Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05723 and H281DRAFT_05724 are separated by 121 nucleotides H281DRAFT_05724 and H281DRAFT_05725 are separated by 131 nucleotides
H281DRAFT_05723: H281DRAFT_05723 - bifunctional thiolase PaaJ: 3-oxo-5,6-didehydrosuberyl-CoA thiolase (EC 2.3.1.223); 3-oxoadipyl-CoA thiolase (EC 2.3.1.174) (from data) , at 141,891 to 143,093
_05723
H281DRAFT_05724: H281DRAFT_05724 - 3-oxo-5,6-didehydrosuberyl-CoA semialdehyde dehydrogenase PaaZ (EC:1.2.1.91) (from data) , at 143,215 to 144,915
_05724
H281DRAFT_05725: H281DRAFT_05725 - 2,3-dehydroadipyl-CoA hydratase / enoyl-CoA hydratase (EC 4.2.1.17) (from data) , at 145,047 to 145,823
_05725
Position (kb)
143
144
145 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 142.276 kb on + strand, within H281DRAFT_05723 at 142.276 kb on + strand, within H281DRAFT_05723 at 142.353 kb on + strand, within H281DRAFT_05723 at 142.354 kb on - strand, within H281DRAFT_05723 at 142.374 kb on + strand, within H281DRAFT_05723 at 142.374 kb on + strand, within H281DRAFT_05723 at 142.374 kb on + strand, within H281DRAFT_05723 at 142.375 kb on - strand, within H281DRAFT_05723 at 142.375 kb on - strand, within H281DRAFT_05723 at 142.375 kb on - strand, within H281DRAFT_05723 at 142.375 kb on - strand, within H281DRAFT_05723 at 142.375 kb on - strand, within H281DRAFT_05723 at 142.837 kb on - strand, within H281DRAFT_05723 at 142.933 kb on + strand, within H281DRAFT_05723 at 142.933 kb on + strand, within H281DRAFT_05723 at 142.934 kb on - strand, within H281DRAFT_05723 at 142.934 kb on - strand, within H281DRAFT_05723 at 142.934 kb on - strand, within H281DRAFT_05723 at 142.974 kb on - strand at 143.078 kb on + strand at 143.078 kb on + strand at 143.080 kb on + strand at 143.081 kb on - strand at 143.094 kb on - strand at 143.094 kb on - strand at 143.105 kb on + strand at 143.144 kb on + strand at 143.144 kb on + strand at 143.145 kb on - strand at 143.145 kb on - strand at 143.145 kb on - strand at 143.145 kb on - strand at 143.145 kb on - strand at 143.145 kb on - strand at 143.145 kb on - strand at 143.145 kb on - strand at 143.145 kb on - strand at 143.184 kb on + strand at 143.185 kb on - strand at 143.185 kb on - strand at 143.207 kb on + strand at 143.207 kb on + strand at 143.225 kb on - strand at 143.401 kb on + strand, within H281DRAFT_05724 at 143.867 kb on + strand, within H281DRAFT_05724 at 143.867 kb on + strand, within H281DRAFT_05724 at 143.867 kb on + strand, within H281DRAFT_05724 at 143.867 kb on + strand, within H281DRAFT_05724 at 143.867 kb on + strand, within H281DRAFT_05724 at 143.996 kb on + strand, within H281DRAFT_05724 at 143.996 kb on + strand, within H281DRAFT_05724 at 144.127 kb on + strand, within H281DRAFT_05724 at 144.166 kb on + strand, within H281DRAFT_05724 at 144.166 kb on + strand, within H281DRAFT_05724 at 144.431 kb on + strand, within H281DRAFT_05724 at 