Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05817 and H281DRAFT_05818 are separated by 32 nucleotides H281DRAFT_05818 and H281DRAFT_05819 overlap by 4 nucleotides
H281DRAFT_05817: H281DRAFT_05817 - NADH dehydrogenase, at 94,285 to 95,238
_05817
H281DRAFT_05818: H281DRAFT_05818 - glutathione S-transferase, at 95,271 to 95,924
_05818
H281DRAFT_05819: H281DRAFT_05819 - tRNA nucleotidyltransferase (CCA-adding enzyme), at 95,921 to 97,153
_05819
Position (kb) 95
96 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 94.396 kb on + strand, within H281DRAFT_05817 at 94.396 kb on + strand, within H281DRAFT_05817 at 94.397 kb on - strand at 94.397 kb on - strand, within H281DRAFT_05817 at 94.398 kb on + strand, within H281DRAFT_05817 at 94.398 kb on + strand, within H281DRAFT_05817 at 94.425 kb on + strand, within H281DRAFT_05817 at 94.500 kb on + strand, within H281DRAFT_05817 at 94.849 kb on + strand, within H281DRAFT_05817 at 94.850 kb on - strand, within H281DRAFT_05817 at 94.940 kb on - strand, within H281DRAFT_05817 at 94.964 kb on - strand, within H281DRAFT_05817 at 95.260 kb on + strand at 95.260 kb on + strand at 95.260 kb on + strand at 95.261 kb on - strand at 95.261 kb on - strand at 95.282 kb on + strand at 95.282 kb on + strand at 95.282 kb on + strand at 95.282 kb on + strand at 95.282 kb on + strand at 95.298 kb on + strand at 95.609 kb on + strand, within H281DRAFT_05818 at 95.715 kb on + strand, within H281DRAFT_05818 at 95.715 kb on + strand, within H281DRAFT_05818 at 95.715 kb on + strand, within H281DRAFT_05818 at 95.715 kb on + strand, within H281DRAFT_05818 at 95.715 kb on + strand, within H281DRAFT_05818 at 95.715 kb on + strand, within H281DRAFT_05818 at 95.716 kb on - strand, within H281DRAFT_05818 at 95.716 kb on - strand, within H281DRAFT_05818 at 95.763 kb on + strand, within H281DRAFT_05818 at 95.763 kb on + strand, within H281DRAFT_05818 at 95.764 kb on - strand, within H281DRAFT_05818 at 95.764 kb on - strand, within H281DRAFT_05818 at 95.826 kb on + strand, within H281DRAFT_05818 at 95.827 kb on - strand, within H281DRAFT_05818 at 95.828 kb on + strand, within H281DRAFT_05818 at 95.828 kb on + strand, within H281DRAFT_05818 at 95.904 kb on + strand at 95.905 kb on - strand at 95.908 kb on + strand at 95.908 kb on + strand at 95.926 kb on + strand at 95.926 kb on + strand at 95.930 kb on + strand at 95.930 kb on + strand at 96.727 kb on + strand, within H281DRAFT_05819 at 96.742 kb on + strand, within H281DRAFT_05819
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 94,396 + H281DRAFT_05817 0.12 +0.3 94,396 + H281DRAFT_05817 0.12 +2.4 94,397 - -1.0 94,397 - H281DRAFT_05817 0.12 -2.9 94,398 + H281DRAFT_05817 0.12 +0.5 94,398 + H281DRAFT_05817 0.12 +0.6 94,425 + H281DRAFT_05817 0.15 -0.6 94,500 + H281DRAFT_05817 0.23 +0.3 94,849 + H281DRAFT_05817 0.59 -0.1 94,850 - H281DRAFT_05817 0.59 -0.7 94,940 - H281DRAFT_05817 0.69 -0.8 94,964 - H281DRAFT_05817 0.71 -0.7 95,260 + +0.2 95,260 + +0.5 95,260 + -0.4 95,261 - -0.1 95,261 - -0.9 95,282 + -0.5 95,282 + -0.6 95,282 + +0.7 95,282 + -0.1 95,282 + -0.2 95,298 + -1.1 95,609 + H281DRAFT_05818 0.52 -0.5 95,715 + H281DRAFT_05818 0.68 +0.1 95,715 + H281DRAFT_05818 0.68 -0.1 95,715 + H281DRAFT_05818 0.68 +0.3 95,715 + H281DRAFT_05818 0.68 +0.5 95,715 + H281DRAFT_05818 0.68 -0.1 95,715 + H281DRAFT_05818 0.68 -0.3 95,716 - H281DRAFT_05818 0.68 -1.0 95,716 - H281DRAFT_05818 0.68 -2.8 95,763 + H281DRAFT_05818 0.75 -0.7 95,763 + H281DRAFT_05818 0.75 +0.1 95,764 - H281DRAFT_05818 0.75 -0.6 95,764 - H281DRAFT_05818 0.75 -0.0 95,826 + H281DRAFT_05818 0.85 -0.7 95,827 - H281DRAFT_05818 0.85 -2.0 95,828 + H281DRAFT_05818 0.85 -0.8 95,828 + H281DRAFT_05818 0.85 +0.0 95,904 + -0.5 95,905 - -0.2 95,908 + -1.0 95,908 + -1.5 95,926 + -0.5 95,926 + -2.2 95,930 + -0.4 95,930 + +0.2 96,727 + H281DRAFT_05819 0.65 -0.2 96,742 + H281DRAFT_05819 0.67 -1.5
Or see this region's nucleotide sequence