Experiment: Cholin acetate 30 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0705 and Shewana3_0706 overlap by 17 nucleotides Shewana3_0706 and Shewana3_0707 overlap by 1 nucleotides Shewana3_0707 and thiH are separated by 0 nucleotides
Shewana3_0705: Shewana3_0705 - small GTP-binding protein (RefSeq), at 819,881 to 821,119
_0705
Shewana3_0706: Shewana3_0706 - biotin synthase (RefSeq), at 821,103 to 822,182
_0706
Shewana3_0707: Shewana3_0707 - hypothetical protein (RefSeq), at 822,182 to 822,820
_0707
Shewana3_0708: thiH - thiamine biosynthesis protein ThiH (RefSeq), at 822,821 to 824,260
thiH
Position (kb) 821
822
823 Strain fitness (log2 ratio) -2
-1
0
1
2 at 820.271 kb on - strand, within Shewana3_0705 at 820.655 kb on + strand, within Shewana3_0705 at 820.686 kb on + strand, within Shewana3_0705 at 820.700 kb on + strand, within Shewana3_0705 at 820.785 kb on - strand, within Shewana3_0705 at 820.880 kb on + strand, within Shewana3_0705 at 821.011 kb on + strand at 821.109 kb on + strand at 821.144 kb on + strand at 821.144 kb on + strand at 821.144 kb on + strand at 821.170 kb on - strand at 821.402 kb on + strand, within Shewana3_0706 at 821.402 kb on + strand, within Shewana3_0706 at 821.584 kb on - strand, within Shewana3_0706 at 821.841 kb on + strand, within Shewana3_0706 at 821.854 kb on - strand, within Shewana3_0706 at 821.877 kb on - strand, within Shewana3_0706 at 822.083 kb on + strand at 822.102 kb on + strand at 822.268 kb on + strand, within Shewana3_0707 at 822.268 kb on + strand, within Shewana3_0707 at 822.352 kb on - strand, within Shewana3_0707 at 822.352 kb on - strand, within Shewana3_0707 at 822.365 kb on - strand, within Shewana3_0707 at 822.572 kb on - strand, within Shewana3_0707 at 822.618 kb on + strand, within Shewana3_0707 at 822.630 kb on - strand, within Shewana3_0707 at 822.874 kb on + strand at 822.882 kb on - strand at 822.926 kb on - strand at 822.981 kb on - strand, within thiH at 822.981 kb on - strand, within thiH at 823.154 kb on + strand, within thiH
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 30 mM remove 820,271 - Shewana3_0705 0.31 -0.3 820,655 + Shewana3_0705 0.62 -0.4 820,686 + Shewana3_0705 0.65 +0.4 820,700 + Shewana3_0705 0.66 -0.2 820,785 - Shewana3_0705 0.73 +1.0 820,880 + Shewana3_0705 0.81 +0.4 821,011 + +0.7 821,109 + -0.4 821,144 + -0.1 821,144 + -0.4 821,144 + +0.1 821,170 - +0.8 821,402 + Shewana3_0706 0.28 -0.4 821,402 + Shewana3_0706 0.28 -0.2 821,584 - Shewana3_0706 0.45 -1.0 821,841 + Shewana3_0706 0.68 -1.0 821,854 - Shewana3_0706 0.70 -1.2 821,877 - Shewana3_0706 0.72 -2.0 822,083 + -0.9 822,102 + +0.7 822,268 + Shewana3_0707 0.13 +0.3 822,268 + Shewana3_0707 0.13 +0.4 822,352 - Shewana3_0707 0.27 +0.2 822,352 - Shewana3_0707 0.27 +1.0 822,365 - Shewana3_0707 0.29 -1.7 822,572 - Shewana3_0707 0.61 -0.6 822,618 + Shewana3_0707 0.68 +1.0 822,630 - Shewana3_0707 0.70 -1.0 822,874 + +2.6 822,882 - +1.6 822,926 - -0.3 822,981 - thiH Shewana3_0708 0.11 +0.4 822,981 - thiH Shewana3_0708 0.11 +0.3 823,154 + thiH Shewana3_0708 0.23 +0.1
Or see this region's nucleotide sequence