 - dihydrofolate reductase (RefSeq), at 3,858,838 to 3,859,320
_3214
Shewana3_3215: Shewana3_3215 - hypothetical protein (RefSeq), at 3,859,343 to 3,859,816
_3215
Position (kb)
3858
3859 Strain fitness (log2 ratio)
-1
0
1
2 at 3857.308 kb on + strand, within Shewana3_3212 at 3857.486 kb on - strand, within Shewana3_3212 at 3857.527 kb on + strand, within Shewana3_3212 at 3857.591 kb on - strand, within Shewana3_3212 at 3858.065 kb on + strand at 3858.281 kb on + strand, within Shewana3_3213 at 3858.307 kb on + strand, within Shewana3_3213 at 3858.418 kb on - strand, within Shewana3_3213 at 3858.821 kb on + strand at 3859.467 kb on - strand, within Shewana3_3215 at 3859.570 kb on - strand, within Shewana3_3215
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 30 mM remove 3,857,308 + Shewana3_3212 0.73 -0.9 3,857,486 - Shewana3_3212 0.82 -0.3 3,857,527 + Shewana3_3212 0.84 +0.0 3,857,591 - Shewana3_3212 0.87 -0.3 3,858,065 + +0.1 3,858,281 + Shewana3_3213 0.14 +1.0 3,858,307 + Shewana3_3213 0.21 +1.0 3,858,418 - Shewana3_3213 0.47 -1.0 3,858,821 + +1.0 3,859,467 - Shewana3_3215 0.26 +1.8 3,859,570 - Shewana3_3215 0.48 -1.7
Or see this region's nucleotide sequence