Experiment: Cholin acetate 30 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2524 and grxA are separated by 60 nucleotides grxA and Shewana3_2526 are separated by 58 nucleotides Shewana3_2526 and Shewana3_R0104 are separated by 167 nucleotides Shewana3_R0104 and Shewana3_R0105 are separated by 160 nucleotides Shewana3_R0105 and Shewana3_R0106 are separated by 160 nucleotides Shewana3_R0106 and Shewana3_R0107 are separated by 35 nucleotides Shewana3_R0107 and Shewana3_R0108 are separated by 36 nucleotides Shewana3_R0108 and Shewana3_R0109 are separated by 35 nucleotides
Shewana3_2524: Shewana3_2524 - type III restriction enzyme, res subunit (RefSeq), at 3,021,537 to 3,023,309
_2524
Shewana3_2525: grxA - glutaredoxin 1 (RefSeq), at 3,023,370 to 3,023,630
grxA
Shewana3_2526: Shewana3_2526 - hypothetical protein (RefSeq), at 3,023,689 to 3,024,852
_2526
Shewana3_R0104: Shewana3_R0104 - tRNA-Lys (RefSeq), at 3,025,020 to 3,025,095
_R0104
Shewana3_R0105: Shewana3_R0105 - tRNA-Lys (RefSeq), at 3,025,256 to 3,025,331
_R0105
Shewana3_R0106: Shewana3_R0106 - tRNA-Lys (RefSeq), at 3,025,492 to 3,025,567
_R0106
Shewana3_R0107: Shewana3_R0107 - tRNA-Lys (RefSeq), at 3,025,603 to 3,025,678
_R0107
Shewana3_R0108: Shewana3_R0108 - tRNA-Lys (RefSeq), at 3,025,715 to 3,025,790
_R0108
Shewana3_R0109: Shewana3_R0109 - tRNA-Lys (RefSeq), at 3,025,826 to 3,025,901
_R0109
Position (kb)
3023
3024
3025 Strain fitness (log2 ratio)
-1
0
1 at 3022.864 kb on - strand, within Shewana3_2524 at 3022.878 kb on + strand, within Shewana3_2524 at 3023.198 kb on - strand at 3023.335 kb on - strand at 3023.755 kb on + strand at 3023.775 kb on - strand at 3023.855 kb on - strand, within Shewana3_2526 at 3023.893 kb on + strand, within Shewana3_2526 at 3023.895 kb on + strand, within Shewana3_2526 at 3024.256 kb on + strand, within Shewana3_2526 at 3024.690 kb on - strand, within Shewana3_2526
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 30 mM remove 3,022,864 - Shewana3_2524 0.75 +0.9 3,022,878 + Shewana3_2524 0.76 -0.1 3,023,198 - +0.9 3,023,335 - +0.9 3,023,755 + +0.9 3,023,775 - +1.5 3,023,855 - Shewana3_2526 0.14 -1.2 3,023,893 + Shewana3_2526 0.18 +0.9 3,023,895 + Shewana3_2526 0.18 -0.1 3,024,256 + Shewana3_2526 0.49 +0.9 3,024,690 - Shewana3_2526 0.86 +0.9
Or see this region's nucleotide sequence