Experiment: Cholin acetate 30 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2470 and Shewana3_2471 are separated by 62 nucleotides Shewana3_2471 and Shewana3_2472 overlap by 1 nucleotides Shewana3_2472 and Shewana3_2473 are separated by 106 nucleotides
Shewana3_2470: Shewana3_2470 - hypothetical protein (RefSeq), at 2,957,393 to 2,957,800
_2470
Shewana3_2471: Shewana3_2471 - alpha/beta fold family hydrolase (RefSeq), at 2,957,863 to 2,958,732
_2471
Shewana3_2472: Shewana3_2472 - oxoglutarate dehydrogenase (succinyl-transferring) (RefSeq), at 2,958,732 to 2,959,757
_2472
Shewana3_2473: Shewana3_2473 - succinate dehydrogenase (ubiquinone) (RefSeq), at 2,959,864 to 2,960,595
_2473
Position (kb)
2958
2959
2960 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2957.779 kb on + strand at 2957.868 kb on - strand at 2957.918 kb on - strand at 2957.994 kb on - strand, within Shewana3_2471 at 2958.142 kb on - strand, within Shewana3_2471 at 2958.332 kb on + strand, within Shewana3_2471 at 2958.665 kb on - strand at 2958.843 kb on + strand, within Shewana3_2472 at 2959.183 kb on - strand, within Shewana3_2472 at 2959.226 kb on + strand, within Shewana3_2472 at 2959.459 kb on - strand, within Shewana3_2472 at 2959.840 kb on + strand at 2960.210 kb on + strand, within Shewana3_2473 at 2960.250 kb on - strand, within Shewana3_2473 at 2960.611 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 30 mM remove 2,957,779 + -0.5 2,957,868 - +1.9 2,957,918 - +0.4 2,957,994 - Shewana3_2471 0.15 +0.3 2,958,142 - Shewana3_2471 0.32 +0.3 2,958,332 + Shewana3_2471 0.54 +0.6 2,958,665 - -0.1 2,958,843 + Shewana3_2472 0.11 +0.9 2,959,183 - Shewana3_2472 0.44 +0.9 2,959,226 + Shewana3_2472 0.48 +0.9 2,959,459 - Shewana3_2472 0.71 -2.1 2,959,840 + -0.7 2,960,210 + Shewana3_2473 0.47 +0.9 2,960,250 - Shewana3_2473 0.53 +1.9 2,960,611 + +1.3
Or see this region's nucleotide sequence