Experiment: MoYLS4 with Antimycin A 0.2uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVUA0116 and DORF2111 are separated by 42 nucleotides DORF2111 and escJ are separated by 24 nucleotides escJ and DORF2116 are separated by 6 nucleotides DORF2116 and DVUA0118 overlap by 22 nucleotides
DVUA0116: DVUA0116 - conserved hypothetical protein (TIGR), at 153,722 to 154,087
DVUA0116
DORF2111: DORF2111 - SEED: FIG00602408: hypothetical protein, at 154,130 to 154,945
DORF2111
DVUA0117: escJ - type III secretion lipoprotein (TIGR), at 154,970 to 155,785
escJ
DORF2116: DORF2116 - SEED: FIG00606188: hypothetical protein, at 155,792 to 156,715
DORF2116
DVUA0118: DVUA0118 - type III secretion protein, YopL family (TIGR), at 156,694 to 157,311
DVUA0118
Position (kb) 154
155
156 Strain fitness (log2 ratio) -2
-1
0
1 at 153.973 kb on + strand, within DVUA0116 at 153.974 kb on + strand, within DVUA0116 at 154.003 kb on + strand, within DVUA0116 at 154.027 kb on + strand, within DVUA0116 at 154.085 kb on - strand at 154.167 kb on + strand at 154.210 kb on + strand at 154.245 kb on + strand, within DORF2111 at 154.282 kb on + strand, within DORF2111 at 154.344 kb on + strand, within DORF2111 at 154.362 kb on - strand, within DORF2111 at 154.445 kb on + strand, within DORF2111 at 154.452 kb on + strand, within DORF2111 at 154.452 kb on + strand, within DORF2111 at 154.465 kb on + strand, within DORF2111 at 154.500 kb on + strand, within DORF2111 at 154.534 kb on + strand, within DORF2111 at 154.542 kb on - strand, within DORF2111 at 154.542 kb on - strand, within DORF2111 at 154.589 kb on + strand, within DORF2111 at 154.656 kb on - strand, within DORF2111 at 154.665 kb on + strand, within DORF2111 at 154.699 kb on - strand, within DORF2111 at 154.722 kb on - strand, within DORF2111 at 154.834 kb on + strand, within DORF2111 at 154.841 kb on + strand, within DORF2111 at 154.849 kb on - strand, within DORF2111 at 154.886 kb on + strand at 154.892 kb on - strand at 154.904 kb on + strand at 154.976 kb on - strand at 154.997 kb on + strand at 155.041 kb on - strand at 155.095 kb on + strand, within escJ at 155.103 kb on - strand, within escJ at 155.103 kb on - strand, within escJ at 155.157 kb on - strand, within escJ at 155.166 kb on + strand, within escJ at 155.174 kb on - strand, within escJ at 155.190 kb on + strand, within escJ at 155.268 kb on + strand, within escJ at 155.445 kb on + strand, within escJ at 155.459 kb on - strand, within escJ at 155.512 kb on + strand, within escJ at 155.687 kb on + strand, within escJ at 155.765 kb on + strand at 155.775 kb on + strand at 155.780 kb on + strand at 155.839 kb on + strand at 155.839 kb on + strand at 155.847 kb on - strand at 156.146 kb on + strand, within DORF2116 at 156.160 kb on + strand, within DORF2116 at 156.206 kb on - strand, within DORF2116 at 156.231 kb on + strand, within DORF2116 at 156.231 kb on + strand, within DORF2116 at 156.235 kb on + strand, within DORF2116 at 156.236 kb on + strand, within DORF2116 at 156.254 kb on + strand, within DORF2116 at 156.325 kb on - strand, within DORF2116 at 156.339 kb on - strand, within DORF2116 at 156.344 kb on + strand, within DORF2116 at 156.419 kb on + strand, within DORF2116 at 156.419 kb on + strand, within DORF2116 at 156.574 kb on + strand, within DORF2116 at 156.608 kb on - strand, within DORF2116 at 156.713 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Antimycin A 0.2uM remove 153,973 + DVUA0116 0.69 -0.6 153,974 + DVUA0116 0.69 -0.4 154,003 + DVUA0116 0.77 +0.7 154,027 + DVUA0116 0.83 -0.1 154,085 - -1.2 154,167 + -2.1 154,210 + -0.4 154,245 + DORF2111 0.14 +0.8 154,282 + DORF2111 0.19 -0.7 154,344 + DORF2111 0.26 +0.1 154,362 - DORF2111 0.28 -0.5 154,445 + DORF2111 0.39 +0.2 154,452 + DORF2111 0.39 +0.0 154,452 + DORF2111 0.39 -0.5 154,465 + DORF2111 0.41 -1.6 154,500 + DORF2111 0.45 -0.2 154,534 + DORF2111 0.50 -2.3 154,542 - DORF2111 0.50 -0.0 154,542 - DORF2111 0.50 +0.5 154,589 + DORF2111 0.56 -1.0 154,656 - DORF2111 0.64 -0.1 154,665 + DORF2111 0.66 -0.4 154,699 - DORF2111 0.70 -0.5 154,722 - DORF2111 0.73 +0.9 154,834 + DORF2111 0.86 +0.1 154,841 + DORF2111 0.87 -1.2 154,849 - DORF2111 0.88 -0.5 154,886 + -2.5 154,892 - +0.3 154,904 + -0.8 154,976 - -0.0 154,997 + +1.2 155,041 - +0.4 155,095 + escJ DVUA0117 0.15 -0.8 155,103 - escJ DVUA0117 0.16 -1.9 155,103 - escJ DVUA0117 0.16 -1.1 155,157 - escJ DVUA0117 0.23 -0.4 155,166 + escJ DVUA0117 0.24 -0.4 155,174 - escJ DVUA0117 0.25 -1.3 155,190 + escJ DVUA0117 0.27 -1.0 155,268 + escJ DVUA0117 0.37 -0.7 155,445 + escJ DVUA0117 0.58 -0.6 155,459 - escJ DVUA0117 0.60 -0.8 155,512 + escJ DVUA0117 0.66 -0.3 155,687 + escJ DVUA0117 0.88 -0.4 155,765 + +0.1 155,775 + -1.7 155,780 + +0.3 155,839 + -0.9 155,839 + +0.1 155,847 - -0.6 156,146 + DORF2116 0.38 -0.8 156,160 + DORF2116 0.40 +0.0 156,206 - DORF2116 0.45 +0.0 156,231 + DORF2116 0.48 +0.7 156,231 + DORF2116 0.48 +0.5 156,235 + DORF2116 0.48 +0.2 156,236 + DORF2116 0.48 +0.2 156,254 + DORF2116 0.50 -0.1 156,325 - DORF2116 0.58 +0.1 156,339 - DORF2116 0.59 -0.1 156,344 + DORF2116 0.60 -0.1 156,419 + DORF2116 0.68 +0.4 156,419 + DORF2116 0.68 +0.7 156,574 + DORF2116 0.85 -0.3 156,608 - DORF2116 0.88 -0.1 156,713 - +0.7
Or see this region's nucleotide sequence