Experiment: Formate deepwell autotrophic conditions
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05080 and MMP_RS05085 are separated by 14 nucleotides MMP_RS05085 and MMP_RS05090 are separated by 10 nucleotides MMP_RS05090 and MMP_RS05095 are separated by 11 nucleotides MMP_RS05095 and MMP_RS05100 are separated by 9 nucleotides
MMP_RS05080: MMP_RS05080 - acetyl-CoA decarbonylase/synthase complex subunit delta, at 966,196 to 967,350
_RS05080
MMP_RS05085: MMP_RS05085 - AAA family ATPase, at 967,365 to 968,117
_RS05085
MMP_RS05090: MMP_RS05090 - CO dehydrogenase/CO-methylating acetyl-CoA synthase complex subunit beta, at 968,128 to 969,501
_RS05090
MMP_RS05095: MMP_RS05095 - CO dehydrogenase/acetyl-CoA synthase complex subunit epsilon, at 969,513 to 970,031
_RS05095
MMP_RS05100: MMP_RS05100 - CO dehydrogenase/acetyl-CoA synthase complex subunit alpha, at 970,041 to 972,383
_RS05100
Position (kb) 968
969
970 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 967.467 kb on + strand, within MMP_RS05085 at 967.483 kb on + strand, within MMP_RS05085 at 967.570 kb on - strand, within MMP_RS05085 at 967.732 kb on + strand, within MMP_RS05085 at 967.741 kb on - strand, within MMP_RS05085 at 967.742 kb on + strand, within MMP_RS05085 at 967.781 kb on - strand, within MMP_RS05085 at 967.795 kb on - strand, within MMP_RS05085 at 967.853 kb on - strand, within MMP_RS05085 at 967.871 kb on - strand, within MMP_RS05085 at 967.892 kb on - strand, within MMP_RS05085 at 967.910 kb on - strand, within MMP_RS05085 at 967.911 kb on + strand, within MMP_RS05085 at 968.129 kb on - strand at 968.130 kb on + strand at 968.297 kb on + strand, within MMP_RS05090 at 968.482 kb on - strand, within MMP_RS05090 at 968.710 kb on - strand, within MMP_RS05090 at 968.901 kb on - strand, within MMP_RS05090 at 968.950 kb on - strand, within MMP_RS05090 at 968.951 kb on + strand, within MMP_RS05090 at 968.986 kb on + strand, within MMP_RS05090 at 969.055 kb on - strand, within MMP_RS05090 at 969.055 kb on - strand, within MMP_RS05090 at 969.056 kb on + strand, within MMP_RS05090 at 969.130 kb on - strand, within MMP_RS05090 at 969.215 kb on - strand, within MMP_RS05090 at 969.220 kb on + strand, within MMP_RS05090 at 969.247 kb on - strand, within MMP_RS05090 at 969.487 kb on - strand at 969.606 kb on - strand, within MMP_RS05095 at 969.607 kb on + strand, within MMP_RS05095 at 969.674 kb on + strand, within MMP_RS05095 at 969.674 kb on + strand, within MMP_RS05095 at 969.674 kb on + strand, within MMP_RS05095 at 969.694 kb on + strand, within MMP_RS05095 at 969.812 kb on + strand, within MMP_RS05095 at 970.159 kb on + strand at 970.171 kb on + strand at 970.182 kb on - strand at 970.212 kb on - strand at 970.212 kb on - strand at 970.212 kb on - strand at 970.213 kb on + strand at 970.213 kb on + strand at 970.216 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate deepwell autotrophic conditions remove 967,467 + MMP_RS05085 0.14 -3.5 967,483 + MMP_RS05085 0.16 -3.9 967,570 - MMP_RS05085 0.27 -4.8 967,732 + MMP_RS05085 0.49 -4.2 967,741 - MMP_RS05085 0.50 -2.8 967,742 + MMP_RS05085 0.50 -4.4 967,781 - MMP_RS05085 0.55 -2.8 967,795 - MMP_RS05085 0.57 -4.1 967,853 - MMP_RS05085 0.65 -4.2 967,871 - MMP_RS05085 0.67 -3.5 967,892 - MMP_RS05085 0.70 -4.0 967,910 - MMP_RS05085 0.72 -3.5 967,911 + MMP_RS05085 0.73 -4.8 968,129 - -3.5 968,130 + -5.0 968,297 + MMP_RS05090 0.12 -3.1 968,482 - MMP_RS05090 0.26 -3.3 968,710 - MMP_RS05090 0.42 -4.1 968,901 - MMP_RS05090 0.56 -5.3 968,950 - MMP_RS05090 0.60 -3.9 968,951 + MMP_RS05090 0.60 -4.7 968,986 + MMP_RS05090 0.62 -4.7 969,055 - MMP_RS05090 0.67 -4.1 969,055 - MMP_RS05090 0.67 -5.2 969,056 + MMP_RS05090 0.68 -3.8 969,130 - MMP_RS05090 0.73 -2.4 969,215 - MMP_RS05090 0.79 -4.8 969,220 + MMP_RS05090 0.79 -4.2 969,247 - MMP_RS05090 0.81 -4.3 969,487 - -3.6 969,606 - MMP_RS05095 0.18 -3.6 969,607 + MMP_RS05095 0.18 -3.1 969,674 + MMP_RS05095 0.31 -3.8 969,674 + MMP_RS05095 0.31 -3.1 969,674 + MMP_RS05095 0.31 -3.6 969,694 + MMP_RS05095 0.35 -3.9 969,812 + MMP_RS05095 0.58 -3.9 970,159 + -4.6 970,171 + -3.8 970,182 - -3.9 970,212 - -3.4 970,212 - -4.2 970,212 - -3.3 970,213 + -4.8 970,213 + -4.2 970,216 - -3.8
Or see this region's nucleotide sequence