144.431 kb on + strand, within H281DRAFT_05724 at 144.522 kb on + strand, within H281DRAFT_05724 at 144.522 kb on + strand, within H281DRAFT_05724 at 144.522 kb on + strand, within H281DRAFT_05724 at 144.660 kb on + strand, within H281DRAFT_05724 at 144.697 kb on + strand, within H281DRAFT_05724 at 144.698 kb on - strand, within H281DRAFT_05724 at 144.933 kb on - strand at 144.954 kb on - strand at 144.954 kb on - strand at 144.957 kb on - strand at 144.957 kb on - strand at 144.993 kb on - strand at 144.996 kb on - strand at 144.996 kb on - strand at 144.996 kb on - strand at 144.996 kb on - strand at 144.996 kb on - strand at 144.996 kb on - strand at 145.036 kb on + strand at 145.036 kb on + strand at 145.037 kb on - strand at 145.053 kb on + strand at 145.264 kb on - strand, within H281DRAFT_05725 at 145.264 kb on - strand, within H281DRAFT_05725 at 145.269 kb on + strand, within H281DRAFT_05725 at 145.269 kb on + strand, within H281DRAFT_05725 at 145.269 kb on + strand, within H281DRAFT_05725 at 145.269 kb on + strand, within H281DRAFT_05725 at 145.269 kb on + strand, within H281DRAFT_05725 at 145.269 kb on + strand, within H281DRAFT_05725 at 145.269 kb on + strand at 145.270 kb on - strand, within H281DRAFT_05725 at 145.270 kb on - strand, within H281DRAFT_05725 at 145.271 kb on + strand, within H281DRAFT_05725 at 145.272 kb on - strand, within H281DRAFT_05725 at 145.272 kb on - strand, within H281DRAFT_05725 at 145.281 kb on + strand, within H281DRAFT_05725 at 145.282 kb on - strand, within H281DRAFT_05725 at 145.282 kb on - strand, within H281DRAFT_05725 at 145.282 kb on - strand, within H281DRAFT_05725 at 145.282 kb on - strand, within H281DRAFT_05725 at 145.293 kb on + strand at 145.293 kb on + strand, within H281DRAFT_05725 at 145.294 kb on - strand, within H281DRAFT_05725 at 145.294 kb on - strand, within H281DRAFT_05725 at 145.294 kb on - strand, within H281DRAFT_05725 at 145.304 kb on + strand, within H281DRAFT_05725 at 145.304 kb on + strand, within H281DRAFT_05725 at 145.305 kb on - strand, within H281DRAFT_05725 at 145.331 kb on + strand, within H281DRAFT_05725 at 145.331 kb on + strand, within H281DRAFT_05725 at 145.332 kb on - strand, within H281DRAFT_05725 at 145.509 kb on - strand, within H281DRAFT_05725 at 145.542 kb on - strand, within H281DRAFT_05725 at 145.542 kb on - strand, within H281DRAFT_05725 at 145.635 kb on - strand, within H281DRAFT_05725 at 145.824 kb on + strand at 145.824 kb on + strand at 145.824 kb on + strand at 145.824 kb on + strand at 145.839 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 142,276 + H281DRAFT_05723 0.32 -0.6 142,276 + H281DRAFT_05723 0.32 +2.4 142,353 + H281DRAFT_05723 0.38 +0.2 142,354 - H281DRAFT_05723 0.38 +0.2 142,374 + H281DRAFT_05723 0.40 -0.2 142,374 + H281DRAFT_05723 0.40 +1.5 142,374 + H281DRAFT_05723 0.40 +0.2 142,375 - H281DRAFT_05723 0.40 +0.5 142,375 - H281DRAFT_05723 0.40 +0.0 142,375 - H281DRAFT_05723 0.40 -0.3 142,375 - H281DRAFT_05723 0.40 +0.3 142,375 - H281DRAFT_05723 0.40 -0.5 142,837 - H281DRAFT_05723 0.79 -0.0 142,933 + H281DRAFT_05723 0.87 +0.4 142,933 + H281DRAFT_05723 0.87 +0.2 142,934 - H281DRAFT_05723 0.87 +0.5 142,934 - H281DRAFT_05723 0.87 +0.1 142,934 - H281DRAFT_05723 0.87 +1.2 142,974 - -1.3 143,078 + +0.1 143,078 + +1.1 143,080 + -1.9 143,081 - +0.2 143,094 - -0.1 143,094 - +2.6 143,105 + +0.3 143,144 + +1.1 143,144 + +0.8 143,145 - -0.0 143,145 - +0.2 143,145 - +0.4 143,145 - -1.2 143,145 - +0.9 143,145 - +0.2 143,145 - +0.1 143,145 - -0.6 143,145 - -0.6 143,184 + +0.6 143,185 - +0.1 143,185 - +0.4 143,207 + +0.7 143,207 + +0.7 143,225 - +0.6 143,401 + H281DRAFT_05724 0.11 +0.3 143,867 + H281DRAFT_05724 0.38 +1.0 143,867 + H281DRAFT_05724 0.38 +0.2 143,867 + H281DRAFT_05724 0.38 +2.4 143,867 + H281DRAFT_05724 0.38 +1.0 143,867 + H281DRAFT_05724 0.38 +0.7 143,996 + H281DRAFT_05724 0.46 +0.3 143,996 + H281DRAFT_05724 0.46 -0.9 144,127 + H281DRAFT_05724 0.54 +0.6 144,166 + H281DRAFT_05724 0.56 +0.6 144,166 + H281DRAFT_05724 0.56 +0.7 144,431 + H281DRAFT_05724 0.71 -0.0 144,431 + H281DRAFT_05724 0.71 +2.1 144,522 + H281DRAFT_05724 0.77 +1.7 144,522 + H281DRAFT_05724 0.77 +0.8 144,522 + H281DRAFT_05724 0.77 +1.1 144,660 + H281DRAFT_05724 0.85 +1.6 144,697 + H281DRAFT_05724 0.87 +0.4 144,698 - H281DRAFT_05724 0.87 +0.9 144,933 - +0.4 144,954 - +0.5 144,954 - +0.8 144,957 - -0.3 144,957 - +0.0 144,993 - -1.4 144,996 - -0.3 144,996 - -1.4 144,996 - -1.4 144,996 - +0.2 144,996 - +0.1 144,996 - -0.3 145,036 + +0.3 145,036 + +0.3 145,037 - +0.2 145,053 + +0.1 145,264 - H281DRAFT_05725 0.28 -0.2 145,264 - H281DRAFT_05725 0.28 +0.1 145,269 + H281DRAFT_05725 0.29 +0.2 145,269 + H281DRAFT_05725 0.29 -0.8 145,269 + H281DRAFT_05725 0.29 +2.1 145,269 + H281DRAFT_05725 0.29 +0.4 145,269 + H281DRAFT_05725 0.29 +0.0 145,269 + H281DRAFT_05725 0.29 +1.5 145,269 + +1.1 145,270 - H281DRAFT_05725 0.29 -0.3 145,270 - H281DRAFT_05725 0.29 +0.6 145,271 + H281DRAFT_05725 0.29 -0.4 145,272 - H281DRAFT_05725 0.29 +0.7 145,272 - H281DRAFT_05725 0.29 -0.4 145,281 + H281DRAFT_05725 0.30 +0.4 145,282 - H281DRAFT_05725 0.30 -0.2 145,282 - H281DRAFT_05725 0.30 -0.1 145,282 - H281DRAFT_05725 0.30 +0.7 145,282 - H281DRAFT_05725 0.30 +0.1 145,293 + +1.1 145,293 + H281DRAFT_05725 0.32 +0.0 145,294 - H281DRAFT_05725 0.32 +0.3 145,294 - H281DRAFT_05725 0.32 +0.8 145,294 - H281DRAFT_05725 0.32 +0.9 145,304 + H281DRAFT_05725 0.33 +0.6 145,304 + H281DRAFT_05725 0.33 +1.2 145,305 - H281DRAFT_05725 0.33 +0.2 145,331 + H281DRAFT_05725 0.37 -0.7 145,331 + H281DRAFT_05725 0.37 +0.1 145,332 - H281DRAFT_05725 0.37 -0.1 145,509 - H281DRAFT_05725 0.59 -0.7 145,542 - H281DRAFT_05725 0.64 +0.2 145,542 - H281DRAFT_05725 0.64 +0.6 145,635 - H281DRAFT_05725 0.76 -0.6 145,824 + -0.2 145,824 + -0.2 145,824 + +0.5 145,824 + +0.6 145,839 - +0.0
Or see this region's nucleotide sequence