Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_06559 and H281DRAFT_06560 are separated by 85 nucleotides H281DRAFT_06560 and H281DRAFT_06561 overlap by 10 nucleotides H281DRAFT_06561 and H281DRAFT_06562 are separated by 90 nucleotides H281DRAFT_06562 and H281DRAFT_06563 are separated by 236 nucleotides H281DRAFT_06563 and H281DRAFT_06564 are separated by 136 nucleotides
H281DRAFT_06559: H281DRAFT_06559 - hypothetical protein, at 1,586 to 1,891
_06559
H281DRAFT_06560: H281DRAFT_06560 - hypothetical protein, at 1,977 to 2,675
_06560
H281DRAFT_06561: H281DRAFT_06561 - Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain, at 2,666 to 3,037
_06561
H281DRAFT_06562: H281DRAFT_06562 - hypothetical protein, at 3,128 to 3,346
_06562
H281DRAFT_06563: H281DRAFT_06563 - hypothetical protein, at 3,583 to 3,846
_06563
H281DRAFT_06564: H281DRAFT_06564 - hypothetical protein, at 3,983 to 4,183
_06564
Position (kb)
2
3
4 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1.995 kb on - strand at 2.147 kb on - strand, within H281DRAFT_06560 at 2.315 kb on - strand, within H281DRAFT_06560 at 2.376 kb on + strand, within H281DRAFT_06560 at 2.613 kb on + strand at 2.613 kb on + strand at 2.613 kb on + strand at 2.614 kb on - strand at 2.642 kb on + strand at 2.674 kb on - strand at 2.708 kb on + strand, within H281DRAFT_06561 at 2.709 kb on - strand, within H281DRAFT_06561 at 2.787 kb on + strand, within H281DRAFT_06561 at 2.788 kb on - strand, within H281DRAFT_06561 at 2.788 kb on - strand, within H281DRAFT_06561 at 2.788 kb on - strand, within H281DRAFT_06561 at 2.788 kb on - strand, within H281DRAFT_06561 at 2.891 kb on + strand, within H281DRAFT_06561 at 2.891 kb on + strand, within H281DRAFT_06561 at 2.892 kb on - strand, within H281DRAFT_06561 at 2.917 kb on + strand, within H281DRAFT_06561 at 2.918 kb on - strand at 2.918 kb on - strand, within H281DRAFT_06561 at 2.918 kb on - strand, within H281DRAFT_06561 at 2.923 kb on + strand, within H281DRAFT_06561 at 2.924 kb on - strand, within H281DRAFT_06561 at 3.012 kb on + strand at 3.012 kb on + strand at 3.012 kb on + strand at 3.013 kb on - strand at 3.013 kb on - strand at 3.013 kb on - strand at 3.013 kb on - strand at 3.095 kb on + strand at 3.095 kb on + strand at 3.141 kb on + strand at 3.142 kb on - strand at 3.250 kb on + strand, within H281DRAFT_06562 at 3.334 kb on + strand at 3.346 kb on + strand at 3.346 kb on + strand at 3.346 kb on + strand at 3.346 kb on + strand at 3.346 kb on + strand at 3.346 kb on + strand at 3.346 kb on + strand at 3.346 kb on + strand at 3.347 kb on - strand at 3.347 kb on - strand at 3.347 kb on - strand at 3.347 kb on - strand at 3.347 kb on - strand at 3.348 kb on + strand at 3.349 kb on - strand at 3.349 kb on - strand at 3.356 kb on + strand at 3.357 kb on - strand at 3.357 kb on - strand at 3.357 kb on - strand at 3.375 kb on + strand at 3.375 kb on + strand at 3.376 kb on - strand at 3.376 kb on - strand at 3.376 kb on - strand at 3.430 kb on - strand at 3.435 kb on + strand at 3.436 kb on - strand at 3.558 kb on + strand at 3.559 kb on - strand at 3.563 kb on + strand at 3.563 kb on + strand at 3.568 kb on + strand at 3.568 kb on + strand at 3.682 kb on + strand, within H281DRAFT_06563 at 3.802 kb on + strand, within H281DRAFT_06563 at 3.803 kb on - strand, within H281DRAFT_06563 at 3.804 kb on + strand, within H281DRAFT_06563 at 3.805 kb on - strand, within H281DRAFT_06563 at 3.805 kb on - strand, within H281DRAFT_06563 at 3.873 kb on + strand at 3.873 kb on + strand at 3.874 kb on - strand at 3.940 kb on - strand at 3.971 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 1,995 - +0.8 2,147 - H281DRAFT_06560 0.24 -0.3 2,315 - H281DRAFT_06560 0.48 -1.3 2,376 + H281DRAFT_06560 0.57 -0.3 2,613 + -0.2 2,613 + -0.2 2,613 + +0.7 2,614 - +1.9 2,642 + +1.1 2,674 - -0.5 2,708 + H281DRAFT_06561 0.11 +0.5 2,709 - H281DRAFT_06561 0.12 +0.3 2,787 + H281DRAFT_06561 0.33 -0.1 2,788 - H281DRAFT_06561 0.33 +0.4 2,788 - H281DRAFT_06561 0.33 -0.9 2,788 - H281DRAFT_06561 0.33 +1.0 2,788 - H281DRAFT_06561 0.33 -0.1 2,891 + H281DRAFT_06561 0.60 +0.2 2,891 + H281DRAFT_06561 0.60 +1.0 2,892 - H281DRAFT_06561 0.61 +0.2 2,917 + H281DRAFT_06561 0.67 -0.1 2,918 - -0.5 2,918 - H281DRAFT_06561 0.68 +0.4 2,918 - H281DRAFT_06561 0.68 +1.1 2,923 + H281DRAFT_06561 0.69 +0.1 2,924 - H281DRAFT_06561 0.69 +0.6 3,012 + +0.3 3,012 + +0.1 3,012 + +0.5 3,013 - -0.1 3,013 - +0.0 3,013 - +0.9 3,013 - +2.7 3,095 + +1.3 3,095 + -0.1 3,141 + -0.4 3,142 - -0.2 3,250 + H281DRAFT_06562 0.56 -0.9 3,334 + +1.0 3,346 + +0.5 3,346 + -0.3 3,346 + +0.7 3,346 + +0.3 3,346 + -0.2 3,346 + -0.7 3,346 + +0.1 3,346 + +0.0 3,347 - -0.5 3,347 - +1.4 3,347 - -0.1 3,347 - -0.1 3,347 - +1.1 3,348 + +0.3 3,349 - -0.5 3,349 - +1.0 3,356 + +0.5 3,357 - -0.5 3,357 - -0.5 3,357 - +0.3 3,375 + +0.1 3,375 + -0.4 3,376 - -0.9 3,376 - -1.0 3,376 - -0.2 3,430 - -0.5 3,435 + -0.1 3,436 - -2.0 3,558 + +0.5 3,559 - -0.5 3,563 + -0.0 3,563 + -0.2 3,568 + -0.1 3,568 + +0.7 3,682 + H281DRAFT_06563 0.38 -0.2 3,802 + H281DRAFT_06563 0.83 +0.1 3,803 - H281DRAFT_06563 0.83 -0.5 3,804 + H281DRAFT_06563 0.84 -1.0 3,805 - H281DRAFT_06563 0.84 +0.9 3,805 - H281DRAFT_06563 0.84 -1.7 3,873 + +0.9 3,873 + -0.0 3,874 - -0.4 3,940 - +0.6 3,971 + +0.1
Or see this region's nucleotide sequence